• Immunohistochemistry analysis of TGM5 in paraffin-embedded mouse heart using TGM5 Rabbit polyclonal antibody (STJ29119) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TGM5 in paraffin-embedded human colon using TGM5 Rabbit polyclonal antibody (STJ29119) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TGM5 in paraffin-embedded rat kidney using TGM5 Rabbit polyclonal antibody (STJ29119) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and 293T cells transfected with TGM5 using TGM5 Rabbit polyclonal antibody (STJ29119) at 1:7000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TGM5 antibody (461-720) (STJ29119)

SKU:
STJ29119

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TGM5 (461-720) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:8000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TGM5
Gene ID: 9333
Uniprot ID: TGM5_HUMAN
Immunogen Region: 461-720
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 461-720 of human TGM5 (NP_963925.2).
Immunogen Sequence: LQKLKARSFHGSQRGAELQP SRPTSLSQDSPRSLHTPSLR PSDVVQVSLKFKLLDPPNMG QDICFVLLALNMSSQFKDLK VNLSAQSLLHDGSPLSPFWQ DTAFITLSPKEAKTYPCKIS YSQYSQYLSTDKLIRISALG EEKSSPEKILVNKIITLSYP SITINVLGAAVVNQPLSIQV IFSNPLSEQVEDCVLTVEGS GLFKKQQKVFLGVLKPQHQA SIILETVPFKSGQRQIQAN
Tissue Specificity Expressed in foreskin keratinocytes.
Function Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Contributes to the formation of the cornified cell envelope of keratinocytes.
Protein Name Protein-Glutamine Gamma-Glutamyltransferase 5
Transglutaminase X
Tg(X
Tgx
Tgase X
Transglutaminase-5
Tgase-5
Database Links Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Associated With Intermediate Filaments
Alternative Antibody Names Anti-Protein-Glutamine Gamma-Glutamyltransferase 5 antibody
Anti-Transglutaminase X antibody
Anti-Tg(X antibody
Anti-Tgx antibody
Anti-Tgase X antibody
Anti-Transglutaminase-5 antibody
Anti-Tgase-5 antibody
Anti-TGM5 antibody
Anti-TGMX antibody

Information sourced from Uniprot.org

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