Post Translational Modifications | Primary antibodies

Western blot analysis of various lysates using Phospho-VANGL2-T78/S79/S82 Rabbit mAb (STJ11103743) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by wnt (100ng/ml) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-VANGL2-T78/S79/S82 antibody [S3MR] (STJ11103743)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from HEL cells using Phospho-VASP-S157 Rabbit pAb (STJ116383) at 1:1000 dilution. HEL cells were treated by Forskolin (20 uM) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 0. 8s.

Anti-Phospho-VASP-S157 antibody (STJ116383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-VASP-S157 Rabbit monoclonal antibody (STJ11105906) at 1:4000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-VASP-S157 antibody [S5906RM] (STJ11105906)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from HEL cells, using Phospho-VASP-S157 Rabbit monoclonal antibody (STJ11105907) at 1:3000 dilution. HEL cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-VASP-S157 antibody [S5907RM] (STJ11105907)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from C6 cells, using Phospho-VASP-S239 Rabbit pAb (STJ22444) at 1:1000 dilution. C6 cells were treated by Forskolin (10 uM) at 37 °C for 1 hour after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-VASP-S239 antibody (STJ22444)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-VASP-S239 antibody [SRM13699] (STJ11108429)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various cells using Phospho-VASP (S157) Polyclonal Antibody

Anti-Phospho-VASP-Ser157 antibody (124-173 aa) (STJ90431)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human tonsil. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-VASP-Ser157 antibody (STJ90777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from RAW264.7 cells, using VASP (Phospho-Ser238) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-VASP-Ser238 antibody (206-255 aa) (STJ90432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-VASP-Ser239 antibody (STJ196466)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-VASP-Ser239 antibody [PT1281R] (STJA0040080)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-VASP-Thr-278 antibody (STJA0005634)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-VASP-Thr-278 antibody (STJA0041443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-VASP-Thr278 antibody (STJ91280)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-VAV1-Tyr160 antibody (131-180 aa) (STJ91129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from RAW264.7 cells, using VAV1 (Phospho-Tyr174) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-VAV1-Tyr174 antibody (141-190 aa) (STJ90434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-VAV1-Tyr174 antibody (STJ90433)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat cells, using Phospho-VAV1-Y174 Rabbit pAb (STJ11105770). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-VAV1-Y174 antibody (STJ11105770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£126.50 - £618.50

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Anti-Phospho-VAV2-Tyr-142 antibody (STJA0005636)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£367.50

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Anti-Phospho-Vav2-Tyr-142 antibody (STJA0041440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£542.50

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Anti-Phospho-VAV2-Tyr-172 antibody (STJA0005637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-Vav2-Tyr-172 antibody (STJA0041441)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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Immunofluorescence analysis of NIH/3T3 cells, using VAV2 (Phospho-Tyr142) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-VAV2-Tyr142 antibody (108-157 aa) (STJ91109)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of JK cells using Phospho-Vav3 (Y173) Polyclonal Antibody

Anti-Phospho-VAV3-Tyr173 antibody (STJ90999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vinculin (Phospho-Tyr821) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-VCL-Tyr821 antibody (786-835 aa) (STJ91311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 22RV1 using p-VCP (S352) antibody. Antibody was diluted at 1:1000

Anti-Phospho-VCP-Ser352 antibody (318-367 aa) (STJ90640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HT29 cells treated with heat shock, using Vitamin D Receptor (Phospho-Ser208) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-VDR-Ser208 antibody (181-230 aa) (STJ90730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of COLO cells using Phospho-VDR (S51) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-VDR-Ser51 antibody (16-65 aa) (STJ90600)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-VE-Cadherin-Tyr-685 antibody (STJA0041148)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£542.50

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Western blot analysis of lysates from A-431 cells using Phospho-VEGF Receptor 2-Y1175 Rabbit mAb (STJ11107732) at 1:3900 dilution incubated overnight at 4C. A-431 cells were treated by EGF (50 ng/ml) at 37C for 30 minutes after serum-starvation overnight. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 1s.

Anti-Phospho-VEGF Receptor 2-Y1175 antibody [SRM5543] (STJ11107732)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-Phospho-VEGFR-2-Tyr-801 antibody (STJA0041445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-VHL-Ser68 antibody (34-83 aa) (STJ91273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Vimentin-S39 Rabbit pAb (STJ117904) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37 °C for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S39 antibody (STJ117904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S56 pAb (STJ117902) at 1:3000 dilution or Vimentin antibody (STJ249342). HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S56 antibody (STJ117902)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Vimentin-S83 Rabbit pAb (STJ11101153) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37 °C for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S83 antibody (STJ11101153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S83 pAb (STJ117897) at 1:2000 dilution or Vimentin antibody (STJ26092). HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours or treated by Paclitaxel (100 nM/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S83 antibody (STJ117897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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Anti-Phospho-VIM-Ser39 antibody (STJ196472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of A549 cells using Phospho-Vimentin (S56) Polyclonal Antibody

Anti-Phospho-VIM-Ser56 antibody (31-80 aa) (STJ90927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of 293 cells using Phospho-Vimentin (S83) Polyclonal Antibody

Anti-Phospho-VIM-Ser83 antibody (56-105 aa) (STJ90926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HELA cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000

Anti-Phospho-VIM-Tyr38 antibody (STJ98572)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysate from Jurkat cells, using phospho-Vimentin (Phospho-Tyr61) antibody.

Anti-Phospho-VIM-Tyr61 antibody (STJ90435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-Vimentin-S34 antibody [YT33] (STJA0019156)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-Phospho-Vimentin-S56 antibody [4A4] (STJA0019155)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Anti-Phospho-Vimentin-S83 antibody [MO82] (STJA0019157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£195.50 - £1,319.50

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Phospho-Vimentin Ser39 antibody western blot HeLa STJA0035697

Anti-Phospho-Vimentin-Ser39 antibody [R38-1F-8] (STJA0035697)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Phospho-Vimentin Ser56 antibody immunocytochemistry HeLa STJA0034994

Anti-Phospho-Vimentin-Ser56 antibody [R01-0E2] (STJA0034994)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Phospho-Vimentin Ser72 antibody western blot rat brain STJA0034995

Anti-Phospho-Vimentin-Ser72 antibody [R04-1C8] (STJA0034995)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Rat

£133.50 - £281.50

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Phospho-Vimentin Ser72 antibody western blot HeLa Calyculin A STJA0035655

Anti-Phospho-Vimentin-Ser72 antibody [R37-4W-5] (STJA0035655)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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