• Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g hnRNP U antibody (STJ24066). Western blot was performed from the immunoprecipitate using hnRNP U antibody (STJ24066) at a dilution of 1:1000.
  • Immunofluorescence analysis of C6 cells using hnRNP U Rabbit polyclonal antibody (STJ24066) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of hnRNP U in paraffin-embedded Human esophageal cancer using hnRNP U Rabbit polyclonal antibody (STJ24066) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of hnRNP U in paraffin-embedded Rat testis using hnRNP U Rabbit polyclonal antibody (STJ24066) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of hnRNP U in paraffin-embedded Mouse brain using hnRNP U Rabbit polyclonal antibody (STJ24066) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using hnRNP U Rabbit polyclonal antibody (STJ24066) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HNRNPU antibody (608-825) (STJ24066)

SKU:
STJ24066

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HNRNPU (608-825) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNRNPU
Gene ID: 3192
Uniprot ID: HNRPU_HUMAN
Immunogen Region: 608-825
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 608-825 of human hnRNP U (NP_114032.2).
Immunogen Sequence: PKDEDYKQRTQKKAEVEGKD LPEHAVLKMKGNFTLPEVAE CFDEITYVELQKEEAQKLLE QYKEESKKALPPEKKQNTGS KKSNKNKSGKNQFNRGGGHR GRGGFNMRGGNFRGGAPGNR GGYNRRGNMPQRGGGGGGSG GIGYPYPRAPVFPGRGSYSN RGNYNRGGMPNRGNYNQNFR GRGNNRGYKNQSQGYNQWQQ GQFWGQKPWSQHYHQGYY
Tissue Specificity Widely expressed.
Post Translational Modifications Cleaved at Asp-100 by CASP3 during T-cell apoptosis, resulting in a loss of DNA- and chromatin-binding activities. Extensively phosphorylated. Phosphorylated on Ser-59 by PLK1 and dephosphorylated by protein phosphatase 2A (PP2A) in mitosis. Arg-739 is dimethylated, probably to asymmetric dimethylarginine (Ref.8). Arg-733 is dimethylated, probably to asymmetric dimethylarginine. Citrullinated by PADI4.
Function DNA- and RNA-binding protein involved in several cellular processes such as nuclear chromatin organization, telomere-length regulation, transcription, mRNA alternative splicing and stability, Xist-mediated transcriptional silencing and mitotic cell progression. Plays a role in the regulation of interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs) in a transcription-dependent manner, and thereby maintains genomic stability. Required for the localization of the long non-coding Xist RNA on the inactive chromosome X (Xi) and the subsequent initiation and maintenance of X-linked transcriptional gene silencing during X-inactivation. Plays a role as a RNA polymerase II (Pol II) holoenzyme transcription regulator. Promotes transcription initiation by direct association with the core-TFIIH basal transcription factor complex for the assembly of a functional pre-initiation complex with Pol II in a actin-dependent manner. Blocks Pol II transcription elongation activity by inhibiting the C-terminal domain (CTD) phosphorylation of Pol II and dissociates from Pol II pre-initiation complex prior to productive transcription elongation. Positively regulates CBX5-induced transcriptional gene silencing and retention of CBX5 in the nucleus. Negatively regulates glucocorticoid-mediated transcriptional activation. Key regulator of transcription initiation and elongation in embryonic stem cells upon leukemia inhibitory factor (LIF) signaling. Involved in the long non-coding RNA H19-mediated Pol II transcriptional repression. Participates in the circadian regulation of the core clock component BMAL1 transcription. Plays a role in the regulation of telomere length. Plays a role as a global pre-mRNA alternative splicing modulator by regulating U2 small nuclear ribonucleoprotein (snRNP) biogenesis. Plays a role in mRNA stability. Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Enhances the expression of specific genes, such as tumor necrosis factor TNFA, by regulating mRNA stability, possibly through binding to the 3'-untranslated region (UTR). Plays a role in mitotic cell cycle regulation. Involved in the formation of stable mitotic spindle microtubules (MTs) attachment to kinetochore, spindle organization and chromosome congression. Phosphorylation at Ser-59 by PLK1 is required for chromosome alignement and segregation and progression through mitosis. Contributes also to the targeting of AURKA to mitotic spindle MTs. Binds to double- and single-stranded DNA and RNA, poly(A), poly(C) and poly(G) oligoribonucleotides. Binds to chromatin-associated RNAs (caRNAs). Associates with chromatin to scaffold/matrix attachment region (S/MAR) elements in a chromatin-associated RNAs (caRNAs)-dependent manner. Binds to the Xist RNA. Binds the long non-coding H19 RNA. Binds to SMN1/2 pre-mRNAs at G/U-rich regions. Binds to small nuclear RNAs (snRNAs). Binds to the 3'-UTR of TNFA mRNA. Binds (via RNA-binding RGG-box region) to the long non-coding Xist RNA.this binding is direct and bridges the Xist RNA and the inactive chromosome X (Xi). Also negatively regulates embryonic stem cell differentiation upon LIF signaling. Required for embryonic development. Binds to brown fat long non-coding RNA 1 (Blnc1).facilitates the recruitment of Blnc1 by ZBTB7B required to drive brown and beige fat development and thermogenesis. (Microbial infection) Negatively regulates immunodeficiency virus type 1 (HIV-1) replication by preventing the accumulation of viral mRNA transcripts in the cytoplasm.
Protein Name Heterogeneous Nuclear Ribonucleoprotein U
Hnrnp U
Grip120
Nuclear P120 Ribonucleoprotein
Scaffold-Attachment Factor A
Saf-A
P120
Pp120
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72203
Cellular Localisation Nucleus
Nucleus Matrix
Chromosome
Nucleus Speckle
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centromere
Kinetochore
Spindle
Spindle Pole
Midbody
Cell Surface
Cytoplasmic Granule
Localizes At Inactive X Chromosome (Xi) Regions
Localizes In The Nucleus During Interphase
At Metaphase
Localizes With Mitotic Spindle Microtubules (Mts)
At Anaphase
Localizes In The Mitotic Spindle Midzone
Localizes In Spindle Mts Proximal To Spindle Poles In A Tpx2- And Aurka-Dependent Manner
The Ser-59 Phosphorylated Form Localizes To Centrosomes During Prophase And Metaphase
To Mitotic Spindles In Anaphase And To The Midbody During Cytokinesis
Colocalizes With Smarca4 In The Nucleus
Colocalizes With Cbx5 In The Nucleus
Colocalizes With Nr3c1 In Nuclear Speckles
Localized In Cytoplasmic Ribonucleoprotein (Rnp) Granules Containing Untranslated Mrnas
Alternative Antibody Names Anti-Heterogeneous Nuclear Ribonucleoprotein U antibody
Anti-Hnrnp U antibody
Anti-Grip120 antibody
Anti-Nuclear P120 Ribonucleoprotein antibody
Anti-Scaffold-Attachment Factor A antibody
Anti-Saf-A antibody
Anti-P120 antibody
Anti-Pp120 antibody
Anti-HNRNPU antibody
Anti-C1orf199 antibody
Anti-HNRPU antibody
Anti-SAFA antibody
Anti-U21.1 antibody

Information sourced from Uniprot.org

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