Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of various lysates using CD47 Rabbit pAb (STJ23004) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-CD47 antibody (292-304) (STJ23004)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 3T3 cells using CD47 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-CD47 antibody (61-110 aa) (STJ96874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-CD47 antibody (78-89 aa) (STJ73858)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human

£312.50

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STJ73432 (0. 3µg/ml) staining of Human Hippocampus (A) and HeLa (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD47 antibody (Internal) (STJ73432)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/IHC

Reactivity :

Human/Dog

£312.50

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Anti-CD47 antibody [PT1437R] (STJA0040342)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-JNK1 antibody [SRM14610] (STJ11109340)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of various cells using JNK1/2/3 Polyclonal Antibody diluted at 1：1000

Anti-JNK1/2/3 antibody (166-215 aa) (STJ93805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Chicken/Fish/Pig

£49.00 - £261.00

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Anti-LOXL2 antibody [SAA2215] (STJA0022478)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF

Reactivity :

Human

£209.50 - £1,333.50

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Immunofluorescence analysis of HeLa cells using JNK1 antibody (STJ24490) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAPK8 antibody (245-345) (STJ24490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot detection of JNK1 in C6 and 3T3 cell lysates using JNK1 mouse mAb (1:1000 diluted).Predicted band size:46, 54KDa.Observed band size:46KDa.

Anti-MAPK8 antibody [1A4-C5-F11] (STJ99225)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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Western blot analysis of various cells using JNK2 Polyclonal Antibody

Anti-MAPK9 antibody (246-295 aa) (STJ93807)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using JNK2 Rabbit pAb diluted at 1:200

Anti-MAPK9 antibody (STJ197370)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using Nanog antibody (STJ11104896) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-NANOG antibody (153-305) [S4896RM] (STJ11104896)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis using Nanog monoclonal antibody against NTERA-2 cell lysate (2).

Anti-NANOG antibody (20-166 aa) [1E6C4] (STJ98266)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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STJ70746 (0. 03µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-NANOG antibody (Internal) (STJ70746)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/IHC

Reactivity :

Human/Dog/Pig

£312.50

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Anti-Nanog antibody [PT0179R] (STJA0039699)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£150.00 - £540.00

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Immunofluorescence analysis of mouse liver cells using NR5A2 Rabbit polyclonal antibody (STJ115643) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NR5A2 antibody (300-541) (STJ115643)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using NR5A2 antibody (STJ28333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NR5A2 antibody (300-541) (STJ28333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of rat-testis tissue. 1, JNK1/2/3 (phospho Thr183) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-JNK1/2/3-Thr183 antibody (151-200 aa) (STJ90316)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

£49.00 - £261.00

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Immunofluorescence analysis of rat-liver tissue. 1, JNK1/2/3 (phospho Thr183/Y185) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-JNK1/2/3-Thr183/Y185 antibody (151-200 aa) (STJ90317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Chicken/Pig/Fish

£49.00 - £261.00

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Immunofluorescence analysis of human-lung tissue. 1, JNK1/2/3 (phospho Tyr185) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-JNK1/2/3-Tyr185 antibody (151-200 aa) (STJ91003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Oct-3/4 Polyclonal Antibody diluted at 1：1000

Anti-POU5F1 antibody (191-240) (STJ94598)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using Oct-3/4 monoclonal antibody against F9 cell lysate.

Anti-POU5F1 antibody (STJ98523)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC/IF

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using Oct-3/4 monoclonal antibody against NTERA-2 (1) cell lysate.

Anti-POU5F1 antibody [7E7] (STJ98287)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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STJ72514 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-PRDM14 antibody (414-427) (STJ72514)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Anti-Recombinant-CD47/MER6 antibody [RM1B02] (STJA0026772)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-JNK1/2/3 antibody [RM1C15] (STJA0026884)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IP/WB

Reactivity :

Human/Mouse/Rat/Cow/Dog/Monkey/Zebrafish

£300.50 - £457.50

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Anti-Recombinant-LOXL2 antibody [RM2L56] (STJA0026020)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-MAPK8 antibody [RM1W70] (STJA0024648)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IP/WB

Reactivity :

Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Phospho-JNK1/2/3-T183 + T183 + T221 antibody [RM1C42] (STJA0026911)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Human seminoma tissue was stained with Anti-SALL4 (ABT-SALL4) Antibody

Anti-SALL4 antibody (700-800 aa) [ABT-SALL4] (STJ197004)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human

£180.00 - £261.00

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Anti-SALL4 antibody (750-850) [PT2242] (STJA0039096)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF/ELISA

Reactivity :

Human

£49.00 - £261.00

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Immunofluorescence analysis of paraffin-embedded rat testis using Sall4 Rabbit polyclonal antibody (STJ118633) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SALL4 antibody (853-1053) (STJ118633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Sall4 antibody [PT0614R] (STJA0039383)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human

£150.00 - £540.00

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STJ71828 (1µg/ml) staining of Mouse Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Thrombospondin antibody (N-Term) (STJ71828)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-ZNF462 antibody (STJ191648)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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