• Immunohistochemistry analysis of paraffin-embedded mouse testis using CHMP2B Rabbit monoclonal antibody (STJ11102976) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CHMP2B antibody (STJ11102976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CHMP2B antibody (114-213) [S6MR] (STJ11102976)

SKU:
STJ11102976

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CHMP2B (114-213) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHMP2B
Gene ID: 25978
Uniprot ID: CHM2B_HUMAN
Immunogen Region: 114-213
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 114-213 of human CHMP2B (Q9UQN3).
Immunogen Sequence: KKMDPQKTLQTMQNFQKENM KMEMTEEMINDTLDDIFDGS DDEEESQDIVNQVLDEIGIE ISGKMAKAPSAARSLPSAST SKATISDEEIERQLKALGVD
Tissue Specificity Widely expressed. Expressed in brain, heart, skeletal muscle, spleen, kidney, liver, small intestine, pancreas, lung, placenta and leukocytes. In brain, it is expressed in cerebellum, cerebral cortex, medulla, spinal chord, occipital lobe, frontal lobe, temporal lobe and putamen.
Function Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.
Protein Name Charged Multivesicular Body Protein 2b
Chmp2.5
Chromatin-Modifying Protein 2b
Chmp2b
Vacuolar Protein Sorting-Associated Protein 2-2
Vps2-2
Hvps2-2
Database Links Reactome: R-HSA-162588
Reactome: R-HSA-1632852
Reactome: R-HSA-5620971
Reactome: R-HSA-917729
Reactome: R-HSA-9610379
Reactome: R-HSA-9615710
Reactome: R-HSA-9668328
Reactome: R-HSA-9679504
Reactome: R-HSA-9694676
Cellular Localisation Cytoplasm
Cytosol
Late Endosome Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Charged Multivesicular Body Protein 2b antibody
Anti-Chmp2.5 antibody
Anti-Chromatin-Modifying Protein 2b antibody
Anti-Chmp2b antibody
Anti-Vacuolar Protein Sorting-Associated Protein 2-2 antibody
Anti-Vps2-2 antibody
Anti-Hvps2-2 antibody
Anti-CHMP2B antibody
Anti-CGI-84 antibody

Information sourced from Uniprot.org

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