• Immunofluorescence analysis of NIH-3T3 cells using Guanylyl Cyclase beta 1 (GUCY1B3) (GUCY1B3) Rabbit monoclonal antibody (STJ11101926) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human brain using Guanylyl Cyclase beta 1 (GUCY1B3) (GUCY1B3) Rabbit monoclonal antibody (STJ11101926) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Guanylyl Cyclase beta 1 (GUCY1B3) (GUCY1B3) Rabbit monoclonal antibody (STJ11101926) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-GUCY1B3 antibody (1-100) [S6MR] (STJ11101926)

SKU:
STJ11101926

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Guanylyl Cyclase beta 1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GUCY1B1
Gene ID: 2983
Uniprot ID: GCYB1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Guanylyl Cyclase beta 1 (GUCY1B3) (Q02153).
Immunogen Sequence: MYGFVNHALELLVIRNYGPE VWEDIKKEAQLDEEGQFLVR IIYDDSKTYDLVAAASKVLN LNAGEILQMFGKMFFVFCQE SGYDTILRVLGSNVREFLQN
Tissue Specificity Detected in brain cortex and cerebellum (at protein level).
Function Mediates responses to nitric oxide (NO) by catalyzing the biosynthesis of the signaling molecule cGMP.
Protein Name Guanylate Cyclase Soluble Subunit Beta-1
Gcs-Beta-1
Guanylate Cyclase Soluble Subunit Beta-3
Gcs-Beta-3
Soluble Guanylate Cyclase Small Subunit
Database Links Reactome: R-HSA-392154
Reactome: R-HSA-445355
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Guanylate Cyclase Soluble Subunit Beta-1 antibody
Anti-Gcs-Beta-1 antibody
Anti-Guanylate Cyclase Soluble Subunit Beta-3 antibody
Anti-Gcs-Beta-3 antibody
Anti-Soluble Guanylate Cyclase Small Subunit antibody
Anti-GUCY1B1 antibody
Anti-GUC1B3 antibody
Anti-GUCSB3 antibody
Anti-GUCY1B3 antibody

Information sourced from Uniprot.org

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