Developmental Biology | Research Area | St John's Laboratory

Whole cell lysates of K562 were separated by 10% SDS-PAGE, and the membrane was blotted with anti-TGF β1 (PT2173) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562Predicted band size: 44kDaObserved band size: 44kDa

Anti-TGFB1 antibody (300-390) [PT2173] (STJA0006239)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human Endometrium. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-TGFB1 antibody (336-385 aa) (STJ95997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-TGFB1 antibody [1F12] (STJ196644)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Western blot analysis of K-562, using TGFbeta1 antibody (STJ11104240) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TGFB1 antibody [S4240RM] (STJ11104240)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Anti-TGFB1/TGF-beta-1 antibody [SAA0180] (STJA0026960)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£315.50

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Anti-TGFB1/TGF-beta-1 antibody [SAA0180] {APC} (STJA0028938)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£391.50

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Anti-TGFB1/TGF-beta-1 antibody [SAA0180] {FITC} (STJA0027972)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£315.50

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Anti-TGFB1/TGF-beta-1 antibody [SAA0180] {PerCP} (STJA0029904)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£365.50

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Anti-TGFB1/TGF-beta-1 antibody [SAA0180] {PE} (STJA0030870)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£391.50

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Anti-TGFB1/TGF-beta-1 antibody [SAA0443] (STJA0027620)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£286.50

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Anti-TGFB1/TGF-beta-1 antibody [SAA0443] {APC} (STJA0028939)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Anti-TGFB1/TGF-beta-1 antibody [SAA0443] {FITC} (STJA0027973)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£284.50

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Anti-TGFB1/TGF-beta-1 antibody [SAA0443] {PerCP} (STJA0029905)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£330.50

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Anti-TGFB1/TGF-beta-1 antibody [SAA0443] {PE} (STJA0030871)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£352.50

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Western blot analysis of extracts of various cell lines, using TGM1 antibody (STJ115322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TGM1 antibody (100-280) (STJ115322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-TGM1 antibody (STJA0006872)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TGM5 antibody (448-498 aa) (STJ195620)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of TGM5 in paraffin-embedded mouse heart using TGM5 Rabbit polyclonal antibody (STJ29119) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-TGM5 antibody (461-720) (STJ29119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using TGS1 Rabbit pAb (STJ110158) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-TGS1 antibody (714-853) (STJ110158)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-TGS1 antibody (STJA0009384)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of Hela lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-THRAP3 antibody (110-190 aa) (STJ190266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HeLa cells, using THRAP3 Rabbit pAb (STJ111686) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-THRAP3 antibody (340-500) (STJ111686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-TIAM 1 antibody (STJ13100389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC

Reactivity :

Human/Mouse

£322.50

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Anti-TIAM 1 antibody (STJ13100390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC

Reactivity :

Human/Mouse

£322.50

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using TIAM1 antibody (STJ112290) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-TIAM1 antibody (1392-1591) (STJ112290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Anti-TIAM1 antibody (STJ192977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of human colon carcinoma using TLN1 antibody (STJ25860) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-TLN1 antibody (1-220) (STJ25860)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western Blot analysis of K562 cells using Talin-1 Polyclonal Antibody. The antibody was diluted at 1:1000. The STJ97271

Anti-TLN1 antibody (2471-2520 aa) (STJ97271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-TNF alpha antibody (100-150) (STJ506267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£400.50

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Anti-TNF alpha antibody (100-150) {Biotin} (STJ506268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£497.50

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Anti-TNF alpha antibody (100-150) {FITC} (STJ506269)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£497.50

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TNF-alpha antibody standard curve mouse monoclonal STJA0032443

Anti-TNF alpha antibody [5F3-D10-F11] (STJA0032443)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£133.50 - £281.50

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TNF alpha antibody western blot recombinant human protein STJA0032876

Anti-TNF alpha antibody [6H2-4C6-5B6] (STJA0032876)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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TNF alpha antibody western blot Recombinant Human TNF alpha STJA0032958

Anti-TNF alpha antibody [9D9-6A9-8B4] (STJA0032958)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Western blot analysis of THP-1, using TNF-Alpha rabbit polyclonal antibody (STJ25882) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight and LPS (1 Mu g/ml) at 37 °C for 6 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-TNF antibody (134-233) (STJ25882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of human-stomach tissue. 1, TNF-Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-TNF antibody (141-190 aa) (STJ96054)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Human mammary cancer cells using TNF-Alpha antibody (STJ113176) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-TNF antibody (77-233) (STJ113176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human lung using TNF-Alpha Rabbit monoclonal antibody (STJ11104248) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-TNF antibody (77-233) [S4248RM] (STJ11104248)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various lysates, using TNF-Alpha antibody (STJ11104757) at1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/ml) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TNF antibody (77-233) [S4757RM] (STJ11104757)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti TNF Goat Polyclonal antibody western blot STJ140123

Anti-TNF antibody (STJ140123)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

£135.50 - £440.50

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Western blot analysis of Recombinant Human TNF a Protein with TNF Alpha Mouse mAb at 1:2, 000.

Anti-TNF antibody [Q34] (STJ97493)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-TNF-Alpha antibody (STJ16101171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-F/FC/ELISA/IHC-P/WB

Reactivity :

Mouse/Rat

£757.50

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Anti-TNF-alpha antibody [EBS-O-174] (STJ16102602)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB/IF/IHC

Reactivity :

Human/Rat

£308.50 - £1,349.50

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Anti-TNF-alpha antibody [EBS-O-175] (STJ16102603)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB/IF/IHC

Reactivity :

Human

£308.50 - £1,349.50

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Anti-TNF-Alpha antibody [T1] (STJ16100934)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/ELISA/WB

Reactivity :

Human

£586.50

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Anti-TNF-Alpha antibody [T3] (STJ16100932)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/ELISA/IP/WB

Reactivity :

Human

£586.50

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Anti-TNF-Alpha antibody [V1q] (STJ16100926)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Mouse

£713.50

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Anti-TNF-Alpha antibody {PE-Cy7} (STJ11109125)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

£215.50 - £628.50

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