Developmental Biology | Research Area | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1, FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Phospho-FOXO3-Ser253 antibody (220-269 aa) (STJ90275)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Zebrafish

£48.00 - £252.00

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Western blot analysis of Phospho-FOXO3A (Ser253) in 3T3 lysates using Phospho-FOXO3A (Ser253) antibody.

Anti-Phospho-FOXO3-Ser253 antibody (248-260 aa) [R06-2G6] (STJA0011330)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£246.50

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Anti-Phospho-FOXO3-Ser294 antibody (STJ196308)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-FOXO3-Ser315 antibody (STJA0009840)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of 1 HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-FOXO3-Ser318 antibody (STJA0010058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Phospho-FOXO3-Ser318/321 antibody (STJ196309)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of 1 A549 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-FOXO3-Ser321 antibody (STJA0010059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Phospho-FOXO3-Ser413 antibody (STJ196310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-FOXO3-Ser425 antibody (STJ196311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-FOXO3-Ser644 antibody (STJA0010017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Phospho-FOXO3-Ser7 antibody (STJ196312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from K-562 cells, using Phospho-FOXO3A-S425 Rabbit pAb (STJ11103738) at 1:1000 dilution. K-562 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-FOXO3A-S425 antibody (STJ11103738)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-FOXO4-S193 antibody (STJ11107664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of various lysates, using Phospho-FOXO4-S197 Rabbit pAb (STJ22147) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-FOXO4-S197 antibody (STJ22147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-FOXO4-Ser193 antibody (STJ196313)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-FoxO4 (S197) Polyclonal Antibody

Anti-Phospho-FOXO4-Ser197 antibody (164-213 aa) (STJ90276)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Jurkat cells treated with starved 24h, using FOXO4 (Phospho-Ser262) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-FOXO4-Ser262 antibody (228-277 aa) (STJ90930)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 5', using FOXO4 (Phospho-Thr451) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-FOXO4-Thr451 antibody (417-466 aa) (STJ90542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-FRS2-Tyr196 antibody (STJ196314)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-FRS2 (Y436) Polyclonal Antibody

Anti-Phospho-FRS2-Tyr436 antibody (402-451 aa) (STJ90965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from A-431 cells, using Phospho-Fyn-S21 Rabbit pAb (STJ110850) at 1:1000 dilution. A-431 cells were treated by 10% FBS after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-FYN-S21 antibody (STJ110850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of LOVO+UV cells using Phospho-Fyn (Y530) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-FYN-Tyr530 antibody (488-537 aa) (STJ90792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-GAB1-Tyr307 antibody (STJ91230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Gab 1 (Y627) Polyclonal Antibody

Anti-Phospho-GAB1-Tyr627 antibody (611-643 aa) (STJ90278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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Western blot analysis of 3T3 cells using Phospho-Gab 1 (Y659) Polyclonal Antibody

Anti-Phospho-GAB1-Tyr659 antibody (644-674 aa) (STJ91067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-GAB1-Y307 Rabbit polyclonal antibody (STJ11105790) at 1:1000 dilution. NIH/3T3 cells were treated by IGF-1 (50 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-GAB1-Y307 antibody (STJ11105790)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£129.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-GAB1-Y659 Rabbit pAb (STJ116394) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-GAB1-Y659 antibody (STJ116394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of lysates from HT29 cells treated with serum 20% 15', using GRB2 (Phospho-Ser159) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-GAB2-Ser159 antibody (125-174 aa) (STJ90967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Gab2 (Phospho-Ser623) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-GAB2-Ser623 antibody (589-638 aa) (STJ90978)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-GAB2-Tyr452 antibody (STJ90630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of 1 HeLa cell, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-GAB2-Tyr614 antibody (STJA0010078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using Gab2 (Phospho-Tyr643) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-GAB2-Tyr643 antibody (609-658 aa) (STJ90977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-Gamma-H2AX-S139 antibody [9F3] (STJA0019679)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-Gamma-H2AX-S139 antibody [A4] (STJA0019153)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-Gamma-H2AX-S139 antibody [A9] (STJA0019152)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-Gamma-H2AX-S139 antibody [C6] (STJA0019151)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-Gamma-H2AX-S139 antibody [G2] (STJA0019154)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-GAP43-S41 antibody (STJ13101125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-Phospho-GAP43-S41 antibody (STJ13101126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-Phospho-GAP43-S41 antibody (STJ13101133)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-Phospho-GAP43-S41 antibody (STJ13101134)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC

Reactivity :

Human/Rat/Mouse

£331.50

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Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-GAP43-S41 antibody (STJ22150).

Anti-Phospho-GAP43-S41 antibody (STJ22150)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of MCF-7 cells, using GAP43 (Phospho-Ser41) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-GAP43-Ser41 antibody (8-57 aa) (STJ90279)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from COS7 cells treated with TNF 20ng/ml 5', using GATA2 (Phospho-Ser401) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-GATA2-Ser401 antibody (367-416 aa) (STJ90518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Monkey

£48.00 - £252.00

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Immunofluorescence analysis of HUVEC cells, using GATA3 (Phospho-Ser308) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-GATA3-Ser308 antibody (274-323 aa) (STJ91339)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of HepG2 cells, using GATA4 (Phospho-Ser105) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-GATA4-Ser105 antibody (71-120 aa) (STJ91340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from 293 cells, using GATA4 (Phospho-Ser262) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-GATA4-Ser262 antibody (228-277 aa) (STJ90714)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-GRB10-Ser476 antibody (STJ196318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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