Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of various cells using Cdk1/Cdc2 Polyclonal Antibody diluted at 1：2000

Anti-CDK1 antibody (101-150 aa) (STJ92194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of CDK1 in paraffin-embedded mouse brain using CDK1 Rabbit polyclonal antibody (STJ23048) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CDK1 antibody (198-297) (STJ23048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using CDK1 rabbit monoclonal antibody (STJ11101328) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CDK1 antibody (198-297) [S8MR] (STJ11101328)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£143.50 - £618.50

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Immunofluorescence analysis of human-stomach tissue. 1, Cdc2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-CDK1 antibody (5-54 aa) (STJ92154)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using CDC2 monoclonal antibody against Jurkat (1) cell lysate.

Anti-CDK1 antibody [8C5A7F10] (STJ97943)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-CDK1 antibody [SRM14524] (STJ11109254)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Cdk1/p34cdc2 antibody [POH-1] (STJ16101079)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Cow/Human/Mink/Monkey

£287.50 - £1,324.50

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Western blot analysis of extracts of various cell lines, using GFAP antibody (STJ11101596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GFAP antibody (1-100) [S6MR] (STJ11101596)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of mouse brain, using GFAP rabbit polyclonal antibody (STJ23770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GFAP antibody (1-75) (STJ23770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of Hela cell. 1, GFAP Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-GFAP antibody (11-60 aa) (STJ93259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-GFAP (ABT176) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Neuro-2aPredicted band size: 50kDaObserved band size: 57kDa

Anti-GFAP antibody (300-432 aa) [ABT176] (STJ197157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£180.00 - £261.00

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Immunofluorescence analysis of U-251MG cells using GFAP Rabbit polyclonal antibody (STJ116876) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-GFAP antibody (350-432) (STJ116876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of Hela cell. 1, AR Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GFAP monoclonal antibody (5C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-GFAP antibody [5C8] (STJ96961)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-GFAP antibody [N206B/9R] (STJA0019391)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC/WB

Reactivity :

Human

£209.50 - £1,333.50

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Anti-GFAP antibody [PT0298R] (STJA0039609)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-GFAP antibody [SAA0567] (STJA0019390)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IHC/WB

Reactivity :

Human

£209.50 - £1,333.50

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Western blot analysis of GFAP at dilution of 1:1000

Anti-GFAP antibody [SM0401] (STJA0010401)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£170.50 - £233.50

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Anti-GFAP antibody [SM0405] (STJA0010405)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£170.50 - £233.50

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Anti-GFAP antibody [SM0407] (STJA0010407)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£170.50 - £233.50

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Anti-GFAP antibody [SM5031] (STJA0005031)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Anti-GFAP antibody [SRM14921] (STJ11109651)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF-P

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-GFAP antibody [SRM14922] (STJ11109652)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF-P

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-GFAP antibody [SRM15065] (STJ11109795)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Mouse/Rat

£0.00

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Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T161 Rabbit pAb (STJ22061) at 1:400 dilution. HeLa cells were treated by EGF. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CDK1-T161 antibody (STJ22061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-CDK1-T161+CDK2/CDK3-T160-T161+T160 antibody [SRM15146] (STJ11109876)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-CDK1-Thr161 antibody (126-175 aa) (STJ91199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Cdc2 (T161) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CDK1-Thr161 antibody (131-180 aa) (STJ90213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-Y15 Rabbit pAb (STJ23048). HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-CDK1-Y15 antibody (STJ22059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various cells using Phospho-Cdk1/2/3 (T14) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-Cdk1/2/3-Thr14 antibody (1-50 aa) (STJ90219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using GFAP (Phospho-Ser38) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-GFAP-Ser38 antibody (11-60 aa) (STJ91108)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Recombinant-GFAP antibody [RM1P22] (STJA0023907)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human/Rat

£300.50 - £457.50

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Anti-Recombinant-GFAP antibody [SM0566] (STJA0010566)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£170.50 - £233.50

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Anti-Recombinant-GFAP antibody [SR0568] (STJA0010568)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£170.50 - £233.50

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Immunofluorescence analysis of Hela cell. 1, Rock-1 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-ROCK1 antibody (262-311 aa) (STJ95527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g ROCK1 antibody (STJ11103795). Western blot was performed from the immunoprecipitate using ROCK1 antibody (STJ11103795) at a dilution of 1:1000.

Anti-ROCK1 antibody (400-660) (STJ11103795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ROCK1 antibody [PT0305R] (STJA0039635)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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