• STJ72883 (2µg/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ72883 Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
  • STJ72883 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72883 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-5HT2A Receptor antibody (296-309) (STJ72883)

SKU:
STJ72883-100

£303.50
Shipping:
Free Shipping
Current Stock:
Host: Goat
Applications: Pep-ELISA/IHC/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-5HT2A Receptor (296-309) is suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-2µg/ml
IF-Strong expression of the protein seen in the cytoplasm of A549 cells. 10µg/ml
FC-Flow cytometric analysis of A549 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: HTR2A
Gene ID: 3356
Uniprot ID: 5HT2A_HUMAN
Immunogen Region: 296-309
Accession Number: NP_000612.1; NP_001159419.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_000612.1; NP_001159419.1).
Immunogen Sequence: QRSIHREPGSYTGR
Function G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca(2+) ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction. (Microbial infection) Acts as a receptor for human JC polyomavirus/JCPyV.
Protein Name 5-Hydroxytryptamine Receptor 2a
5-Ht-2
5-Ht-2a
Serotonin Receptor 2a
Database Links Reactome: R-HSA-390666
Reactome: R-HSA-416476
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Dendrite
Axon
Cytoplasmic Vesicle
Membrane
Caveola
Presynapse
Alternative Antibody Names Anti-5-Hydroxytryptamine Receptor 2a antibody
Anti-5-Ht-2 antibody
Anti-5-Ht-2a antibody
Anti-Serotonin Receptor 2a antibody
Anti-HTR2A antibody
Anti-HTR2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance