• STJ70537 (2µg/ml) staining of Rat Skeletal Muscle lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70537 (0. 5µg/ml) staining of NIH3T3 nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70537 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70537 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70537 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-BAF53A and BAF53B antibody (C-Term) (STJ70537)

SKU:
STJ70537-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-BAF53A and BAF53B (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the nuclei of A431 and U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ACTL6B
Gene ID: 51412
Uniprot ID: ACL6B_HUMAN
Immunogen Region: C-Term
Accession Number: NP_004292.1; NP_817126.1; NP_057272.1
Specificity: This antibody is expected to recognise BAF53A isoforms 1 and 2 and BAF53B, which are almost identical
Immunogen Sequence: YEEGGKQCVERKCP
Protein Name Actin-Like Protein 6b
53 Kda Brg1-Associated Factor B
Actin-Related Protein Baf53b
Arpnalpha
Brg1-Associated Factor 53b
Baf53b
Database Links Reactome: R-HSA-3214858
Reactome: R-HSA-8939243
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Actin-Like Protein 6b antibody
Anti-53 Kda Brg1-Associated Factor B antibody
Anti-Actin-Related Protein Baf53b antibody
Anti-Arpnalpha antibody
Anti-Brg1-Associated Factor 53b antibody
Anti-Baf53b antibody
Anti-ACTL6B antibody
Anti-ACTL6 antibody
Anti-BAF53B antibody

Information sourced from Uniprot.org

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