Anti-THRAP3 antibody (340-500) (STJ111686)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal THRAP3 (340-500) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | THRAP3 |
| Gene ID | 9967 |
| Uniprot ID | TR150_HUMAN |
| Immunogen Region | 340-500 |
| Immunogen Sequence | GGAAYTKRYLEEQKTENGKD KEQKQTNTDKEKIKEKGSFS DTGLGDGKMKSDSFAPKTDS EKPFRGSQSPKRYKLRDDFE KKMADFHKEEMDDQDKDKAK GRKESEFDDEPKFMSKVIGA NKNQEEEKSGKWEGLVYAPP GKEKQRKTEELEEESFPERS K |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 340-500 of human THRAP3 (NP_005110.2). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | ADP-ribosylation during genotoxic stress promotes accumulation in nuclear speckles. |
| Function | Involved in pre-mRNA splicing. Remains associated with spliced mRNA after splicing which probably involves interactions with the exon junction complex (EJC). Can trigger mRNA decay which seems to be independent of nonsense-mediated decay involving premature stop codons (PTC) recognition. May be involved in nuclear mRNA decay. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45 is proposed to sequester phosphorylated SFPQ from PTPRC/CD45 pre-mRNA in resting T-cells. Involved in cyclin-D1/CCND1 mRNA stability probably by acting as component of the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Involved in response to DNA damage. Is excluced from DNA damage sites in a manner that parallels transcription inhibition.the function may involve the SNARP complex. Initially thought to play a role in transcriptional coactivation through its association with the TRAP complex.however, it is not regarded as a stable Mediator complex subunit. Cooperatively with HELZ2, enhances the transcriptional activation mediated by PPARG, maybe through the stabilization of the PPARG binding to DNA in presence of ligand. May play a role in the terminal stage of adipocyte differentiation. Plays a role in the positive regulation of the circadian clock. Acts as a coactivator of the CLOCK-BMAL1 heterodimer and promotes its transcriptional activator activity and binding to circadian target genes. |
| Protein Name | Thyroid Hormone Receptor-Associated Protein 3Bclaf1 And Thrap3 Family Member 2Thyroid Hormone Receptor-Associated Protein Complex 150 Kda ComponentTrap150 |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-381340 |
| Cellular Localisation | NucleusNucleoplasmNucleus Speckle |
| Alternative Antibody Names | Anti-Thyroid Hormone Receptor-Associated Protein 3 antibodyAnti-Bclaf1 And Thrap3 Family Member 2 antibodyAnti-Thyroid Hormone Receptor-Associated Protein Complex 150 Kda Component antibodyAnti-Trap150 antibodyAnti-THRAP3 antibodyAnti-BCLAF2 antibodyAnti-TRAP150 antibody |
Information sourced from Uniprot.org