Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of recombinant SARS-COV-2 N Protein, using SARS-CoV-2 N Protein Rabbit mAb, BSA and glycerol free (STJ11101210) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 30 ng/10 ng/5 ng/2 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-2019-nCoV antibody [S0MR] (STJ11101210)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IF/ICC/ELISA

Reactivity :

SARS-CoV-2

€158.13 - €693.13

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Western blot analysis of lysates from control 293T and SARS-CoV-2 Spike S1-293T trasfected cells, using SARS-CoV-2 Spike S1 mAb, BSA and glycerol free (STJ11101211) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-2019-nCoV antibody [S1MR] (STJ11101211)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

SARS-CoV-2

€158.13 - €693.13

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STJ72883 (2µg/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-5HT2A Receptor antibody (296-309) (STJ72883)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC/IF/FC

Reactivity :

Human

€390.63

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Confocal imaging of paraffin-embedded Mouse brain using 5HT7 Receptor Rabbit monoclonal antibody (STJ11103088, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citRate buffer (pH 6. 0) prior to IF staining.

Anti-5HT7 Receptor antibody (1-100) [S8MR] (STJ11103088)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Anti-6, 4DNA/6-4PPs antibody [64M-2] (STJA0018976)

Clonality :

Monoclonal

Host :

Mouse

Applications :

DB/ELISA/IF

€261.88 - €1,666.88

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Western blot analysis of various cells using A Cyclase V/VI Polyclonal Antibody

Anti-A Cyclase V/VI antibody (931-980 aa) (STJ91397)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of HeLa cells, using ADCY5/6 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-A Cyclase V/VI antibody (931-980 aa) (STJ91398)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-A2M antibody (1100-1180 aa) (STJ93980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from Hep G2 cells, using Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) ) Rabbit mAb (STJ11102540) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-A2M antibody (1200-1300) [S0MR] (STJ11102540)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Western blot analysis of AD293 cells using Macroglobulin Alpha-2 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-A2M antibody (871-920 aa) (STJ96733)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from K562 cells, using A4GNT Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-A4GNT antibody (31-80 aa) (STJ96365)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Anti-AACT antibody [SM4929] (STJA0004929)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

€291.88

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STJ71461 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-AADACL1 antibody (C-Term) (STJ71461)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human/Mouse/Dog/Cow

€390.63

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Immunohistochemistry analysis of AADAT in paraffin-embedded human liver using AADAT Rabbit polyclonal antibody (STJ115057) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-AADAT antibody (34-429) (STJ115057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various cells using AAK1 Polyclonal Antibody

Anti-AAK1 antibody (271-320 aa) (STJ91403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€61.25 - €326.25

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Western blot analysis of various cells using AASD-PPT Polyclonal Antibody

Anti-AASDHPPT antibody (11-60 aa) (STJ91405)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of mouse brain using GABA transaminase (GABA transaminase (ABAT) ) Rabbit polyclonal antibody (STJ27252) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ABAT antibody (29-300) (STJ27252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-ABCA1 antibody [SRM13184] (STJ11107914)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of paraffin-embedded mouse brain using ABCA2 Rabbit polyclonal antibody (STJ119146) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ABCA2 antibody (140-350) (STJ119146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Immunofluorescence analysis of U-251 MG cells using ABCA7 Rabbit polyclonal antibody (STJ11100236) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ABCA7 antibody (2010-2146) (STJ11100236)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various cells using ABCB10 Polyclonal Antibody

Anti-ABCB10 antibody (441-490 aa) (STJ91410)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from Mouse liver, using ABCB11 Rabbit pAb (STJ110765) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ABCB11 antibody (1050-1150) (STJ110765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Immunofluorescence analysis of NIH/3T3 cells using ABCB11 Rabbit polyclonal antibody (STJ116895) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ABCB11 antibody (1050-1150) (STJ116895)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various lysates using MRP2/ABCC2 Rabbit pAb (STJ110703) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ABCC2 antibody (180-1545) (STJ110703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Immunofluorescence analysis of paraffin-embedded Human pancreatic cancer tissue using MRP3 Rabbit polyclonal antibody (STJ11105616) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-ABCC3 antibody (855-954) (STJ11105616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

€138.13 - €631.88

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Western blot analysis of various lysates using ABCE1 Rabbit mAb (STJ11102433) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-ABCE1 antibody (500-599) [S3MR] (STJ11102433)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€148.13 - €651.88

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Anti-ABCE1/RNase L antibody (N-Term) (STJ71645)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

€390.63

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Immunofluorescence analysis of U2OS cells using ABCF2 antibody (STJ22464) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ABCF2 antibody (1-250) (STJ22464)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Immunofluorescence analysis of U2OS cells using ABCG2 Rabbit polyclonal antibody (STJ27628) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-ABCG2 antibody (100-200) (STJ27628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-ABCG2 antibody (289-338 aa) (STJ97515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Immunofluorescence analysis of human-breast-cancer tissue. 1, ABCG2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-ABCG2 antibody (461-510 aa) (STJ97253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis using ABCG2 monoclonal antibody against NIH/3T3 (1) and Cos7 (2) cell lysate.

Anti-ABCG2 antibody [3G8] (STJ97809)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Monkey

€186.25 - €450.00

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Anti-ABCG2 antibody [PT1012R] (STJA0040074)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-ABCG2 antibody [SRM14850] (STJ11109580)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human/Mouse

€0.00

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Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Anti-ABCG5 antibody (250-330 aa) (STJ190586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of HeLa cells using ABCG8 Rabbit polyclonal antibody (STJ22469) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ABCG8 antibody (1-320) (STJ22469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

€138.13 - €631.88

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Western blot analysis of various cells using ABHD12 Polyclonal Antibody

Anti-ABHD12 antibody (231-280 aa) (STJ91420)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various cells using ABHD2 Polyclonal Antibody

Anti-ABHD2 antibody (251-300 aa) (STJ91424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Immunofluorescence analysis of L929 cells using ABHD4 Rabbit polyclonal antibody (STJ11102928) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ABHD4 antibody (223-342) (STJ11102928)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various cells using ABHD6 Polyclonal Antibody

Anti-ABHD6 antibody (231-280 aa) (STJ91426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-ABHD6 antibody (Internal) (STJ71658)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

€390.63

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Western blot analysis of various cells using Abl1/2 Polyclonal Antibody

Anti-Abl1/2 antibody (406-455 aa) (STJ91432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of AD293 cells using Arg Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ABL2 antibody (601-650 aa) (STJ96723)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis using Arg monoclonal antibody against truncated ABL2 recombinant protein.

Anti-ABL2 antibody [1H1B11] (STJ97847)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

€180.00 - €435.00

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Western blot analysis of 3T3 cells using ACC Alpha Polyclonal Antibody

Anti-ACACA antibody (46-95 aa) (STJ91439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Cow/Dog

€61.25 - €326.25

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Western blot analysis of lysates from 1) Hela, 2) 293T, 3) NIH/3T3 cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-ACACA antibody (46-95 aa) (STJ91440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemistry analysis of paraffin-embedded human heart, using ACAD10 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ACAD10 antibody (231-280 aa) (STJ91433)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Immunofluorescence analysis of HeLa cells using ACADM Rabbit polyclonal antibody (STJ29869) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ACADM antibody (1-220) (STJ29869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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