Anti-PIK3R4/VPS15 antibody (1259-1358) [S9MR] (STJ11103589)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal PIK3R4/VPS15 (1259-1358) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S9MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PIK3R4 |
| Gene ID | 30849 |
| Uniprot ID | PI3R4_HUMAN |
| Immunogen Region | 1259-1358 |
| Immunogen Sequence | AGSDMKIRFWDLAYPERSYV VAGSTSSPSVSYYRKIIEGT EVVQEIQNKQKVGPSDDTPR RGPESLPVGHHDIITDVATF QTTQGFIVTASRDGIVKVWK |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1259-1358 of human PIK3R4/VPS15 (Q99570). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | Myristoylated. Probably autophosphorylated. |
| Function | Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2. |
| Protein Name | Phosphoinositide 3-Kinase Regulatory Subunit 4Pi3-Kinase Regulatory Subunit 4Pi3-Kinase P150 SubunitPhosphoinositide 3-Kinase Adaptor Protein |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1632852Reactome: R-HSA-1660514Reactome: R-HSA-1660516Reactome: R-HSA-1660517Reactome: R-HSA-168138Reactome: R-HSA-5668599Reactome: R-HSA-9679504Reactome: R-HSA-9694676Reactome: R-HSA-9705671Reactome: R-HSA-983170 |
| Cellular Localisation | Late EndosomeCytoplasmic VesicleAutophagosomeMembraneLipid-AnchorAs Component Of The Pi3k Complex I Localized To Pre-Autophagosome StructuresAs Component Of The Pi3k Complex Ii Localized Predominantly To EndosomesLocalizes Also To Discrete Punctae Along The Ciliary Axoneme |
| Alternative Antibody Names | Anti-Phosphoinositide 3-Kinase Regulatory Subunit 4 antibodyAnti-Pi3-Kinase Regulatory Subunit 4 antibodyAnti-Pi3-Kinase P150 Subunit antibodyAnti-Phosphoinositide 3-Kinase Adaptor Protein antibodyAnti-PIK3R4 antibodyAnti-VPS15 antibody |
Information sourced from Uniprot.org