Immunofluorescence Antibodies | St John's Laboratory

Immunofluorescence analysis of U-2 OS cells using Cyclin H Rabbit monoclonal antibody (STJ11101991) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CCNH antibody (224-323) [S1MR] (STJ11101991)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Western blot analysis of lysates from wild type (WT) and CDK7 knockout (KO) 293T cells, using [KO Validated] CDK7 Rabbit polyclonal antibody (STJ114808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDK7 antibody (211-346) (STJ114808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of various cells using Cdk7 Polyclonal Antibody

Anti-CDK7 antibody (291-340 aa) (STJ92203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded rat liver using CDK9 Rabbit polyclonal antibody (STJ114907) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDK9 antibody (153-372) (STJ114907)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g CDK9 rabbit monoclonal antibody (STJ11101280) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of CDK9 in representative gene loci (RAC1) , as shown in figure.

Anti-CDK9 antibody (273-372) [S0MR] (STJ11101280)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis using Cdk9 monoclonal antibody against HEK293 (1) and CDK9-hIgGFc transfected HEK293 (2) cell lysate.

Anti-CDK9 antibody [1B5A7] (STJ97949)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

$205.62 - $496.80

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Anti-Cdk9 antibody [PT1054R] (STJA0039967)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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CDK9 antibody western blot K562 C6 3T3 HeLa STJA0034160

Anti-CDK9 antibody [R03-5B9] (STJA0034160)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g CPSF3L antibody (STJ28649). Western blot was performed from the immunoprecipitate using CPSF3L antibody (STJ28649) at a dilution of 1:500.

Anti-CPSF3L antibody (331-600) (STJ28649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-CREPT antibody [3E10#] (STJA0019707)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IHC/WB

Reactivity :

Human

$269.79 - $1,820.91

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Western blot analysis of various cells using CTDSP1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CTDSP1 antibody (1-50 aa) (STJ92515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-CTDSPL antibody (Internal) (STJ71564)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human/Dog

$431.25

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Western blot analysis of various cells using CUL-3 Polyclonal Antibody diluted at 1：1000

Anti-CUL3 antibody (1-50 aa) (STJ92524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of HeLa cells using Cullin 3 antibody (STJ23291). Blue: DAPI for nuclear staining.

Anti-CUL3 antibody (468-768) (STJ23291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using Cullin 3 Rabbit polyclonal antibody (STJ118883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CUL3 antibody (569-768) (STJ118883)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Cyclin H antibody immunocytochemistry HeLa cells STJA0035172

Anti-Cyclin H antibody [R02-4I4] (STJA0035172)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

$184.23 - $388.47

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Western blot analysis of various cells using Dyrk1A Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-DYRK1A antibody (21-70 aa) (STJ92800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-HRPT2/Parafibromin antibody [SM5006] (STJA0005006)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$315.33

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Immunofluorescence analysis of A549 cells using INTS10 antibody (STJ115380). Blue: DAPI for nuclear staining.

Anti-INTS10 antibody (451-710) (STJ115380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of various lysates using LEO1 Rabbit pAb (STJ11103634) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-LEO1 antibody (340-570) (STJ11103634)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various cells using Mat1 Polyclonal Antibody

Anti-MNAT1 antibody (91-140 aa) (STJ94027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-NEDD4 antibody [PT1292R] (STJA0040043)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDK7 (Phospho-Thr170) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CDK7-Thr170 antibody (136-185 aa) (STJ90891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Chromatin immunoprecipitations were performed with cross-linked chromatin from 293F cells and Phospho-POLR2A-S2 (STJ11102585). The ChIP sequencing results indicate the enrichment pattern of Phospho-POLR2A-S2 in selected genomic region and representative gene loci (GAPDH) , as shown in figure.

Anti-Phospho-POLR2A-S2 antibody [S5MR] (STJ11102585)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq/CUT&Tag

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit mAb (STJ11102586) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours or treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by CIP (20uL/400ul) at 37 °C for 1 hour. C2C12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-POLR2A-S5 antibody [S6MR] (STJ11102586)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of VEC using p-Rpb1 (S1619) antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-POLR2A-Ser1619 antibody (1585-1634 aa) (STJ91031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Phospho-PP2A alpha antibody western blot rat kidney A431 STJA0035755

Anti-Phospho-PP2A alpha-Tyr307 antibody [R07-9C6] (STJA0035755)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of PP1-alpha (Phospho-Thr320) Antibody. The lane on the right is blocked with the PP1-alpha (Phospho-Thr320) peptide.

Anti-Phospho-PPP1CA-Thr320 antibody (281-330 aa) (STJ91134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of Hela cell. 1, PP2A-C Alpha (phospho Tyr307) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HSP70 monoclonal antibody (3G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-PPP2CA-Tyr307 antibody (260-309 aa) (STJ91049)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Phospho-POLR2A antibody western blot MCF-7 STJA0036402

Anti-Phospho-RNA Polymerase II Subunit B1-Ser2 antibody [R06-5Y1] (STJA0036402)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Phospho-POLR2A antibody western blot HeLa lysates STJA0036403

Anti-Phospho-RNA Polymerase II Subunit B1-Ser5 antibody [R08-4O2] (STJA0036403)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-Phospho-Rpb1-Ser2 antibody [PT1003R] (STJA0039928)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$393.30 - $745.20

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Anti-Phospho-Rpb1-Ser5 antibody [PT0923R] (STJA0039821)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Western blot analysis of various cells using Phospho-WNK1 (T60) Polyclonal Antibody

Anti-Phospho-WNK1-Thr60 antibody (24-73 aa) (STJ90776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug POLR2A Rabbit polyclonal antibody (STJ25045 1:100). Western blot was performed from the immunoprecipitate using POLR2A Rabbit polyclonal antibody (STJ25045) at a dilition of 1:1000.

Anti-POLR2A antibody (1-1970) (STJ25045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using POLR2A antibody (STJ113726) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-POLR2A antibody (1-260) (STJ113726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various cells using Rpb1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-POLR2A antibody (1585-1634 aa) (STJ95538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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RNA Polymerase II Subunit B1 antibody immunocytochemistry CEM STJA0012190

Anti-POLR2A antibody (1602-1610 aa) [R06-8G7] (STJA0012190)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

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Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells using 1 Mu g POLR2D antibody (STJ25047). Western blot was performed from the immunoprecipitate using POLR2D antibody (STJ25047) at a dilution of 1:1000.

Anti-POLR2D antibody (1-142) (STJ25047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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ICC IF of PPP1CA in HeLa cells with STJA0041532 antibody Alexa Fluor 488 green staining DAPI blue nuclear counterstain

Anti-PP1 Catalytic Subunit/PPP1CA antibody (30-299aa) [4G3] (STJA0041532)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

$302.91 - $458.85

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PPP1A antibody western blot A431 Jurkat HeLa STJA0032355

Anti-PP1C alpha antibody [1C11-C10-H5-E9] (STJA0032355)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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PPP1A antibody western blot K562 3T3 HeLa STJA0038208

Anti-PP1C alpha antibody [1C11-C10-H5-E9] (STJA0038208)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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PPP1A antibody western blot HepG2 MCF-7 HeLa STJA0032376

Anti-PP1C alpha antibody [1H1-F7-D8] (STJA0032376)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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PPP1A antibody western blot C6 COS7 K562 3T3 HeLa STJA0038213

Anti-PP1C alpha antibody [1H1-F7-D8] (STJA0038213)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Monkey/Rat

$184.23 - $388.47

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Western blot analysis of various lysates using PPP1CA Rabbit pAb (STJ11106833) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-PPP1CA antibody (1-330 aa) (STJ11106833)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates, using PPP1CA antibody (STJ114342) at 1:800 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.

Anti-PPP1CA antibody (230-330) (STJ114342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PP1-alpha Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PPP1CA antibody (281-330 aa) (STJ95190)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis using PP1 Alpha monoclonal antibody against HepG2, MCF7, HeLa cell lysate.

Anti-PPP1CA antibody (N-Term) (STJ98533)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Cow/Chicken/Pig/Rabbit/Zebrafish

$205.62 - $496.80

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