| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-DYNC1I2 (352-612) is suitable for use in Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DYNC1I2 |
| Gene ID: | 1781 |
| Uniprot ID: | DC1I2_HUMAN |
| Immunogen Region: | 352-612 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 352-612 of human DYNC1I2 (NP_001258717.1). |
| Immunogen Sequence: | AAHTHPVYCVNVVGTQNAHN LISISTDGKICSWSLDMLSH PQDSMELVHKQSKAVAVTSM SFPVGDVNNFVVGSEEGSVY TACRHGSKAGISEMFEGHQG PITGIHCHAAVGAVDFSHLF VTSSFDWTVKLWTTKNNKPL YSFEDNADYVYDVMWSPTHP ALFACVDGMGRLDLWNLNND TEVPTASISVEGNPALNRVR WTHSGREIAVGDSEGQIVIY DVGEQIAVPRNDEWARFGR |
| Post Translational Modifications | The phosphorylation status of Ser-90 appears to be involved in dynactin-dependent target binding. Pyrophosphorylation by 5-diphosphoinositol pentakisphosphate (5-IP7) promotes interaction with DCTN1. Serine pyrophosphorylation is achieved by Mg(2+)-dependent, but enzyme independent transfer of a beta-phosphate from a inositol pyrophosphate to a pre-phosphorylated serine residue. |
| Function | Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCTN1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes. |
| Protein Name | Cytoplasmic Dynein 1 Intermediate Chain 2Cytoplasmic Dynein Intermediate Chain 2Dynein Intermediate Chain 2 - CytosolicDh Ic-2 |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2132295Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-2565942Reactome: R-HSA-3371497Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-5663220Reactome: R-HSA-6807878Reactome: R-HSA-6811436Reactome: R-HSA-68877Reactome: R-HSA-8854518Reactome: R-HSA-9609690Reactome: R-HSA-9646399Reactome: R-HSA-9648025 |
| Cellular Localisation | CytoplasmCytoskeletonDetected In The Cytoplasm Of Pachytene SpermatocytesLocalizes To The Manchette In Elongating Spermatids |
| Alternative Antibody Names | Anti-Cytoplasmic Dynein 1 Intermediate Chain 2 antibodyAnti-Cytoplasmic Dynein Intermediate Chain 2 antibodyAnti-Dynein Intermediate Chain 2 - Cytosolic antibodyAnti-Dh Ic-2 antibodyAnti-DYNC1I2 antibodyAnti-DNCI2 antibodyAnti-DNCIC2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and DNA Ligase IV knockout (KO) HeLa cells, using [KO Validated] DNA Ligase IV Rabbit polyclonal antibody (STJ11100023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89095/357554/STJ11100023_1__39011.1713121676.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DNA Ligase IV knockout (KO) HeLa cells, using [KO Validated] DNA Ligase IV Rabbit polyclonal antibody (STJ11100023) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")