Rabbit Monoclonal Antibodies | St John's Laboratory

Western blot analysis of lysates from Karpas 299 cells using Phospho-IRAK4-Ser346 Rabbit monoclonal antibody (STJ11105898) at 1:1000 dilution. Karpas 299 cells were treated by IL-1 Beta (200 ng/ml) at 37 °C for 15 minutes after serum starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 75s.

Anti-Phospho-IRAK4-Ser346 antibody [S5898RM] (STJ11105898)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Phospho-IRE1 Alpha-Ser724 antibody [PT1195R] (STJA0040223)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of lysates from HeLa cells, using Phospho-IRF3-S386 Rabbit mAb (STJ11102584) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-IRF3-S386 antibody [S4MR] (STJ11102584)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Phospho-IRF3-S396 antibody [SRM13691] (STJ11108421)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Phospho-IRF3-Ser386 antibody [PT0984R] (STJA0039769)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human

£285.00 - £540.00

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Anti-Phospho-IRF3-Ser396 antibody [PT0875R] (STJA0039777)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£285.00 - £540.00

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Anti-Phospho-IRS1-S612/S636-S612/S636 antibody [SRM15141] (STJ11109871)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Phospho-JAK1-Tyr1034/1035 antibody [PT0991R] (STJA0040088)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-JAK2-Y1007/1008-Y1007/1008 antibody [SRM15155] (STJ11109885)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£0.00

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Confocal imaging of paraffin-embedded Rat brain tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit monoclonal antibody (STJ11105826, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-Phospho-JNK1 + JNK2 + JNK3-T183/Y185-T183/Y185-T221/Y223 antibody [S5826RM] (STJ11105826)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Anti-Phospho-JNK1/2/3-Thr183/183/221 antibody [PT0914R] (STJA0039805)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-c-Jun-S63 Rabbit mAb (STJ11102553) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-JUN-S63 antibody [S3MR] (STJ11102553)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-c-Jun-T91 Rabbit mAb (STJ11102592) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-JUN-T91 antibody [S2MR] (STJ11102592)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-Phospho-Jun/JunD-Ser73/100 antibody [PT0912R] (STJA0039803)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of extracts of HeLa cells, using Phospho-JunD-S255 antibody (STJ11105821) at1:2000 dilution. Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-JUND-S255 antibody [S5821RM] (STJ11105821)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-JunD-S255 Rabbit mAb (STJ11102638) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-JUND-S255 antibody [S8MR] (STJ11102638)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£118.50 - £521.50

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Western blot analysis of A-431 lysates, using Phospho-VEGF Receptor 2-Y1175 antibody (STJ11105860) at 1:3000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-KDR-Y1175 antibody [S5860RM] (STJ11105860)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£143.50 - £618.50

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Anti-Phospho-LATS1-T1079+LATS2-T1041-T1079+T1041 antibody [SRM15143] (STJ11109873)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-LATS1-Thr1079 antibody [PT1358R] (STJA0040058)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£150.00 - £540.00

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Western blot analysis of 3T3-L1 cells, using Phospho-HSL-S660 Rabbit monoclonal antibody (STJ11105885) at 1:1000 dilution. 3T3-L1 cells that were not differentiated or differentiated into adipocytes treated with 0. 06mol/L alcohol and treated with isoproterenol (10 Mu M, 20 min). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-LIPE-S660 antibody [S5885RM] (STJ11105885)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£143.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-HSL-S853 Rabbit mAb (STJ11102644) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-LIPE-S853 antibody [S4MR] (STJ11102644)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£118.50 - £521.50

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Anti-Phospho-LKB1-Ser428 antibody [PT1347R] (STJA0040013)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-LRRK2-S935 Rabbit mAb (STJ11102647) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-LRRK2-S935 antibody [S7MR] (STJ11102647)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Lyn-Y396 Rabbit mAb (STJ11102620) at 1:1000 dilution. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-LYN-Y396 antibody [S0MR] (STJ11102620)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit mAb (STJ11102602) at 1:1000 dilution. Both HeLa cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by PMA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-MAP2K1-S298 antibody [S2MR] (STJ11102602)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Western blot analysis of lysates from C6 cells, using Phospho-MEK1-T292 Rabbit mAb (STJ11102603) at 1:1000 dilution. C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-MAP2K1-T292 antibody [S3MR] (STJ11102603)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-Phospho-MAP2K4-S257 antibody [SRM13701] (STJ11108431)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from 293T cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (STJ11102568) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (STJ11102154) at1:3000 dilution (lower) incubated overnight at 4 °C. 293T cells were treated by PMA (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 30 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 10s.

Anti-Phospho-MAPK1/MAPK3-T202/Y204 & T185/Y187 antibody [S8MR] (STJ11102568)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of C6, using Phospho-p38 MAPK-T180/Y182 Rabbit monoclonal antibody (STJ11105903) at 1:1000 dilution. C6 cells were treated by Anisomycin (25 ug/ml) at 37 °C for 20 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-MAPK14-T180/Y182 antibody [S5903RM] (STJ11105903)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (STJ113512) at 1:3000 dilution. HeLa cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-MAPK8/MAPK9/MAPK10-T183/T183/T221 antibody [S2MR] (STJ113512)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of lysates from Mouse brain, using Phospho-Tau-S396 Rabbit mAb (STJ11102609) at 1:1000 dilution. Mouse brain were treated by CIP (20 Mu L/400 Mu L) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-MAPT-S396 antibody [S9MR] (STJ11102609)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from SH-SY5Y cells using Phospho-Tau-S404 Rabbit monoclonal antibody (STJ11105855) at 1:5000 dilution. SH-SY5Y cells were treated by Okadaic Acid (100 nM) at 37 °C for 1 hour and Calyculin A (100nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 30 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-Phospho-MAPT-S404 antibody [S5855RM] (STJ11105855)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from 293T cells transfected with CDK5+CDK5R1+Tau and CDK5+CDK5R1+mutant Tau (T181/T217A) using Phospho-Tau-T181 Rabbit monoclonal antibody (STJ11105822) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-MAPT-T181 antibody [S5822RM] (STJ11105822)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Confocal imaging of paraffin-embedded Rat brain tissue using Phospho-Tau-T231 Rabbit mAb (STJ11102551, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-Phospho-MAPT-T231 antibody [S1MR] (STJ11102551)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of paraffin-embedded rat ovary using Phospho-MDM2-S166 Rabbit monoclonal antibody (STJ11105811) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-MDM2-S166 antibody [S5811RM] (STJ11105811)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-Phospho-MEK1/2-Ser217/221 antibody [PT0747R] (STJA0039208)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Anti-Phospho-Met-Tyr1234/1235 antibody [PT0745R] (STJA0039221)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Western blot analysis of various lysates, using Phospho-Met-Y1234/Y1235 antibody (STJ11105828) at1:2000 dilution. C2C12 and C6 cells were treated by HGF (40 ng/ml) at 37 °C for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-MET-Y1234/Y1235 antibody [S5828RM] (STJ11105828)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-Phospho-MKK3+MKK6-S189+S207 antibody [SRM13703] (STJ11108433)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Phospho-MKK3-S189+MKK6-S207-S189+S207 antibody [SRM15130] (STJ11109860)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Phospho-MKK3/MKK6-Ser189+Ser207 antibody [PT1372R] (STJA0040377)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-MLKL-Ser345 antibody [PT0987R] (STJA0039926)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Anti-Phospho-MSK1-Ser360 antibody [PT0174R] (STJA0039650)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-MST1-T183+MST2-T180-T183+T180 antibody [SRM15150] (STJ11109880)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from 293T cells, using Phospho-mTOR-S2448 Rabbit mAb (STJ11102554) at 1:1000 dilution. 293T cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight or treated by IGF-1 (50 ng/mL) at 37 °C for 5 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-mTOR-S2448 antibody [S4MR] (STJ11102554)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of various lysates using Phospho-mTOR-S2481 (STJ11102570) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by IGF-1 (50 ng/mL) at 37 °C for 5 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-MTOR-S2481 antibody [S0MR] (STJ11102570)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-Phospho-mTOR-Ser2448 antibody [PT0498R] (STJA0039463)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-Phospho-mTOR-Ser2481 antibody [PT0898R] (STJA0039791)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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