Anti-Acetyl-RELA-K314/315 antibody (STJ98906)


Current Stock:
Host: Mouse
Applications: IHC-P, IF-P
Reactivity: Human, Mouse, Rat
Short Description: Mouse monoclonal antibody anti-Acetyl-Transcription Factor P65-K314/315 is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Conjugation: Unconjugated
Formulation: PBS, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was isolated from ascitic fluid by immunoaffinity chromatography using antigens coupled to agarose beads.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RELA
Gene ID: 5970
Uniprot ID: TF65_HUMAN
Specificity: Acetyl-RELA-K314/315monoclonal antibody (Transcription Factor P65) binds to endogenous Transcription Factor P65.
Immunogen: Synthetic peptide of Acetyl NF kB P65 (K314/K315)
Function NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells. The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection.
Protein Name Transcription Factor P65
Nuclear Factor Nf-Kappa-B P65 Subunit
Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3
Database Links Reactome: R-HSA-1169091
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Reactome: R-HSA-9660826
Cellular Localisation Nucleus
But Also Found In The Cytoplasm In An Inactive Form Complexed To An Inhibitor (I-Kappa-B)
Colocalized With Ddx1 In The Nucleus Upon Tnf-Alpha Induction
Colocalizes With Gfi1 In The Nucleus After Lps Stimulation
Translocation To The Nucleus Is Impaired In L
Monocytogenes Infection
Alternative Antibody Names Anti-Transcription Factor P65 antibody
Anti-Nuclear Factor Nf-Kappa-B P65 Subunit antibody
Anti-Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3 antibody
Anti-RELA antibody
Anti-NFKB3 antibody

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