Anti-TLN1 antibody (1-220) (STJ25860)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ25860
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General Information

Short DescriptionRabbit polyclonal TLN1 (1-220) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolTLN1
Gene ID7094
Uniprot IDTLN1_HUMAN
Immunogen Region1-220
Immunogen SequenceMVALSLKISIGNVVKTMQFE PSTMVYDACRIIRERIPEAP AGPPSDFGLFLSDDDPKKGI WLEAGKALDYYMLRNGDTME YRKKQRPLKIRMLDGTVKTI MVDDSKTVTDMLMTICARIG ITNHDEYSLVRELMEEKKEE ITGTLRKDKTLLRDEKKMEK LKQKLHTDDELNWLDHGRTL REQGVEEHETLLLRRKFFYS DQNVDSRDPVQLNLLYVQAR
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLN1 (NP_006280.3).

Additional Info

Tissue Specificity Isoform 2: Expressed at low to non-detectable levels in many tissues but highly expressed in skin and pancreas with other tissues including kidney cortex, endocervix, testis, pituitary, liver, and spleen also showing robust expression.
Function High molecular weight cytoskeletal protein concentrated at regions of cell-matrix and cell-cell contacts. Involved in connections of major cytoskeletal structures to the plasma membrane. With KANK1 co-organize the assembly of cortical microtubule stabilizing complexes (CMSCs) positioned to control microtubule-actin crosstalk at focal adhesions (FAs) rims.
Protein Name Talin-1
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-354192
Reactome: R-HSA-354194
Reactome: R-HSA-372708
Reactome: R-HSA-381038
Reactome: R-HSA-399955
Reactome: R-HSA-445355
Reactome: R-HSA-5674135
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9856530
Cellular Localisation Cell Projection
Ruffle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Cell Surface
Cell Junction
Focal Adhesion
Colocalizes With Layn At The Membrane Ruffles
Localized Preferentially In Focal Adhesions Than Fibrillar Adhesions
Alternative Antibody Names Anti-Talin-1 antibody
Anti-TLN1 antibody
Anti-KIAA1027 antibody
Anti-TLN antibody

Information sourced from Uniprot.org

Citations

Product Review