• Chromatin immunoprecipitation analysis of extracts of K562 cells, using c-Myc antibody (STJ11101570) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Western blot analysis of lysates from wild type (WT) and c-Myc knockout (KO) HCT 116 cells, using [KO Validated] c-Myc Rabbit monoclonal antibody (STJ11101570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from Mouse ovary, using [KO Validated] c-Myc Rabbit monoclonal antibody (STJ11101570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of various lysates using [KO Validated] c-Myc Rabbit monoclonal antibody (STJ11101570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • UT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g c-Myc Rabbit monoclonal antibody (STJ11101570) , along with a Goat Anti-Rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of ESE1 in representative gene loci (NPM1) , as shown in figure.

Anti-MYC antibody (1-100) [S0MR] (STJ11101570)

SKU:
STJ11101570

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-c-Myc (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
ChIP 1:50-1:200
CUT&Tag 10⁵ cells/1 Mu g
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYC
Gene ID: 4609
Uniprot ID: MYC_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human c-Myc (P01106).
Immunogen Sequence: MPLNVSFTNRNYDLDYDSVQ PYFYCDEEENFYQQQQQSEL QPPAPSEDIWKKFELLPTPP LSPSRRSGLCSPSYVAVTPF SLRGDNDGGGGSFSTADQLE
Post Translational Modifications Phosphorylated by PRKDC. Phosphorylation at Ser-344 by PIM2 leads to the stabilization of MYC. Phosphorylation at Ser-77 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-77 by DYRK2.this primes the protein for subsequent phosphorylation by GSK3B at Thr-73. Phosphorylation at Thr-73 and Ser-77 by GSK3 is required for ubiquitination and degradation by the proteasome. Dephosphorylation at Ser-77 by protein phosphatase 2A (PPP2CA) promotes its degradation.interaction with PPP2CA is enhanced by AMBRA1. Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-73 and Ser-77, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28 but by USP36, due to the lack of interaction between isoform 3 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRPC4AP) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner.
Function Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CACGATG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis. Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells. Functions with TAF6L to activate target gene expression through RNA polymerase II pause release. Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform.
Protein Name Myc Proto-Oncogene Protein
Class E Basic Helix-Loop-Helix Protein 39
Bhlhe39
Proto-Oncogene C-Myc
Transcription Factor P64
Database Links Reactome: R-HSA-1362277
Reactome: R-HSA-201556
Reactome: R-HSA-2122947
Reactome: R-HSA-2173796
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-4411364
Reactome: R-HSA-5687128
Reactome: R-HSA-5689880
Reactome: R-HSA-6785807
Reactome: R-HSA-69202
Reactome: R-HSA-69656
Reactome: R-HSA-8866911
Reactome: R-HSA-8951430
Reactome: R-HSA-9018519
Reactome: R-HSA-9616222
Reactome: R-HSA-9818749
Cellular Localisation Nucleus
Nucleoplasm
Nucleolus
Alternative Antibody Names Anti-Myc Proto-Oncogene Protein antibody
Anti-Class E Basic Helix-Loop-Helix Protein 39 antibody
Anti-Bhlhe39 antibody
Anti-Proto-Oncogene C-Myc antibody
Anti-Transcription Factor P64 antibody
Anti-MYC antibody
Anti-BHLHE39 antibody

Information sourced from Uniprot.org

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