Immunofluorescence Antibodies | St John's Laboratory

Immunofluorescence analysis of U-2 OS cells using Acetyl-HMGB1-K29 Rabbit polyclonal antibody (STJ11102770) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Acetyl-HMGB1-K29 antibody (STJ11102770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of HepG2 cells treated with UV using Acetyl-HMG-1 (K12) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Acetyl-HMGB1-Lys12 antibody (STJ97227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of PANC-1, using Ambra1 antibody (STJ22601) at 1:800 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-AMBRA1 antibody (1109-1208) (STJ22601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of U2OS cells using ATG14 Rabbit polyclonal antibody (STJ29664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG14 antibody (340-440) (STJ29664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-ATG14 antibody (STJ197438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-ATG14 antibody [PT1393R] (STJA0040026)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunofluorescence analysis of HeLa cells using Ataxin-3 (ATXN3) antibody (STJ114961). Blue: DAPI for nuclear staining.

Anti-ATXN3 antibody (1-361) (STJ114961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunofluorescence analysis of U2OS cells using Ataxin-3 (ATXN3) Rabbit polyclonal antibody (STJ22739) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATXN3 antibody (1-361) (STJ22739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using Bcl-2 antibody (STJ11103448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Bcl 2 antibody [S8MR] (STJ11103448)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, Dnmt3b Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Bcl-2 monoclonal antibody (6B5) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-BCL2 antibody (1-100 aa) [6B5] (STJ96943)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Mouse/Rat/Chicken

£49.00 - £261.00

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Immunoprecipitation analysis of 200 Mu g extracts of THP-1 cells using 3 Mu g Bcl-2 antibody (STJ27524). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (STJ27524) at a dilution of 1:1000.

Anti-BCL2 antibody (1-100) (STJ27524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of L929 cells using Bcl-2 Rabbit polyclonal antibody (STJ113693) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BCL2 antibody (1-239) (STJ113693)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from 1) mouse-liver, 2) mouse-brain, 3) mouse-lung, 4) mcf-7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-BCL2 antibody (46-95 aa) (STJ91835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

£49.00 - £261.00

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Anti-Beclin 1 antibody [SRM13210] (STJ11107940)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Beclin-1 Mouse mAb diluted at 1:200.

Anti-BECN1 antibody (110-190 aa) [5C2] (STJ97761)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse/Cow

£49.00 - £261.00

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Anti-BECN1 antibody (STJA0032127)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£406.50

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Western blot analysis using Beclin-1 monoclonal antibody against HeLa (1) , A431 (2) , MCF-7 (3) , RAJI (4) , Jurkat (5) and SKBR-3 (6) cell lysate.

Anti-BECN1 antibody [2A4] (STJ97862)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Immunofluorescence analysis of NIH/3T3 cells using BOK Rabbit polyclonal antibody (STJ118782) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-BOK antibody (1-100) (STJ118782)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-cGAS antibody [PT0355R] (STJA0039574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£285.00 - £540.00

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Anti-cGAS antibody [PT0700R] (STJA0039268)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Mouse

£150.00 - £540.00

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Western blot analysis ofmouse braincells using DAPK1 Polyclonal Antibody diluted at 1：1000

Anti-DAPK1 antibody (274-323 aa) (STJ92652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry of paraffin embedded human amygdalitis using MAC-2 at dilution of 1:1000

Anti-Galectin 3 antibody [SM0471] (STJA0010471)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Western blot analysis of Galectin-3 at dilution of 1:1000

Anti-Galectin 3 antibody [SM4925] (STJA0004925)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat

£170.50 - £233.50

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Anti-Galectin 3/LGALS3 antibody [SRM13238] (STJ11107968)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-Galectin 3/LGALS3 antibody [SRM13469] (STJ11108199)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/FC/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Galectin 3/LGALS3 antibody [SRM14575] (STJ11109305)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunofluorescence analysis of HeLa cells using GRB2 antibody (STJ27656). Blue: DAPI for nuclear staining.

Anti-GRB2 antibody (1-217) (STJ27656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunoprecipitation analysis of 300 Mu g extracts from 293T cells using 3 Mu g GRB2 antibody (STJ11101597). Western blot was performed from the immunoprecipitate using GRB2 (STJ11101597) at a dilution of 1:1000.

Anti-GRB2 antibody (118-217) [S7MR] (STJ11101597)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-GRB2 antibody [PT1228R] (STJA0040137)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of extracts of various cell lines, using HMGB1 antibody (STJ11101678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HMGB1 antibody (100-200) [S8MR] (STJ11101678)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, HMG-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HMGB1 antibody (N-Term) (STJ96867)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-HMGB1 antibody [DPH11] (STJA0019032)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/FuncS/IF/WB

Reactivity :

Human/Mouse/Rat/Bovine/Dog/Horse/Pig

£209.50 - £1,333.50

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Anti-HMGB1 antibody [PT0499R] (STJA0039474)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-JNK1 antibody [SRM14610] (STJ11109340)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of various cells using JNK1/2/3 Polyclonal Antibody diluted at 1：1000

Anti-JNK1/2/3 antibody (166-215 aa) (STJ93805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Chicken/Fish/Pig

£49.00 - £261.00

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Immunofluorescence analysis of U-2 OS cells using [KO Validated] Galectin 3/LGALS3 Rabbit polyclonal antibody (STJ115467) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-LGALS3 antibody (1-250) (STJ115467)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, CBP 35 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-LGALS3 antibody (141-190 aa) (STJ92060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 1) MCF7, 2) 3T3 with Galectin-3 Mouse mAb diluted at 1:2, 000.

Anti-LGALS3 antibody [8D7] (STJ97550)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-LGALS3 antibody [P3] (STJA0027508)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rabbit

£254.50 - £329.50

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Anti-LGALS3 antibody [P3] {APC} (STJA0029244)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC/IF/WB

Reactivity :

Human/Mouse/Rabbit

£310.50 - £405.50

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Anti-LGALS3 antibody [P3] {FITC} (STJA0028278)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC/IF/WB

Reactivity :

Human/Mouse/Rabbit

£254.50 - £329.50

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Anti-LGALS3 antibody [P3] {PerCP} (STJA0030210)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC/IF/WB

Reactivity :

Human/Mouse/Rabbit

£292.50 - £379.50

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Anti-LGALS3 antibody [P3] {PE} (STJA0031176)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC/IF/WB

Reactivity :

Human/Mouse/Rabbit

£310.50 - £405.50

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Immunofluorescence analysis of HeLa cells using JNK1 antibody (STJ24490) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAPK8 antibody (245-345) (STJ24490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot detection of JNK1 in C6 and 3T3 cell lysates using JNK1 mouse mAb (1:1000 diluted).Predicted band size:46, 54KDa.Observed band size:46KDa.

Anti-MAPK8 antibody [1A4-C5-F11] (STJ99225)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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Western blot analysis of lysates from HeLa cells using cGAS Rabbit pAb (STJ110633) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-MB21D1 antibody (350-522) (STJ110633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ70753 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-MEFV antibody (Internal) (STJ70753)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

£312.50

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Western blot analysis of lysates from Jurkat and 293 cells, using Phospho-Bcl-2-S70 Rabbit pAb (STJ29341) at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight. 293 cells were treated by UV for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-BCL2-S70 antibody (STJ29341)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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