Autophagy | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using eEF1A1 antibody (STJ119870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EEF1A1 antibody (301-400) (STJ119870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Immunocytochemistry analysis of Elongation Factor 1A1 (green) in Hela using Elongation Factor 1A1 antibody, and DAPI (blue).

Anti-EEF1A1 antibody (325-338 aa) [R04-3H4] (STJA0012354)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

£264.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded Human tonsil using eEF1A1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-EEF1A1 antibody (434-447 aa) [R05-4K5] (STJA0011256)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£264.50

Choose Options

Anti-eEF1A1 antibody [SRM14654] (STJ11109384)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

Add to Cart The item has been added

Western blot analysis of various lysates using EPAS1/HIF2 Alpha Rabbit pAb (STJ29691) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-EPAS1 antibody (678-745) (STJ29691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Immunofluorescence analysis of human-stomach tissue. 1, EPAS-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-EPAS1 antibody (STJ96690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunohistochemistry analysis of FUNDC1 in paraffin-embedded Mouse brain tissue using FUNDC1 Rabbit polyclonal antibody (STJ118748) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-FUNDC1 antibody (1-155) (STJ118748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

Choose Options

Western blot analysis of AD293 cells using FUNDC1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-FUNDC1 antibody (1-50 aa) (STJ97353)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of extracts of K-562 cells, using FUNDC1 antibody (STJ11104646) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-FUNDC1 antibody (50-155) (STJ11104646)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

Choose Options

Anti-FUNDC1 antibody [SRM13549] (STJ11108279)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

Add to Cart The item has been added

Immunohistochemistry analysis of paraffin-embedded mouse kidney using GABARAP antibody (STJ114442) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-GABARAP antibody (1-100) (STJ114442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Immunofluorescence analysis of HeLa cells using GABARAP antibody (STJ115794).

Anti-GABARAP antibody (1-100) (STJ115794)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of extracts of various cell lines, using GABARAP Rabbit monoclonal antibody (STJ11102043) at 1:1000 dilution. 293T cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GABARAP antibody (1-100) [S3MR] (STJ11102043)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

Choose Options

Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GABARAP antibody (8-58 aa) (STJ194630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Anti-GABARAP antibody (N-Term) (STJ13101124)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

Choose Options

Anti-GABARAP antibody (N-Term) (STJ13101168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

Choose Options

Anti-GABARAP antibody (N-Term) (STJA0005686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£488.50

Choose Options

Anti-GABARAP antibody (STJ13101123)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

Choose Options

Western blot analysis of various lysates using GABARAPL1 Rabbit mAb (STJ11107315) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 30s.

Anti-GABARAPL1 antibody (1-100 aa) [SRM5404] (STJ11107315)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

Choose Options

Western blot analysis of Mouse brain, using GABARAPL1 antibody (STJ110100) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-GABARAPL1 antibody (1-50) (STJ110100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Anti-GABARAPL1 antibody (32-82) (STJA0006650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Anti-GABARAPL1 antibody [SRM14682] (STJ11109412)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

Add to Cart The item has been added

Western blot analysis of various lysates using GABARAPL2 Rabbit polyclonal antibody (STJ117055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GABARAPL2 antibody (1-50) (STJ117055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

Choose Options

Western blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-GABARAPL2 antibody (26-75 aa) (STJ98595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of various lysates using GABARAPL2 Rabbit mAb (STJ11102498) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-GABARAPL2 antibody (38-117) [S8MR] (STJ11102498)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

Choose Options

Anti-Gamma-aminobutyric acid receptor-associated protein antibody (N-Term) (STJA0040908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-F/WB

Reactivity :

Human/Rat

£528.50

Choose Options

Anti-Gamma-aminobutyric acid receptor-associated protein-like 2 antibody (N-Term) (STJA0040907)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-F/WB

Reactivity :

Human/Rat

£528.50

Choose Options

Western blot analysis of extracts of various cell lines, using GFAP antibody (STJ11101596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GFAP antibody (1-100) [S6MR] (STJ11101596)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

Choose Options

Western blot analysis of mouse brain, using GFAP rabbit polyclonal antibody (STJ23770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GFAP antibody (1-75) (STJ23770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Immunofluorescence analysis of Hela cell. 1, GFAP Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-GFAP antibody (11-60 aa) (STJ93259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Formalin-fixed, paraffin-embedded human brain stained with anti-GFAP antibody using peroxidase-conjugate and DAB chromogen. Note positive staining of astrocytic processes

Anti-GFAP antibody (150-250aa) [ZR356] (STJ180514)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£161.50 - £572.50

Choose Options

Anti-GFAP antibody (250-300 aa) (STJ13100497)

Clonality :

Polyclonal

Host :

Guinea Pig

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

£322.50

Choose Options

Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-GFAP (ABT176) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Neuro-2aPredicted band size: 50kDaObserved band size: 57kDa

Anti-GFAP antibody (300-432 aa) [ABT176] (STJ197157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£180.00 - £261.00

Choose Options

Human astrocytoma tissue was stained with Anti-Glial Fibrillary Acidic Protein (GFAP) (ABT470) Antibody

Anti-GFAP antibody (300-432 aa) [ABT470] (STJ190068)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

Choose Options

Immunofluorescence analysis of U-251MG cells using GFAP Rabbit polyclonal antibody (STJ116876) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-GFAP antibody (350-432) (STJ116876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Anti-GFAP antibody at 1:2500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-GFAP antibody (375aa C-Term) (STJ140132)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey

£135.50 - £394.50

Choose Options

Immunohistochemistry of mouse brain with Anti-GFAP primary antibody (STJ501145) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-GFAP antibody (50-100) (STJ501145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

Choose Options

Anti-GFAP antibody (50-100) {Biotin} (STJ501146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

Choose Options

Anti-GFAP antibody (50-100) {FITC} (STJ501147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

Choose Options

Anti-GFAP antibody (STJ16102773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-F/IHC-P/WB

Reactivity :

Bovine/Human/Rat

£514.50

Choose Options

Anti-GFAP antibody [3F2] (STJ16103424)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£746.50

Choose Options

Immunofluorescence analysis of Hela cell. 1, AR Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GFAP monoclonal antibody (5C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-GFAP antibody [5C8] (STJ96961)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Anti-GFAP antibody [EP672Y] (STJ16100372)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£658.50 - £1,217.50

Choose Options

Human brain stained with anti-GFAP antibody using peroxidase-conjugate and DAB chromogen. Note positive staining of astrocytic processes.

Anti-GFAP antibody [GA‐5] (STJ180110)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £572.50

Choose Options

Anti-GFAP antibody [GF-02] (STJ16103141)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IHC-F/ICC

Reactivity :

Human/Pig

£504.50

Choose Options

Anti-GFAP antibody [H8/E9] (STJA0019392)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£209.50 - £1,333.50

Choose Options

Anti-GFAP antibody [N206B/9R] (STJA0019391)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC/WB

Reactivity :

Human

£209.50 - £1,333.50

Choose Options

Anti-GFAP antibody [PT0298R] (STJA0039609)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

Choose Options