Disease | Research Area | St John's Laboratory

Western blot analysis of 3T3 cells using Phospho-Met (Y1356) Polyclonal Antibody

Anti-Phospho-MET-Tyr1356 antibody (1331-1380 aa) (STJ90469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of 1 HeLa cell, HepG2 cell, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MET-Tyr1365 antibody (STJA0010033)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HeLa cells, using Phospho-MET-Y1234/1235 Rabbit pAb (STJ11100968) at 1:1000 dilution. HeLa cells were treated by HGF (40 ng/ml) at 37 °C for 10 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-MET-Y1234/1235 antibody (STJ11100968)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of various lysates, using Phospho-Met-Y1234/Y1235 antibody (STJ11105828) at1:2000 dilution. C2C12 and C6 cells were treated by HGF (40 ng/ml) at 37 °C for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-MET-Y1234/Y1235 antibody [S5828RM] (STJ11105828)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from HeLa cells, using Phospho-MET-Y1349 Rabbit pAb (STJ22291) at 1:1000 dilution. HeLa cells were treated by HGF (40ng/mL) for 10 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-MET-Y1349 antibody (STJ22291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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Anti-Phospho-MLKL antibody (350-400) (STJ506097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-Phospho-MLKL antibody (350-400) {Biotin} (STJ506098)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£551.50

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Anti-Phospho-MLKL antibody (350-400) {FITC} (STJ506099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£551.50

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Anti-Phospho-MLKL-S358 antibody (STJ11108414)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£0.00

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-Phospho-MLKL-S358 antibody (STJ193224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, phospho-MLKL (S358) Mouse monoclonal antibody (6F8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Phospho-MLKL-S358 antibody [6F8] (STJ97776)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC

Reactivity :

Human

£49.00 - £261.00

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Anti-Phospho-MLKL-Ser345 antibody [PT0987R] (STJA0039926)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Western blot analysis of 3T3 cells using Phospho-MRE11 (S264) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-MRE11-Ser264 antibody (230-279 aa) (STJ90554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HeLa cells using Phospho-MRE11 (Ser676) Rabbit polyclonal antibody (STJ11105909) at 1:800 dilution. HeLa cells were treated by Calyculin A (200 nM) at 37 °C for 18 hours after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-MRE11-Ser676 antibody (STJ11105909)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£126.50 - £618.50

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-MRE11-Ser676 antibody (STJ196358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from C6 cells, using Phospho-mTOR-S2448 Rabbit pAb (STJ27512) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-mTOR-S2448 antibody (STJ27512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from 293T cells, using Phospho-mTOR-S2448 Rabbit mAb (STJ11102554) at 1:1000 dilution. 293T cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight or treated by IGF-1 (50 ng/mL) at 37 °C for 5 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-mTOR-S2448 antibody [S4MR] (STJ11102554)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of lysates from C6 cells using Phospho-mTOR-S2481 Rabbit pAb (STJ11102557) at 1:2000 dilution. C6 cells were treated by IGF-1 (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-mTOR-S2481 antibody (STJ11102557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-mTOR-S2481 (STJ11102570) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by IGF-1 (50 ng/mL) at 37 °C for 5 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-MTOR-S2481 antibody [S0MR] (STJ11102570)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunofluorescence analysis of Hela cell. 1, mTOR (phospho Ser2448) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MTOR-Ser2448 antibody (2415-2464 aa) (STJ90336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Cow/Pig

£49.00 - £261.00

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Anti-Phospho-mTOR-Ser2448 antibody [PT0498R] (STJA0039463)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of hela-UV using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-MTOR-Ser2481 antibody (2447-2496 aa) (STJ91294)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Cow

£49.00 - £261.00

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Anti-Phospho-mTOR-Ser2481 antibody [PT0898R] (STJA0039791)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of various cells using Phospho-mTOR (T2446) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MTOR-Thr2446 antibody (2412-2461 aa) (STJ90544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-MUC1-Ser1227 antibody (1196-1245 aa) (STJ91261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of HepG2 cells, using CD227/MUC1 (Phospho-Tyr1229) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MUC1-Tyr1229 antibody (1201-1250 aa) (STJ91106)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CD227/Mucin 1 (Phospho-Tyr1243) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MUC1-Tyr1243 antibody (1204-1253 aa) (STJ91141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from A431 cells using Phospho-c-Myc-S373 Rabbit pAb (STJ22301). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MYC-S373 antibody (STJ22301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-c-Myc-S62 Rabbit pAb (STJ22299) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-MYC-S62 antibody (STJ22299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-c-Myc-S62 Rabbit mAb (STJ11102580) at 1:1000 dilution. 293T cells were treated by Forskolin (10 uM) at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-MYC-S62 antibody [S0MR] (STJ11102580)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myc (Phospho-Ser373) Antibody.

Anti-Phospho-MYC-Ser373 antibody (340-389 aa) (STJ90227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-c-Myc (S62) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-MYC-Ser62 antibody (31-80 aa) (STJ91021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HT-29 cells, using Phospho-c-Myc-T358 Rabbit pAb (STJ22303). HT-29 cells treated by UV. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MYC-T358 antibody (STJ22303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from 293T cells, using Phospho-c-Myc-T58 Rabbit pAb (STJ22298) at 1:500 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-MYC-T58 antibody (STJ22298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-c-Myc-T58 Rabbit mAb (STJ11102581) at 1:1000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-MYC-T58 antibody [S1MR] (STJ11102581)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£143.50 - £618.50

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Western blot analysis of lysates from C6 cells, using Phospho-c-Myc-T58/S62 Rabbit mAb (STJ11102579) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-MYC-T58/S62 antibody [S9MR] (STJ11102579)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myc (Phospho-Thr358) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MYC-Thr358 antibody (325-374 aa) (STJ90228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-c-Myc (T58) Polyclonal Antibody diluted at 1：500

Anti-Phospho-MYC-Thr58 antibody (25-74 aa) (STJ90229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of 1 A431 cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-MYD88-Tyr257 antibody (STJA0009895)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from 293T cells, using Phospho-MYH9-S1943 Rabbit pAb (STJ117900) at 1:2000 dilution. 293T cell lysates were treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-MYH9-S1943 antibody (STJ117900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-MYH9-S1943 antibody (STJA0005473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£367.50

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Anti-Phospho-MYH9-Ser-1803 antibody (STJA0005471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat

£367.50

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Anti-Phospho-MYH9-Ser-1916 antibody (STJA0005472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat

£367.50

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Anti-Phospho-MYH9-Ser1943 antibody (STJ196359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-MYH9-Ser1943 antibody (STJ196360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-NACA-Ser43 antibody (9-58 aa) (STJ91263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-NACA-Thr159 antibody (STJA0010000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HUVEC cells treated with Forskolin 40nM 30', using Nibrin (Phospho-Ser278) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-NBN-Ser278 antibody (251-300 aa) (STJ90727)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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