Immunofluorescence Antibodies | St John's Laboratory

Confocal immunofluorescence analysis of HeLa cells using [KO Validated] AIF Rabbit polyclonal antibody (STJ22557) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-AIFM1 antibody (334-613) (STJ22557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various cells using AIF-M1 Polyclonal Antibody diluted at 1：1000

Anti-AIFM1 antibody (51-100 aa) (STJ91514)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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STJ73128 (0. 01µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AIFM1 antibody (C-Term) (STJ73128)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Western blot analysis using AIF-M1 monoclonal antibody against NIH/3T3 (1) , Jurkat (2) , HeLa (3) , HepG2 (4) , MOLT4 (5) , C6 (6) , RAJI (7) , Cos7 (8) and PC-12 (9) cell lysate.

Anti-AIFM1 antibody [4E7] (STJ97819)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£149.00 - £360.00

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Western blot analysis of extracts from Hela, Ramos, HepG2, MCF7 and Jurkat cell lysates using AIF mouse mAb (1:1000 diluted).Predicted band size:67KDa.Observed band size:67KDa.

Anti-AIFM1 antibody [8H1-B10-A12] (STJ99301)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human

£149.00 - £360.00

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Anti-AIFM1 antibody [PT0650R] (STJA0039304)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of various cells using AMID Polyclonal Antibody

Anti-AIFM2 antibody (141-190 aa) (STJ91575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£49.00 - £261.00

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Western blot analysis of various cells using AIFL Polyclonal Antibody

Anti-AIFM3 antibody (10-59 aa) (STJ91513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of A-431 cells using AIM2 Rabbit polyclonal antibody (STJ22558) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-AIM2 antibody (130-337) (STJ22558)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of Hela cell. 1, AIM2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HSP70 monoclonal antibody (3G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-AIM2 antibody (51-100 aa) (STJ91516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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STJ72169 (1µg/ml) staining of IFNbeta-treated WI-38 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AIM2 antibody (C-Term) (STJ72169)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human

£312.50

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Anti-AIM2 antibody [PT1057R] (STJA0039969)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunofluorescence analysis of C6 cells using AIRE Rabbit polyclonal antibody (STJ22559) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AIRE antibody (30-130) (STJ22559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using AIRE-1 Polyclonal Antibody diluted at 1：1000

Anti-AIRE antibody (91-140 aa) (STJ91518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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STJ70203 (1µg/ml) staining of nuclear Jurkat cell (A) , (2ug/ml) Human Spleen (B) and negative control Human Smooth Muscle (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AIRE antibody (C-Term) (STJ70203)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human

£312.50

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Anti-AK2 antibody (1-239aa) [AT7E7] (STJA0041768)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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Immunocytochemistry analysis of AK2 (green) in Hela using AK2 antibody, and DAPI (blue).

Anti-AK2 antibody (171-184 aa) [R07-4H8] (STJA0012261)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-AK2 antibody [SRM13117] (STJ11107847)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-AK3 antibody [SJB3-36] (STJA0041462)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/IHC/FACS

Reactivity :

Human

£219.50 - £332.50

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Anti-AK3L1 antibody [R08-2B2] (STJA0035110)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Immunofluorescence analysis of NIH/3T3 cells, using AKAP3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-AKAP3 antibody (191-240 aa) (STJ91525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-AKAP9 antibody (2610-2690 aa) (STJ190247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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STJ70660 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Anti-AKAP9/AKAP450/CG-NAP antibody (C-Term) (STJ70660)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Mouse

£312.50

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Western blot analysis of various cells using BCA3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-AKIP1 antibody (111-160 aa) (STJ91827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells using AKR1A1 antibody (STJ115538).

Anti-AKR1A1 antibody (1-325) (STJ115538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of PC-12 cells using AKR1B1 Rabbit polyclonal antibody (STJ22567) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-AKR1B1 antibody (1-316) (STJ22567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using Aldose Reductase Polyclonal Antibody

Anti-AKR1B1 antibody (241-290 aa) (STJ91559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£49.00 - £261.00

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Anti-AKR1B1 antibody [SRM14490] (STJ11109220)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-AKR1C1 antibody (30-110 aa) (STJ92669)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of U-2 OS cells using AKR1C2 Polyclonal Antibody (STJ116408) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AKR1C2 antibody (1-323) (STJ116408)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of COLO cells using DD2 Polyclonal Antibody

Anti-AKR1C2 antibody (21-70 aa) (STJ92670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunoprecipitation analysis of 200 Mu g extracts of K-562 cells, using 3 Mu g AKR1C3 antibody (STJ22568). Western blot was performed from the immunoprecipitate using AKR1C3 antibody (STJ22568) at a dilution of 1:1000.

Anti-AKR1C3 antibody (1-323) (STJ22568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Confocal imaging of A549 cells using AKR1C3 Rabbit monoclonal antibody (STJ11101962, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-AKR1C3 antibody (200-300) [S2MR] (STJ11101962)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-AKR1C3 antibody [R08-8G6] (STJA0036876)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Human

£133.50 - £281.50

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Anti-AKR7A2 antibody [R03-5C-3] (STJA0033082)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human

£214.50 - £308.50

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Immunofluorescence analysis of human-stomach tissue. 1, Akt Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Akt antibody (281-330 aa) (STJ91550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

£49.00 - £261.00

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Anti-AKT antibody [R08-8F1] (STJA0033993)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Akt1 antibody (1-480aa) [AT6D5] (STJA0041853)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

£219.50 - £332.50

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Western blot analysis of extracts of various cell lines, using AKT1 antibody (STJ27474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-AKT1 antibody (100-200) (STJ27474)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of MCF7 cells, using AKT1 antibody (STJ112889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-AKT1 antibody (350-480) (STJ112889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Akt Mouse mAb diluted at 1:200.

Anti-AKT1 antibody (370-477 aa) [10D6] (STJ97724)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat/D.melanogaster

£49.00 - £261.00

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Confocal imaging of MCF7 cells using [KO Validated] AKT1 Rabbit monoclonal antibody (STJ11104954, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-AKT1 antibody (380-480) [S4954RM] (STJ11104954)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from 1) 3T3, 2) 293T, 3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-AKT1 antibody (400-480 aa) (STJ91540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of human-lung tissue. 1, Akt1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-AKT1 antibody (95-144 aa) (STJ91542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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STJ73595 (0. 3µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AKT1 antibody (Internal) (STJ73595)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse

£312.50

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Anti-AKT1 antibody [R03-5K6] (STJA0035720)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-AKT1 nanobody [SAA1231] (STJN000197)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

IF

Reactivity :

Human

£195.50 - £1,319.50

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Anti-AKT1-E49K Mutant antibody (STJA0033552)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/ELISA

Reactivity :

Vertebrates

£349.50 - £519.50

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