Anti-BAG1 antibody [2C12-A8-1D7] (STJ99243)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant human Bag1 protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-BAG family molecular chaperone regulator 1 for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 2C12-A8-1D7 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BAG1 |
| Gene ID | 573 |
| Uniprot ID | BAG1_HUMAN |
| Immunogen | Purified recombinant human Bag1 protein fragments expressed in E.coli. |
| Specificity | Transfected |
Additional Info
| Post Translational Modifications | Ubiquitinated.mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. |
| Function | Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. Involved in the STUB1-mediated proteasomal degradation of ESR1 in response to age-related circulating estradiol (17-beta-estradiol/E2) decline, thereby promotes neuronal apoptosis in response to ischemic reperfusion injury. |
| Protein Name | Bag Family Molecular Chaperone Regulator 1Bag-1Bcl-2-Associated Athanogene 1 |
| Database Links | Reactome: R-HSA-3371453 |
| Cellular Localisation | Isoform 1: NucleusCytoplasmIsoform 1 Localizes Predominantly To The NucleusIsoform 2: CytoplasmNucleusIsoform 2 Localizes To The Cytoplasm And Shuttles Into The Nucleus In Response To Heat ShockIsoform 4: CytoplasmIsoform 4 Localizes Predominantly To The CytoplasmThe Cellular Background In Which It Is Expressed Can Influence Whether It Resides Primarily In The Cytoplasm Or Is Also Found In The NucleusIn The Presence Of Bcl2Localizes To Intracellular Membranes (What Appears To Be The Nuclear Envelope And Perinuclear Membranes) As Well As Punctate Cytosolic Structures Suggestive Of Mitochondria |
| Alternative Antibody Names | Anti-Bag Family Molecular Chaperone Regulator 1 antibodyAnti-Bag-1 antibodyAnti-Bcl-2-Associated Athanogene 1 antibodyAnti-BAG1 antibodyAnti-HAP antibody |
Information sourced from Uniprot.org