• Immunoprecipitation analysis of 300 Mu g extracts of K-562 cells using 3 Mu g BACH1 antibody (STJ11100910). Western blot was performed from the immunoprecipitate using BACH1 antibody (STJ11100910) at a dilution of 1:600.
  • Western blot analysis of lysates from HEL cells, using BACH1 Rabbit polyclonal antibody (STJ11100910) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-BACH1 antibody (1-736) (STJ11100910)

SKU:
STJ11100910

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Host: Rabbit
Applications: WB/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BACH1 (1-736) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BACH1
Gene ID: 571
Uniprot ID: BACH1_HUMAN
Immunogen Region: 1-736
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-736 of human BACH1 (NP_996749.1).
Immunogen Sequence: MSLSENSVFAYESSVHSTNV LLSLNDQRKKDVLCDVTIFV EGQRFRAHRSVLAACSSYFH SRIVGQADGELNITLPEEVT VKGFEPLIQFAYTAKLILSK ENVDEVCKCVEFLSVHNIEE SCFQFLKFKFLDSTADQQEC PRKKCFSSHCQKTDLKLSLL DQRDLETDEVEEFLENKNVQ TPQCKLRRYQGNAKASPPLQ DSASQTYESMCLEKDAALAL PSLCPKYRKFQKAFGTDRV
Post Translational Modifications Ubiquitinated by the SCF(FBXL17) complex, leading to its degradation by the proteasome.
Function Transcriptional regulator that acts as repressor or activator, depending on the context. Binds to NF-E2 DNA binding sites. Plays important roles in coordinating transcription activation and repression by MAFK. Together with MAF, represses the transcription of genes under the control of the NFE2L2 oxidative stress pathway.
Protein Name Transcription Regulator Protein Bach1
Btb And Cnc Homolog 1
Ha2303
Database Links Reactome: R-HSA-9707587
Reactome: R-HSA-9707616
Reactome: R-HSA-9708530
Reactome: R-HSA-9818027
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Regulator Protein Bach1 antibody
Anti-Btb And Cnc Homolog 1 antibody
Anti-Ha2303 antibody
Anti-BACH1 antibody

Information sourced from Uniprot.org

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