Western Blot Antibodies | St John's Laboratory

Western blot analysis of extracts of Rat liver, using BHMT rabbit polyclonal antibody antibody (STJ22796) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BHMT antibody (177-406) (STJ22796)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using BHMT Rabbit monoclonal antibody (STJ11102269) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180S.

Anti-BHMT antibody (300-406) [S9MR] (STJ11102269)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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STJ71251 (0. 03µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-BHMT antibody (C-Term) (STJ71251)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Western blot analysis using BHMT monoclonal antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY BHMT cDNA (2).

Anti-BHMT antibody [8C11H5] (STJ97863)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-BHMT2 antibody (STJA0004764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£140.50 - £415.50

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Anti-BHMT2 antibody (STJA0004765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£140.50 - £415.50

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Anti-BHMT2 antibody (STJA0008671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-BICD2 antibody (400-450 aa) (STJ13100763)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Western blot analysis of extracts of various cell lines, using BICD2 antibody (STJ112330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BICD2 antibody (440-620) (STJ112330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-BICD2 antibody (780-820 aa) (STJ13100764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-BICDL2 antibody (STJA0009516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BICRAL antibody (17-67 aa) (STJ194351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of NIH-3T3 cells using Bid Rabbit polyclonal antibody (STJ22797) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Bid antibody (1-195) (STJ22797)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and Bid Rabbit monoclonal antibody knockdown (KD) 293T (KD) cells, using Bid Rabbit monoclonal antibody (STJ11105057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BID antibody (1-195) [S5057RM] (STJ11105057)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of various cells using BID Polyclonal Antibody diluted at 1：1000

Anti-BID antibody (44-93 aa) (STJ91853)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ70684 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-BID antibody (C-Term) (STJ70684)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human

£312.50

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Western blot analysis using BID monoclonal antibody against HeLa (1) , A431 (2) , Jurkat (3) , A549 (4) , HepG2 (5) , and HEK293 (6) cell lysate.

Anti-BID antibody [3C5] (STJ97864)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-BID antibody [PT0386R] (STJA0039148)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human

£0.00

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Anti-Bif-1 antibody (150-200) (STJ13100127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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STJ70058 staining (0. 1µg/ml) of HEK293 (A) and A431 (B) cell lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.

Anti-BIF-1/SH3GLB1 antibody (C-Term) (STJ70058)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Western blot analysis of lysates from Raji cells, using BIK Rabbit pAb (STJ11107023). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-BIK antibody (1-130 aa) (STJ11107023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using NBK Polyclonal Antibody diluted at 1：500

Anti-BIK antibody (18-67) (STJ94348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ70563 staining (0. 5µg/ml) of K562 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-BIM antibody (C-Term) (STJ70563)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Anti-Bim antibody [PT0603R] (STJA0039403)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunofluorescence analysis of U2OS cells using BIN1 antibody (STJ22799). Blue: DAPI for nuclear staining.

Anti-BIN1 antibody (230-409) (STJ22799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Anti-BIN1 antibody (40-90 aa) (STJ13100758)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Western blot analysis of mouse brain cells using Amphiphysin II Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-BIN1 antibody (C-Term) (STJ96681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Mouse/Rat

£48.00 - £252.00

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STJ71638 (0. 1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Anti-BIN1 antibody (Internal) (STJ71638)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Anti-BIN1/Amphiphysin-II antibody [N481/113] (STJA0020601)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£341.50 - £1,447.50

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Anti-BIN1/Amphiphysin-II antibody [N481/33] (STJA0020603)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£341.50 - £1,447.50

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Anti-BIN1/Amphiphysin-II antibody [N481/3] (STJA0020602)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£341.50 - £1,447.50

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Anti-BIN2 antibody (260-340 aa) (STJ191822)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Rosette leaves, using BIN2 Rabbit polyclonal antibody (STJ11104219) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-BIN2 antibody (281-380) (STJ11104219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

A.thaliana

£146.50 - £642.50

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Western blot analysis of various lysates using BIN3 Rabbit polyclonal antibody (STJ117673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BIN3 antibody (1-253) (STJ117673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from 3T3 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BIN3 antibody (184-234 aa) (STJ195772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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STJ71405 (0. 3µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Bin3 antibody (Internal) (STJ71405)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of recombinant Biotin-Goat-Mouse IgG Protein using Biotin Rabbit polyclonal antibody (STJ11101533) at 1:1000 dilution. NA NA Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. NA NA Lysates/proteins: 100 ng per lane. NA NA Blocking buffer: 3% nonfat dry milk in TBST. NA NA Detection: ECL Basic Kit. NA NA Exposure time: 45s.

Anti-Biotin antibody (STJ11101533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Species independent

£112.50 - £524.50

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Anti-Biotin antibody [Hyb-8] (STJ16100225)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB/IF/IHC

£287.50 - £1,324.50

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Anti-Biotin antibody [M33] (STJA0019295)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/SPR/WB

Reactivity :

General

£174.50 - £250.50

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Anti-Biotin antibody [M33] (STJA0021648)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

General

£199.50 - £283.50

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Western blot analysis of various lysates, using Biotin Rabbit monoclonal antibody (STJ11103412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Biotin antibody [S2MR] (STJ11103412)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Other

£146.50 - £642.50

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Western blot analysis of various lysates, using Biotin Rabbit monoclonal antibody (STJ11104796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Biotin antibody [S4796RM] (STJ11104796)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Other

£146.50 - £642.50

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Western blot analysis of various lysates using Biotin Rabbit monoclonal antibody (STJ11104797) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Biotin antibody [S4797RM] (STJ11104797)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Other

£146.50 - £642.50

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Anti-Biotin antibody [SAA0349] (STJA0019296)

Clonality :

Monoclonal

Host :

Mouse

Applications :

SPR/WB

Reactivity :

General

£174.50 - £250.50

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STJ72947 (0. 5µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-biotinidase antibody (372-385) (STJ72947)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of extracts of various cell lines, using cIAP1 antibody (STJ11101767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-BIRC2 antibody (1-100) [S7MR] (STJ11101767)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Western blot analysis of lysates from Jurkat cells, using cIAP1 Rabbit pAb (STJ22800) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-BIRC2 antibody (1-300) (STJ22800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of BALA-3T3 cells using cIAP1 rabbit polyclonal antibody (STJ114903) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-BIRC2 antibody (331-618) (STJ114903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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