Anti-Myc proto-oncogene protein antibody (EQKLISEEDL) [Myc.A7] (STJA0040740)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenA synthetic peptide (EQKLISEEDL) coupled to KLH.
General Information
| Short Description | Mouse monoclonal anti-Myc proto-oncogene protein (EQKLISEEDL) for use in ICC and WB in Species Independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/WB |
| Host | Mouse |
| Reactivity | Species Independent |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | Myc.A7 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Affinity-purified |
| Dilution Range | WB 0.73611111111111116IHC 0.18055555555555555ICC 0.18055555555555555IP 0.18055555555555555 |
| Formulation | PBS, pH 7.4 with 0.05% sodium azide |
| Storage Instruction | Stable for 1 year at-20°C from the date of receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. |
Target Information
| Gene Symbol | MYC |
| Gene ID | 4609 |
| Uniprot ID | MYC_HUMAN |
| Immunogen | A synthetic peptide (EQKLISEEDL) coupled to KLH. |
| Immunogen Region | EQKLISEEDL |
| Immunogen Sequence | Human |
| Specificity | Antibody detects over-expressed proteins containing Myc epitope tag fused to either amino-or carboxy-termini of targeted proteins in transfected mammalian cells. |
Additional Info
| Post Translational Modifications | Phosphorylated by PRKDC. Phosphorylation at Ser-344 by PIM2 leads to the stabilization of MYC. Phosphorylation at Ser-77 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-77 by DYRK2.this primes the protein for subsequent phosphorylation by GSK3B at Thr-73. Phosphorylation at Thr-73 and Ser-77 by GSK3 is required for ubiquitination and degradation by the proteasome. Dephosphorylation at multiple sites by the PNUTS-PP1 complex promotes MYC stability by preventing ubiquitination by the SCF(FBXW7) complex. Dephosphorylation at Ser-77 by protein phosphatase 2A (PPP2CA) promotes its degradation.interaction with PPP2CA is enhanced by AMBRA1. Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-73 and Ser-77, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28 but by USP36, due to the lack of interaction between isoform 3 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRPC4AP) complex. Ubiquitinated by UBR5 when not forming a heterodimer with another bHLH protein, leading to its degradation: UBR5 recognizes and binds a degron that is only available upon heterodimer dissociation. Ubiquitinated by TRIM6 in a phosphorylation-independent manner. |
| Function | Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CACGATG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis. Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells. Functions with TAF6L to activate target gene expression through RNA polymerase II pause release. Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform. |
| Protein Name | Myc Proto-Oncogene ProteinClass E Basic Helix-Loop-Helix Protein 39Bhlhe39Proto-Oncogene C-MycTranscription Factor P64 |
| Database Links | Reactome: R-HSA-1362277Reactome: R-HSA-201556Reactome: R-HSA-2122947Reactome: R-HSA-2173796Reactome: R-HSA-2644606Reactome: R-HSA-2894862Reactome: R-HSA-4411364Reactome: R-HSA-5687128Reactome: R-HSA-5689880Reactome: R-HSA-6785807Reactome: R-HSA-69202Reactome: R-HSA-69656Reactome: R-HSA-8866911Reactome: R-HSA-8951430Reactome: R-HSA-9018519Reactome: R-HSA-9616222Reactome: R-HSA-9818749 |
| Cellular Localisation | NucleusNucleoplasmNucleolusCytoplasmChromosomeAssociation With Chromatin Is Reduced By HyperphosphorylationLocalization To The Nucleolus Is Dependent On Heatr1 |
| Alternative Antibody Names | Anti-Myc Proto-Oncogene Protein antibodyAnti-Class E Basic Helix-Loop-Helix Protein 39 antibodyAnti-Bhlhe39 antibodyAnti-Proto-Oncogene C-Myc antibodyAnti-Transcription Factor P64 antibodyAnti-MYC antibodyAnti-BHLHE39 antibody |
Information sourced from Uniprot.org