• Western blot analysis of extracts of various cell lines, using ACKR3 rabbit polyclonal antibody (STJ114585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of mouse skin using ACKR3 rabbit polyclonal antibody (STJ114585) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ACKR3 antibody (150-250) (STJ114585)

SKU:
STJ114585

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACKR3 (150-250) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACKR3
Gene ID: 57007
Uniprot ID: ACKR3_HUMAN
Immunogen Region: 150-250
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ACKR3 (NP_064707.1).
Immunogen Sequence: TNTPSSRKKMVRRVVCILVW LLAFCVSLPDTYYLKTVTSA SNNETYCRSFYPEHSIKEWL IGMELVSVVLGFAVPFSIIA VFYFLLARAISASSDQEKHS S
Tissue Specificity Expressed in monocytes, basophils, B-cells, umbilical vein endothelial cells (HUVEC) and B-lymphoblastoid cells. Lower expression detected in CD4+ T-lymphocytes and natural killer cells. In the brain, detected in endothelial cells and capillaries, and in mature neurons of the frontal cortex and hippocampus. Expressed in tubular formation in the kidney. Highly expressed in astroglial tumor endothelial, microglial and glioma cells. Expressed at low levels in normal CD34+ progenitor cells, but at very high levels in several myeloid malignant cell lines. Expressed in breast carcinomas but not in normal breast tissue (at protein level).
Post Translational Modifications The Ser/Thr residues in the C-terminal cytoplasmic tail may be phosphorylated. Ubiquitinated at the Lys residues in its C-terminal cytoplasmic tail and is essential for correct trafficking from and to the cell membrane. Deubiquitinated by CXCL12-stimulation in a reversible manner.
Function Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1. Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival. Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration. Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12. Required for heart valve development. Regulates axon guidance in the oculomotor system through the regulation of CXCL12 levels. (Microbial infection) Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.
Protein Name Atypical Chemokine Receptor 3
C-X-C Chemokine Receptor Type 7
Cxc-R7
Cxcr-7
Chemokine Orphan Receptor 1
G-Protein Coupled Receptor 159
G-Protein Coupled Receptor Rdc1 Homolog
Rdc-1
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Early Endosome
Recycling Endosome
Predominantly Localizes To Endocytic Vesicles
And Upon Stimulation By The Ligand Is Internalized Via Clathrin-Coated Pits In A Beta-Arrestin-Dependent Manner
Once Internalized
The Ligand Dissociates From The Receptor
And Is Targeted To Degradation While The Receptor Is Recycled Back To The Cell Membrane
Alternative Antibody Names Anti-Atypical Chemokine Receptor 3 antibody
Anti-C-X-C Chemokine Receptor Type 7 antibody
Anti-Cxc-R7 antibody
Anti-Cxcr-7 antibody
Anti-Chemokine Orphan Receptor 1 antibody
Anti-G-Protein Coupled Receptor 159 antibody
Anti-G-Protein Coupled Receptor Rdc1 Homolog antibody
Anti-Rdc-1 antibody
Anti-ACKR3 antibody
Anti-CMKOR1 antibody
Anti-CXCR7 antibody
Anti-GPR159 antibody
Anti-RDC1 antibody

Information sourced from Uniprot.org

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