Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of lysates from HeLa cells, using CAPRIN1 Rabbit pAb (STJ110219) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-CAPRIN1 antibody (1-709) (STJ110219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 900 Mu g extracts of A-549 cells using 3 Mu g CDK5 antibody (STJ28297). Western blot was performed from the immunoprecipitate using CDK5 antibody (STJ28297) at a dilution of 1:1000.

Anti-CDK5 antibody (1-292) (STJ28297)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of HepG2 cells using Cdk5 Polyclonal Antibody diluted at 1：500

Anti-CDK5 antibody (1-50 aa) (STJ92200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-CDK5 antibody [PT1229R] (STJA0040176)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-CDK5 antibody [SRM14897] (STJ11109627)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of human-lung tissue. 1, ERK 1/2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-ERK 1/2 antibody (330-379 aa) (STJ92990)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using ERK1/2 Mouse mAb diluted at 1:200.

Anti-ERK1/2 antibody (140-220 aa) [1H4] (STJ97783)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse/Fish

£49.00 - £261.00

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STJ70635 (0. 5µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ERK2/MAPK1 antibody (Internal) (STJ70635)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Immunofluorescence analysis of paraffin-embedded rat thymus using Fyn Rabbit polyclonal antibody (STJ11100097) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-FYN antibody (200-300) (STJ11100097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using Fyn Polyclonal Antibody diluted at 1：2000

Anti-FYN antibody (488-537 aa) (STJ93171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using Fyn monoclonal antibody against HeLa cell lysate.

Anti-FYN antibody [2A10] (STJ98091)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-Fyn antibody [PT0964R] (STJA0039838)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of various cells using GIMAP2 Polyclonal Antibody

Anti-GIMAP2 antibody (201-250 aa) (STJ93268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of 1) Mouse BrainTissue, 2) Rat Brain Tissue with KCNK9 Rabbit pAb diluted at 1:2,000.

Anti-KCNK9 antibody (220-270 aa) (STJ197366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of mouse brain, using MAP2 rabbit polyclonal antibody antibody (STJ114887) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-MAP2 antibody (1-118) (STJ114887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of MAP-2 antibody in paraffin-embedded human brain tissue.

Anti-MAP2 antibody (14-63 aa) (STJ94001)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of mouse brain cells using MAP2 Rabbit polyclonal antibody (STJ24462) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Map2 antibody (1510-1827) (STJ24462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis using MAP-2 monoclonal antibody against mouse brain, HeLa cell lysate.

Anti-MAP2 antibody (N-Term) (STJ98517)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Cow/Pig

£149.00 - £360.00

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Immunofluorescence analysis of Hela cell. 1, AQP2 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MAP2 antibody [7D4] (STJ96969)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-MAP2 antibody [PT0845R] (STJA0039198)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-MAP2 antibody [SRM14506] (STJ11109236)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of U2OS cells using MEK1 Rabbit polyclonal antibody (STJ24463). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP2K1 antibody (1-100) (STJ24463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain using 3 Mu g MEK1 antibody (STJ11101700). Western blot was performed from the immunoprecipitate using MEK1 (STJ11101700) at a dilution of 1:1000.

Anti-MAP2K1 antibody (1-100) [S0MR] (STJ11101700)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of MEK1 in (1) A431 lysates; (2) HeLa lysates using MEK1 antibody.

Anti-MAP2K1 antibody (1-40 aa) [R07-8C5] (STJA0015763)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat/Cow/Dog

£264.50

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Western blot analysis of 293T-UV cells using MEK-1 Polyclonal Antibody diluted at 1：500

Anti-MAP2K1 antibody (252-301 aa) (STJ94076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of U2OS cells using [KO Validated] MEK2 Rabbit polyclonal antibody (STJ116970) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MAP2K2 antibody (1-100) (STJ116970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of mouse-lung KB 293T lysis using MEK-2 antibody. Antibody was diluted at 1:2000

Anti-MAP2K2 antibody (261-310 aa) (STJ94081)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using MEK-2 monoclonal antibody against PC-12 (1) , Jurkat (2) , HeLa (3) and NIH/3T3 (4) cell lysate.

Anti-MAP2K2 antibody [7F5] (STJ98237)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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Immunofluorescence analysis of U-2 OS cells using MEK3 antibody (STJ110029) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MAP2K3 antibody (1-70) (STJ110029)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis using MEK-4 monoclonal antibody against HepG2 (1) , K562 (2) , and HEK293 (3) cell lysate.

Anti-MAP2K4 antibody [5H4] (STJ98239)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Immunofluorescence analysis of MCF7 cells using MEK5 Rabbit polyclonal antibody (STJ29033). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP2K5 antibody (1-190) (STJ29033)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 1) Hela, 2) 3T3, 3) PC12 using MEK-5 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MAP2K5 antibody (STJ197347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of U2OS cells using MEK6 antibody (STJ24469). Blue: DAPI for nuclear staining.

Anti-MAP2K6 antibody (1-334) (STJ24469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using MEK-6 Polyclonal Antibody

Anti-MAP2K6 antibody (1-50 aa) (STJ94087)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MAP2K6 antibody (150-230 aa) (STJ94088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using MEK-6 monoclonal antibody against HepG2 (1) , MCF-7 (2) and NIH/3T3 (3) cell lysate.

Anti-MAP2K6 antibody [3H12B9] (STJ98241)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£149.00 - £360.00

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Anti-MAP2K6 antibody [3H12C8] (STJ98240)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human/Mouse

£149.00 - £360.00

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Immunofluorescence analysis of L929 cells using MEK7 antibody (STJ24470) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MAP2K7 antibody (1-419) (STJ24470)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of 3T3 cells using ERK 2 Polyclonal Antibody diluted at 1：2000

Anti-MAPK1 antibody (136-185 aa) (STJ92992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of U-2 OS cells using [KO Validated] ERK2 Rabbit polyclonal antibody (STJ113009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAPK1 antibody (200-300) (STJ113009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (STJ24482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MAPK1 antibody (200-300) (STJ24482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Confocal imaging of HeLa cells using ERK1/2 Rabbit monoclonal antibody (STJ11102154, dilution 1:50) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-MAPK1/MAPK3 antibody (180-360) [S4MR] (STJ11102154)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of extracts of various cell lines, using ERK1/ERK2 antibody (STJ119100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAPK1/MAPK3 antibody (200-300) (STJ119100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Confocal immunofluorescence analysis of Hela cells using MAPK1/MAPK3 Rabbit polyclonal antibody (STJ119147) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-MAPK1/MAPK3 antibody (200-300) (STJ119147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using ERK1 antibody (STJ11101698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAPK3 antibody (1-100) [S8MR] (STJ11101698)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunohistochemistry analysis of ERK1 in paraffin-embedded mouse heart using [KD Validated] ERK1 Rabbit polyclonal antibody (STJ24487) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAPK3 antibody (1-378) (STJ24487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various cells using ERK 1 Polyclonal Antibody diluted at 1：500

Anti-MAPK3 antibody (161-210 aa) (STJ92987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of PC-12 using ERK1 Rabbit monoclonal antibody (STJ11104626) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAPK3 antibody (200-300) [S4626RM] (STJ11104626)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Rat

£126.50 - £554.50

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