• STJ70663 (0. 3µg/ml) staining of Jurkat (A) and (0. 5ug/ml) HepG2 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70663 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear, membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70663 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-ACHE antibody (C-Term) (STJ70663)

SKU:
STJ70663-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ACHE (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IF-Strong expression of the protein seen in the cytoplasm and nucleus of U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ACHE
Gene ID: 43
Uniprot ID: ACES_HUMAN
Immunogen Region: C-Term
Accession Number: NP_000656.1; NP_001269378.1
Specificity: This antibody is expected to recognise isoform NP_000656.1 only (the ubiquitously expressed, hydrophillic form).
Immunogen Sequence: QFDHYSKQDRCSDL
Function Hydrolyzes rapidly the acetylcholine neurotransmitter released into the synaptic cleft allowing to terminate the signal transduction at the neuromuscular junction. Role in neuronal apoptosis.
Protein Name Acetylcholinesterase
Ache
Database Links Reactome: R-HSA-112311
Reactome: R-HSA-1483191
Reactome: R-HSA-422085
Cellular Localisation Synapse
Secreted
Cell Membrane
Peripheral Membrane Protein
Isoform T: Nucleus
Only Observed In Apoptotic Nuclei
Isoform H: Cell Membrane
Lipid-Anchor
Gpi-Anchor
Extracellular Side
Alternative Antibody Names Anti-Acetylcholinesterase antibody
Anti-Ache antibody
Anti-ACHE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance