Developmental Biology | Research Area | St John's Laboratory

Western blot analysis of various lysates using [KO Validated] HIRA Rabbit pAb (STJ110759) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-HIRA antibody (250-550) (STJ110759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using HIRA Antibody (STJ114401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HIRA antibody (250-550) (STJ114401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using HIRA Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-HIRA antibody (521-570 aa) (STJ93503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using HIRA Rabbit mAb (STJ11103638) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-HIRA antibody (550-675) [S8MR] (STJ11103638)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using Histone H2AX antibody (STJ29383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Histone H2A antibody (1-143) (STJ29383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Western blot analysis of various lysates using Histone H2AX Rabbit pAb (STJ113097) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Histone H2A antibody (50-143) (STJ113097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Anti-Histone H2AX antibody (1-143 aa) (STJ11106040)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Anti-Histone H2B antibody [SAA1806] (STJA0020234)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and Histone H3. 3 rabbit monoclonal antibody (STJ11102163). The ChIP sequencing results indicate the enrichment pattern of Histone H3. 3 in selected genomic region and representative gene loci (GAPDH) , as shown in figure.

Anti-Histone H3 antibody (57-136) [S3MR] (STJ11102163)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Anti-Histone H3 antibody [SM0412] (STJA0010412)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Histone H3.2 antibody (81-131 aa) (STJ194163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using [KO Validated] Histone H4 Rabbit pAb (STJ11106815) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Histone H4 antibody (1-100 aa) (STJ11106815)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Immunofluorescence analysis of NIH/3T3 cells using Histone H4 Rabbit polyclonal antibody (STJ119273) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Histone H4 antibody (1-100) (STJ119273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Western blot analysis of various lysates, using Histone H4 Rabbit polyclonal antibody (STJ29382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Histone H4 antibody (1-100) (STJ29382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Immunohistochemistry analysis of Histone H4 in paraffin-embedded mouse kidney using Histone H4 Rabbit monoclonal antibody (STJ11103155) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-Histone H4 antibody (24-103) [S5MR] (STJ11103155)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Immunocytochemistry analysis of Histone H4 (green) in Hela using Histone H4 antibody, and DAPI (blue).

Anti-Histone H4 antibody (51-70 aa) [R05-8H9] (STJA0011428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/ChIP

Reactivity :

Human

£246.50

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Immunofluorescence analysis of human-liver tissue. 1, Histone H4 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Histone H4 antibody (6-55 aa) (STJ93539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HJV antibody (270-350 aa) (STJ192361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-HJV/Hemojuvelin antibody [SAA1409] (STJA0027752)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human/Rat

£254.50 - £378.50

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Anti-HJV/Hemojuvelin antibody [SAA1409] {APC} (STJA0029614)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human/Rat

£310.50 - £472.50

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Anti-HJV/Hemojuvelin antibody [SAA1409] {FITC} (STJA0028648)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human/Rat

£254.50 - £378.50

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Anti-HJV/Hemojuvelin antibody [SAA1409] {PerCP} (STJA0030580)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human/Rat

£292.50 - £440.50

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Anti-HJV/Hemojuvelin antibody [SAA1409] {PE} (STJA0031546)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human/Rat

£310.50 - £472.50

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Immunofluorescence analysis of paraffin-embedded Mouse liver tissue using HMGCR Rabbit polyclonal antibody (STJ11105656) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-HMGCR antibody (200-300) (STJ11105656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using HMGCR Rabbit polyclonal antibody (STJ119212) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HMGCR antibody (351-440) (STJ119212)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using HMGCR Rabbit monoclonal antibody (STJ11101601) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HMGCR antibody (400-500) [S1MR] (STJ11101601)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of paraffin-embedded Human liver tissue using HMGCR Rabbit polyclonal antibody (STJ115278) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-HMGCR antibody (629-888) (STJ115278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using HMGCR Rabbit polyclonal antibody (STJ116941) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HMGCR antibody (629-888) (STJ116941)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using (STJ119211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HMGCR antibody (783-791) (STJ119211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of Rat brain, using HMGCR rabbit polyclonal antibody (STJ24046) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HMGCR antibody (783-791) (STJ24046)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HMGCR antibody (STJA0006761)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using HNF-6/ONECUT1 Rabbit mAb (STJ11107325) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-HNF-6/ONECUT1 antibody (167-292 aa) [SRM] (STJ11107325)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using HNF1 Alpha antibody (STJ24056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-HNF1A antibody (30-130) (STJ24056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HNF1A antibody (STJ193036)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts from Jurkat cells, using HNF-1 Beta Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-HNF1B antibody (N-Term) (STJ96448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of SW480 cells, using HNF4A antibody (STJ24060) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HNF4A antibody (226-325) (STJ24060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using HNF-4 Alpha Polyclonal Antibody diluted at 1：2000

Anti-HNF4A antibody (280-329 aa) (STJ93554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of mouse large intestine, using HNF4G antibody (STJ118492) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-HNF4G antibody (366-445) (STJ118492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HNF4G antibody (STJA0007279)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HeLa, COLO, and 293 cells, using HOXA1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-HOXA1 antibody (171-220 aa) (STJ93573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells using HOXA11 antibody (STJ24071) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HOXA11 antibody (1-180) (STJ24071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HOXA11 antibody (STJ190879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HOXA2 antibody (160-240 aa) (STJ190183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-HOXA3 antibody (254-443 aa) (STJ11107486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using HoxA6 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-HOXA6 antibody (101-150 aa) (STJ93577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Mouse lung, using HOXA6 Rabbit pAb (STJ11102754) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-HOXA6 antibody (30-130) (STJ11102754)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of U-2 OS cells using HOXB1 antibody (STJ28702) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HOXB1 antibody (35-180) (STJ28702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HOXB1 antibody (STJ190881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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