Western Blot Antibodies | St John's Laboratory

Western blot analysis of various cells using AR-Beta 2 Polyclonal Antibody

Anti-ADRB2 antibody (321-370 aa) (STJ91726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ70778 (0. 5µg/ml) staining of HepG2 cell ysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ADRB2R/ADRB2 antibody (C-Term) (STJ70778)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Dog/Cow

£312.50

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Western blot analysis of various lysates using ADRB3 Rabbit pAb (STJ111338) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-ADRB3 antibody (1-100) (STJ111338)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of K562 cells using AR-Beta 3 Polyclonal Antibody diluted at 1：1000

Anti-ADRB3 antibody (250-299 aa) (STJ91728)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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STJ71297 (0. 03µg/ml) staining of Human Brain (Cerebral Cortex) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ADRB3 antibody (N-Term) (STJ71297)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of extracts of various cell lines, using ADRM1 antibody (STJ22536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ADRM1 antibody (108-407) (STJ22536)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ADRM1 antibody (STJA0007048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of mouse testis, using ADSL rabbit polyclonal antibody (STJ28200) at 1:700 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-ADSL antibody (1-310) (STJ28200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ADSL antibody (1-484 aa) [SRM5256] (STJ11107158)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-ADSL antibody (STJA0007586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using ADSS antibody (STJ28599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ADSS antibody (1-280) (STJ28599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ADSS1 antibody (14-64 aa) (STJ194983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using AdSS 2 antibody (STJ11104127) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ADSS2 antibody (1-180) [S4127RM] (STJ11104127)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-ADSS2 antibody (STJA0007461)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-AE3/SLC4A3 antibody [N242/12] (STJA0020871)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

£341.50 - £1,447.50

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Anti-AEBP1 antibody (780-810 aa) (STJ191759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using AEBP1 Rabbit polyclonal antibody (STJ118768) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AEBP1 antibody (999-1158) (STJ118768)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AEBP2 antibody (155-205 aa) (STJ194417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse brain using AEBP2 antibody (STJ29537) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-AEBP2 antibody (234-503) (STJ29537)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using AES Rabbit pAb (STJ110184) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-AES antibody (1-264) (STJ110184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and AES knockout (KO) HeLa cells, using [KO Validated] AES Rabbit polyclonal antibody (STJ11100820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AES antibody (1-264) (STJ11100820)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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STJ72590 (0. 3µg/ml) staining of Human Cerebral Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AES antibody (N-Term) (STJ72590)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat

£312.50

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STJ71985 (0. 3µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Afamin/alpha Albumin antibody (Internal) (STJ71985)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Western blot analysis of extracts of various cell lines, using AFAP1 antibody (STJ22538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AFAP1 antibody (461-730) (STJ22538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-AFAP1 antibody (STJA0007195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AFAP1L1 antibody (180-230 aa) (STJ193785)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-AFDN antibody (STJA0009307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-AFDN-DT antibody (11-61 aa) (STJ197516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AF4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-AFF1 antibody (1-50 aa) (STJ91505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-AFF2 antibody (1104-1154 aa) (STJ197643)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using AFF4 antibody (STJ22540) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Basic Kit. _Exposure time: 30s.

Anti-AFF4 antibody (1-220) (STJ22540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-AFF4 antibody (30-110 aa) (STJ190525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-AFG2A antibody (9-59) (STJA0006456)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-AFG2B antibody (STJA0009038)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of various lysates, using AFG3L2 Rabbit pAb (STJ11106949) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-AFG3L2 antibody (538-797 aa) (STJ11106949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-AFG3L2 antibody (538-797 aa) [SRM5332] (STJ11107238)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g AFG3L2 antibody (STJ117588). Western blot was performed from the immunoprecipitate using AFG3L2 antibody (STJ117588) at a dilution of 1:1000.

Anti-AFG3L2 antibody (538-797) (STJ117588)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 1, mouse-heart 2, 293T cells using primary antibody diluted at 1:500 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-AFG3L2 antibody (744-793 aa) (STJ99339)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Aflatoxin B1 & B2 antibody [AFT14/CBL03] (STJ16100224)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Aspergillus

£377.50

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Western blot analysis of lysates from Rat heart, using AFM Rabbit polyclonal antibody (STJ117448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.

Anti-AFM antibody (260-599) (STJ117448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Anti-AFMID antibody (STJA0007499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Alpha-Fetoprotein (AFP) antibody (STJ11104685) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AFP antibody (19-609) [S4685RM] (STJ11104685)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of extracts of HeLa cells, using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP) ) mouse monoclonal antibody (STJ119899) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AFP antibody (19-609) [S9MM] (STJ119899)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-AFP (PT0679) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: HepG2 Lane 3: mouse liverPredicted band size: 69kDaObserved band size: 69kDa

Anti-AFP antibody (350-450 aa) (STJ196165)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of various lysates, using Alpha-Fetoprotein (AFP) Rabbit polyclonal antibody (STJ22541) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AFP antibody (360-609) (STJ22541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of mouse liver, mouse serum cells using AFP Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-AFP antibody (371-420 aa) (STJ96505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of HepG2 cells (upper left) and LO2 cells (negative sample control) (upper right) using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP) ) Rabbit polyclonal antibody (red, A17457) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AFP antibody (450-550) (STJ113691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot with Anti-AFP Antibody (STJ500051) and peptides and human serum albumin at 1:500 dilution in buffer.

Anti-AFP antibody (Full Length) (STJ500051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£427.50

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