Immunofluorescence Antibodies | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Acetyl-STAT3-Lys49 antibody (STJ193228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Akt2 Polyclonal Antibody diluted at 1：500

Anti-AKT2 antibody (432-481 aa) (STJ91552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis using Akt2 monoclonal antibody against A431 (1) , Jurkat (2) , HEK293 (3) , A549 (4) , MCF-7 (5) and PC-12 (6) cell lysate.

Anti-AKT2 antibody [1B6] (STJ97821)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Monkey

£149.00 - £360.00

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Anti-AKT2 antibody [SRM14707] (STJ11109437)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of PANC-1, using Ambra1 antibody (STJ22601) at 1:800 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-AMBRA1 antibody (1109-1208) (STJ22601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Confocal imaging of NIH/3T3 cells using [KO Validated] Cyclin D1 Rabbit monoclonal antibody (STJ11101576, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-CCND1 antibody (196-295) [S6MR] (STJ11101576)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells, using 3 Mu g Cyclin D1 antibody (STJ11101272). Western blot was performed from the immunoprecipitate using Cyclin D1 antibody (STJ11101272) at a dilution of 1:1000.

Anti-CCND1 antibody (200-295) (STJ11101272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of Hela cell. 1, Cyclin D1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CCND1 antibody (246-295 aa) (STJ92538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£49.00 - £261.00

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Immunofluorescence analysis of U-2 OS cells using p27 KIP 1 antibody (STJ23072) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CDKN1B antibody (1-198) (STJ23072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p27Kip1 (ABT-p27) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: MCF7Predicted band size: 22kDaObserved band size: 24kDa

Anti-CDKN1B antibody (100-198 aa) [ABT-p27] (STJ196998)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human

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Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, p27 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-CDKN1B antibody (149-198 aa) (STJ94866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse/Cow

£49.00 - £261.00

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Anti-CREPT antibody [3E10#] (STJA0019707)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IHC/WB

Reactivity :

Human

£209.50 - £1,333.50

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 3 Mu g Cullin 4A antibody (STJ23293). Western blot was performed from the immunoprecipitate using Cullin 4A antibody (STJ23293) at a dilution of 1:1000.

Anti-CUL4A antibody (1-300) (STJ23293)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of mouse liver, mouse kidney, mouse heart cells using CUL-4B Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CUL4B antibody (711-760 aa) (STJ96553)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using Cyclin D1 Rabbit pAb (STJ11102750) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Cyclin D1 antibody (200-295) (STJ11102750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of A549 cells, using ECRG4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ECRG4 antibody (41-90 aa) (STJ92822)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of MCF7 cells using PERK Rabbit polyclonal antibody (STJ11105581) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EIF2AK3 antibody (50-600) (STJ11105581)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunofluorescence analysis of NIH/3T3 cells using PERK Rabbit polyclonal antibody (STJ11100155) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EIF2AK3 antibody (867-1116) (STJ11100155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of Hela cell. 1, PERK Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-EIF2AK3 antibody (947-996 aa) (STJ95031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using eIF4E antibody (STJ23516) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-EIF4E antibody (1-100) (STJ23516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of MCF7 cells using eIF4E antibody (STJ114889). Blue: DAPI for nuclear staining.

Anti-EIF4E antibody (1-217) (STJ114889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of various cells using eIF4E Polyclonal Antibody diluted at 1：1000

Anti-EIF4E antibody (168-217 aa) (STJ92881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis using eIF4E monoclonal antibody against HeLa (1) , HEK293 (2) and K562 (3) cell lysate.

Anti-EIF4E antibody [5D11] (STJ98016)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-eIF4E antibody [PT0569R] (STJA0039248)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-eIF4E antibody [SRM14831] (STJ11109561)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

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Immunofluorescence analysis of L929 cells using FBXW8 Rabbit polyclonal antibody (STJ11100092) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-FBXW8 antibody (500-598) (STJ11100092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using Fhit Polyclonal Antibody

Anti-FHIT antibody (81-130 aa) (STJ93069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using GDF-9 Polyclonal Antibody

Anti-GDF9 antibody (273-322 aa) (STJ93256)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of U-2 OS cells using KIF14 Polyclonal Antibody (STJ112313) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-KIF14 antibody (1399-1648) (STJ112313)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using LEF1 antibody (STJ114908) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-LEF1 antibody (20-132) (STJ114908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ70898 (2µg/ml) staining of K562 nuclear cell lysate (A) + peptide (B). (35µg protein in RIPA buffer) Detected by chemiluminescence.

Anti-LEF1 antibody (Internal) (STJ70898)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Anti-LEF1 antibody [PT0601R] (STJA0039283)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunofluorescence analysis of NIH/3T3 cells using Leptin Rabbit polyclonal antibody (STJ111070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-LEP antibody (1-100) (STJ111070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human-placenta, antibody was diluted at 1:100

Anti-LEP antibody (10-50 aa) (STJ98979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NDRG2 antibody (10-90) (STJ94369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-PERK antibody [SRM15004] (STJ11109734)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-AKT2-S474 antibody [SRM13696] (STJ11108426)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Western blot analysis of various cells using Phospho-Akt2 (S474) Polyclonal Antibody diluted at 1：500

Anti-Phospho-AKT2-Ser474 antibody (432-481 aa) (STJ90169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Cyclin D1 (T286) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CCND1-Thr286 antibody (246-295 aa) (STJ90457)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-CDKN1B-Ser178 antibody (144-193 aa) (STJ91151)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Phospho-CDKN1B-Thr157 antibody (123-172 aa) (STJ91150)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-p27 (T187) Polyclonal Antibody

Anti-Phospho-CDKN1B-Thr187 antibody (149-198 aa) (STJ90443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, PERK (phospho Thr981) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-EIF2AK3-Thr981 antibody (947-996 aa) (STJ91215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-eIF4E (S209) Polyclonal Antibody

Anti-Phospho-EIF4E-Ser209 antibody (168-217 aa) (STJ90254)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-eIF4E-Ser209 antibody [PT1400R] (STJA0040395)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using FHIT (Phospho-Tyr114) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-FHIT-Tyr114 antibody (80-129 aa) (STJ91274)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-PKC Delta (S645) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PRKCD-Ser645 antibody (612-661 aa) (STJ91011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£49.00 - £261.00

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Western blot analysis of 3T3 cells using Phospho-PKC Delta (T507) Polyclonal Antibody

Anti-Phospho-PRKCD-Thr507 antibody (471-520 aa) (STJ90388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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