Immunofluorescence Antibodies | St John's Laboratory

Anti-ALIX antibody [SRM14813] (STJ11109543)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-beta Catenin antibody [R07-9D5] (STJA0034078)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of Beta-catenin. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-beta Catenin antibody [SM4939] (STJA0004939)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-beta Catenin antibody [SM5169] (STJA0005169)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£153.50 - £228.50

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Immunofluorescence analysis of U2OS cells using Beta-Catenin antibody (STJ113475) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CTNNB1 antibody (1-100) (STJ113475)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £618.50

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Western blot analysis of extracts of HeLa cells, using Beta-Catenin antibody (STJ11101750) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CTNNB1 antibody (1-100) [S0MR] (STJ11101750)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of various cells using Catenin-Beta Polyclonal Antibody diluted at 1：1000

Anti-CTNNB1 antibody (3-52 aa) (STJ92041)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/CoIP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Catenin-Beta 1 Polyclonal Antibody diluted at 1：1000

Anti-CTNNB1 antibody (626-675 aa) (STJ92042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Beta-catenin antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 94 kDa

Anti-CTNNB1 antibody (700-781 aa) [ABT-Catenin] (STJ197008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Immunohistochemistry of mouse lung using anti-CTNNB1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTNNB1 antibody at 1:500:DAB detection.

Anti-CTNNB1 antibody (731aa C-Term) (STJ140021)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF/IHC-P/IHC-F

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

£94.50 - £440.50

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Western blot analysis using Catenin-Beta monoclonal antibody against CTNNB1-hIgGFc transfected HEK293 cell lysate.

Anti-CTNNB1 antibody [4D5] (STJ97896)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, Catenin-Beta monoclonal antibody (4F2) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-CTNNB1 antibody [4F2] (STJ97420)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat/Zebrafish

£49.00 - £261.00

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Anti-CTNNB1 nanobody [SAA1197] (STJN000205)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/IF/IHC/IP/SPR/WB

Reactivity :

Human

£209.50 - £1,333.50

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STJ73125 (0. 3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CTNNB1/catenin beta-1 antibody (108-121) (STJ73125)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ73756 (0. 1µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CTNNB1/catenin beta-1 antibody (Internal) (STJ73756)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Immunofluorescence analysis of L929 cells using CYTH1 Rabbit polyclonal antibody (STJ117546) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CYTH1 antibody (1-70) (STJ117546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Human placental mesenchymal stromal cells modulate IFN-γ and IL-10 secretion by CD4+T cells via CD73, and alleviate intestinal damage in mice with graft-versus-host disease. INTERNATIONAL IMMUNOPHARMACOLOGY Xiying Luan WB Human, mouse intestine, colon NCM460 cell, Caco-2 cell

Anti-OCLN antibody (350-400 aa) (STJ192969)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of Mouse lung, using Occludin Rabbit polyclonal antibody (STJ111161) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-OCLN antibody (400-522) (STJ111161)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-OCLN antibody (AA range: 1-522) [PTR1394] (STJA0038890)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-OCLN antibody [PT0483R] (STJA0039506)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunofluorescence analysis of U-251MG cells using ALIX/ PDCD6IP antibody (STJ24927) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PDCD6IP antibody (1-180) (STJ24927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from C6 cells, using Phospho-Beta-Catenin-S675 Rabbit pAb (STJ117893) at 1:1000 dilution. C6 cell lysates were treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-CTNNB1-S675 antibody (STJ117893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Catenin-beta (Phospho-Ser33) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CTNNB1-Ser33 antibody (17-66 aa) (STJ90206)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of human-liver-cancer tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-CTNNB1-Ser37 antibody (3-52 aa) (STJ90207)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from COS7 cells treated with UV 15', using Catenin-beta (Phospho-Tyr489) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CTNNB1-Tyr489 antibody (455-504 aa) (STJ90928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of 293 cells using Phospho-Catenin-Beta (Y654) Polyclonal Antibody

Anti-Phospho-CTNNB1-Tyr654 antibody (620-669 aa) (STJ90209)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HeLa, 293 cells using CD148 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PTPRJ antibody (861-910 aa) (STJ96765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Recombinant-beta Catenin antibody [SM0544] (STJA0010544)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-Recombinant-beta Catenin antibody [SR0538] (STJA0010538)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-Recombinant-beta Catenin antibody [SR0543] (STJA0010543)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-Recombinant-CTNNB1 antibody [RM1U86] (STJA0024466)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-ZO1 tight junction protein antibody [SR0620] (STJA0010620)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-ZO1 antibody at 1:2500 dilution; 50 µg of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-TJP1 antibody (1580aa C-Term) (STJ140055)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF/IHC-P/IHC-F

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian/Other

£94.50 - £440.50

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Anti-TJP1 antibody (1600-1700 aa) (STJ191514)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Pig

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using ZO-1 antibody (STJ113790) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TJP1 antibody (18-1748) (STJ113790)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of MCF7 cells using ZO-1 Rabbit polyclonal antibody (STJ25857) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TJP1 antibody (18-1748) (STJ25857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of A549 cells, using Ubinuclein Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-UBN1 antibody (161-210 aa) (STJ96171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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STJ71854 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showingcellular junction staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-ZO-1 antibody (Internal) (STJ71854)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human

£312.50

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Anti-ZO1 antibody [PT0639R] (STJA0039325)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-ZO1 tight junction protein antibody [SM0454] (STJA0010454)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£153.50 - £228.50

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