• Immunohistochemistry analysis of paraffin-embedded human colon using GTPBP4 Rabbit monoclonal antibody (STJ11102145) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using GTPBP4 Rabbit monoclonal antibody (STJ11102145) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GTPBP4 antibody (2-92) [S5MR] (STJ11102145)

SKU:
STJ11102145

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GTPBP4 (2-92) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GTPBP4
Gene ID: 23560
Uniprot ID: GTPB4_HUMAN
Immunogen Region: 2-92
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2-92 of human GTPBP4 (Q9BZE4).
Immunogen Sequence: AHYNFKKITVVPSAKDFIDL TLSKTQRKTPTVIHKHYQIH RIRHFYMRKVKFTQQNYHDR LSQILTDFPKLDDIHPFYAD LMNILYDKDHY
Function Involved in the biogenesis of the 60S ribosomal subunit. Acts as TP53 repressor, preventing TP53 stabilization and cell cycle arrest.
Protein Name Gtp-Binding Protein 4
Chronic Renal Failure Gene Protein
Gtp-Binding Protein Ngb
Nucleolar Gtp-Binding Protein 1
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Gtp-Binding Protein 4 antibody
Anti-Chronic Renal Failure Gene Protein antibody
Anti-Gtp-Binding Protein Ngb antibody
Anti-Nucleolar Gtp-Binding Protein 1 antibody
Anti-GTPBP4 antibody
Anti-CRFG antibody
Anti-NOG1 antibody

Information sourced from Uniprot.org

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