Autophagy | Research Area | St John's Laboratory

Western blot analysis of A549 cells using Phospho-Vimentin (S56) Polyclonal Antibody

Anti-Phospho-VIM-Ser56 antibody (31-80 aa) (STJ90927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of 293 cells using Phospho-Vimentin (S83) Polyclonal Antibody

Anti-Phospho-VIM-Ser83 antibody (56-105 aa) (STJ90926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HELA cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000

Anti-Phospho-VIM-Tyr38 antibody (STJ98572)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysate from Jurkat cells, using phospho-Vimentin (Phospho-Tyr61) antibody.

Anti-Phospho-VIM-Tyr61 antibody (STJ90435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-Vimentin-S34 antibody [YT33] (STJA0019156)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-Phospho-Vimentin-S56 antibody [4A4] (STJA0019155)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-Phospho-Vimentin-S83 antibody [MO82] (STJA0019157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-Phospho-Vimentin-Ser39 antibody [R38-1F-8] (STJA0035697)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-Vimentin-Ser56 antibody [R01-0E2] (STJA0034994)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-Vimentin-Ser72 antibody [R04-1C8] (STJA0034995)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Rat

£133.50 - £281.50

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Anti-Phospho-Vimentin-Ser72 antibody [R37-4W-5] (STJA0035655)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-WIPI2-Ser413 antibody (STJ196473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-PI 3 Kinase p100 antibody [R02-7D7] (STJA0035882)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-PI 3 Kinase p150 antibody (STJA0037520)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£140.50 - £308.50

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Anti-PI 3 Kinase p150 antibody [R07-6N5] (STJA0037357)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunofluorescence analysis of NIH/3T3 cells using PIK3C3/VPS34 Rabbit polyclonal antibody (STJ114357) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-PIK3C3 antibody (130-412) (STJ114357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 600 Mu g extracts of Rat brain cells using 3 Mu g PIK3C3/VPS34 antibody (STJ11101401). Western blot was performed from the immunoprecipitate using PIK3C3/VPS34 antibody (STJ11101401) at a dilution of 1:500.

Anti-PIK3C3 antibody (788-887) [S1MR] (STJ11101401)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of 293T 3T3 cells using PI 3 Kinase Class 3 Polyclonal Antibody diluted at 1:1500. Secondary antibody was diluted at 1:20000

Anti-PIK3C3 antibody (STJ98590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of NIH/3T3 cells using PIK3R4/VPS15 Rabbit polyclonal antibody (STJ118273) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-PIK3R4 antibody (1-330) (STJ118273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PIK3R4 antibody (170-220) (STJA0006495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Confocal imaging of NIH/3T3 cells using PIK3R4/VPS15 Rabbit monoclonal antibody (STJ11103589, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PIK3R4/VPS15 antibody (1259-1358) [S9MR] (STJ11103589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-PIN/DYNLL1 antibody (1-89aa) [AT13C9] (STJA0041758)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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Western blot analysis of lysates from Rat skeletal muscle, using PINK1 Rabbit pAb (STJ29211) at 1:2000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-PINK1 antibody (282-581) (STJ29211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PINK1 antibody (Internal Region) (STJA0005787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£488.50

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Western Blot analysis of various cell lysis. primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJS000791 was diluted at 1:10000

Anti-PINK1 antibody (STJ192141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-PINK1 antibody (STJ502504)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

£400.50

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Anti-PINK1 antibody [PT0824R] (STJA0039772)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Anti-PINK1 antibody {Biotin} (STJ502505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

£497.50

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Anti-PINK1 antibody {FITC} (STJ502506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

£497.50

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Immunofluorescence analysis of C6 cells using Perilipin-2 Rabbit polyclonal antibody (STJ28198) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PLIN2 antibody (168-437) (STJ28198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western Blot analysis of varius cell lysis. primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJS000791 was diluted at 1:10000

Anti-PLIN2 antibody (STJ192987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using PLIN3 Rabbit polyclonal antibody (STJ116409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-PLIN3 antibody (135-434) (STJ116409)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PLIN3 antibody (STJ193144)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Polyubiqutin-B antibody [Ubi-1] (STJA0040757)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/IHC-P/WB

Reactivity :

Bovine/C.elegans/Chicken/Drosophila/Human

£528.50

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Immunofluorescence analysis of PC-12 cells using AMPK Alpha 1 Rabbit polyclonal antibody (STJ25112) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PRKAA1 antibody (1-300) (STJ25112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Mouse liver, using AMPK Alpha 1 Rabbit polyclonal antibody (STJ11104562) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PRKAA1 antibody (100-200) (STJ11104562)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£110.50 - £505.50

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Immunofluorescence analysis of rat-lung tissue. 1, AMPK Alpha 1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-PRKAA1 antibody (451-500 aa) (STJ91580)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using AMPK Alpha 1 monoclonal antibody against Jurkat (1) , HeLa (2) , HepG2 (3) , MCF-7 (4) , Cos7 (5) , NIH/3T3 (6) , K562 (7) , HEK293 (8) , and PC-12 (9) cell lysate.

Anti-PRKAA1 antibody [2B7] (STJ97826)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£149.00 - £360.00

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Western blot analysis of 1) Hela, 2) 293T, 3) 3T3, 4) PC12 with AMPK a1 Mouse mAb diluted at 1:2, 000.

Anti-PRKAA1 antibody [5G11] (STJ97538)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of PC-12 cells using AMPK Alpha 2 Rabbit polyclonal antibody (STJ11103813) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKAA2 antibody (270-343) (STJ11103813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of various lysates, using PRKAA2 antibody (STJ115987) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PRKAA2 antibody (270-552) (STJ115987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from wild type (WT) and AMPK Alpha 2 knockout (KO) HCT116 cells, using [KO Validated] AMPK Alpha 2 Rabbit polyclonal antibody (STJ29478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PRKAA2 antibody (270-552) (STJ29478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of 1) Hela, 2) 293T, 3) C2C12, 4) 3T3, 5) Rat Heart, 6) Rat Brain using AMPKα2 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-PRKAA2 antibody (STJ197442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of NIH-3T3 cells using AMPK Beta 1 Polyclonal Antibody (STJ114365) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKAB1 antibody (1-80) (STJ114365)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-PRKAB1 antibody (10-59 aa) (STJ91582)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of various lysates using AMPK Beta 1 Rabbit pAb (STJ110230) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PRKAB1 antibody (100-200) (STJ110230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of various cells using AMPK Beta 1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PRKAB1 antibody (147-196 aa) (STJ91583)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

Choose Options

Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PRKAB1 antibody (147-196 aa) (STJ91584)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options