Immunofluorescence Antibodies | St John's Laboratory

Phospho-Vimentin Ser56 antibody immunocytochemistry HeLa STJA0034994

Anti-Phospho-Vimentin-Ser56 antibody [R01-0E2] (STJA0034994)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of 3T3 cells using Phospho-WAVE1 (Y125) Polyclonal Antibody

Anti-Phospho-WASF1-Tyr125 antibody (91-140 aa) (STJ90840)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat cells treated with Ca+ 40nM 30', using ZAP-70 (Phospho-Tyr315) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ZAP70-Tyr315 antibody (281-330 aa) (STJ90842)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat cells, using ZAP-70 (Phospho-Tyr319) Antibody. The lane on the right is STJ90436

Anti-Phospho-ZAP70-Tyr319 antibody (286-335 aa) (STJ90436)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat cells, using ZAP-70 (Phospho-Tyr493) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ZAP70-Tyr493 antibody (460-509 aa) (STJ90437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Confocal imaging of EL4 cells (treated with H‚‚O‚‚ and pervanadate) and EL4 cells (untreated) using Phospho-Zap70-Y319+Syk-Y352 Rabbit pAb (STJ11107734, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-Phospho-Zap70-Y319 + Y352 antibody (STJ11107734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse

£126.50 - £618.50

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Western blot analysis of lysates from Jurkat cells, using Phospho-ZAP70-Y493 Rabbit pAb (STJ22449) at 1:1000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37 °C for 2 minutes after serum-starvation overnight or treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-ZAP70-Y493 antibody (STJ22449)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of Jurkat treated with H2O2 and Jurkat using Phospho-ZAP70-Y493 Rabbit monoclonal antibody (STJ11105888) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Phospho-ZAP70-Y493 antibody [S5888RM] (STJ11105888)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Phospho-lipase C gamma 1 antibody immunocytochemistry HeLa STJA0035321

Anti-Phospholipase C gamma 1 antibody [R01-6B4] (STJA0035321)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Immunofluorescence analysis of human-lung tissue. 1, PI 3-kinase p85 Alpha/Gamma Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-PI 3-kinase p85 Alpha/Gamma antibody (436-485 aa) (STJ95078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Chicken

£49.00 - £261.00

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PI3 Kinase p110 alpha antibody western blot Jurkat STJA0035836

Anti-PI3 Kinase p110 alpha antibody [R02-6C5] (STJA0035836)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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PI3 Kinase p55 gamma antibody western blot HEK-293T 3T3 C6 STJA0035941

Anti-PI3 Kinase p55 gamma antibody [R08-4G4] (STJA0035941)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunofluorescence analysis of U2OS cells using PIK3CA Rabbit polyclonal antibody (STJ24991) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PIK3CA antibody (401-1068) (STJ24991)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using PI3 Kinase p85 alpha Rabbit pAb (STJ113725) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-PIK3R1 antibody (240-724) (STJ113725)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using primary antibody diluted at 1:500 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-PIK3R1 antibody (550-630 aa) (STJ99654)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£49.00 - £261.00

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Western blot analysis of various cells using PI 3-kinase p85 Alpha Polyclonal Antibody diluted at 1：1000

Anti-PIK3R1 antibody (573-622 aa) (STJ95077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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PI3 Kinase p85 alpha antibody western blot Jurkat Rat Brain C6 CHO-K1 STJA0013015

Anti-PIK3R1 antibody (601-619 aa) [R03-3C7] (STJA0013015)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat/Hamster

£133.50 - £281.50

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Immunohistochemical analysis of paraffin-embedded Human Pancreas Carcinoma Tissue using PI3 Kinase P85 Rabbit pAb diluted at 1:500.

Anti-PIK3R1 antibody (STJ197340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of 1) 3T3, 2) Rat LiverTissue with PI3 Kinase P85 Alpha Mouse mAb diluted at 1:2, 000.

Anti-PIK3R1 antibody [3B7] (STJ97521)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Rat/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human-testis, antibody was diluted at 1:100

Anti-PIK3R2 antibody (1-60 aa) (STJ98987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of NIH/3T3 using PI3 Kinase p85 beta Rabbit polyclonal antibody (STJ119550) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PIK3R2 antibody (1-85) (STJ119550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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PI3 kinase p85 beta antibody western blot HeLa STJA0014501

Anti-PIK3R2 antibody (140-164 aa) [R06-5H3] (STJA0014501)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Rat

£133.50 - £281.50

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Immunofluorescence analysis of NIH-3T3 cells using PIK3R3/p55PIK Rabbit polyclonal antibody (STJ11101208) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PIK3R3 antibody (300-388) (STJ11101208)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PIK3R3 antibody (300-388) (STJ119452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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PIM1 antibody western blot Jurkat STJA0035962

Anti-PIM1 antibody [R07-7J8] (STJA0035962)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Immunofluorescence analysis of A549 cells using PIP4K2A antibody (STJ29582).

Anti-PIP4K2A antibody (120-350) (STJ29582)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ70529 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and Endoplasmic Reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-PITPNM/PITPNM1 antibody (C-Term) (STJ70529)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human/Mouse/Dog/Cow

£312.50

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Western blot analysis of various cells using PKA Alpha/Beta cat Polyclonal Antibody diluted at 1：1000

Anti-PKA Alpha/Beta cat antibody (1-50 aa) (STJ95113)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, PKA Alpha/Beta/Gamma cat Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-PKA Alpha/Beta/Gamma cat antibody (166-215 aa) (STJ95114)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£49.00 - £261.00

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Western blot analysis of various lysates, using PKA C-alpha (PRKACA) antibody (STJ11102940) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-PKA C-alpha antibody [S0MR] (STJ11102940)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-PKC delta antibody [R09-8H6] (STJA0034731)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£140.50 - £308.50

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PKD2 antibody western blot HeLa lysates STJA0036795

Anti-PKD2 antibody [R05-3O3] (STJA0036795)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse

£133.50 - £281.50

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ICC IF of PKM2 in HeLa cells stained with STJA0041705 antibody Alexa Fluor 488 secondary green and DAPI nuclear stain blue

Anti-PKM antibody (1-531aa) [AT1B10] (STJA0041705)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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Immunofluorescence analysis of U2OS cells using PKM1/2 Rabbit polyclonal antibody (STJ115844) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PKM antibody (11-221) (STJ115844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using PKM2 Rabbit pAb (STJ25012) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PKM antibody (11-221) (STJ25012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of rat-lung tissue. 1, PKM2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-PKM antibody (181-230 aa) (STJ95142)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-PKM antibody (301-350 aa) (STJ193177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot detection of PKM2 in COS7, PC12, C6, 3T3 and Hela cell lysates using PKM2 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.

Anti-PKM antibody [1A7-G6-H6] (STJ99145)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Monkey

£149.00 - £360.00

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Western blot detection of PKM2 in HCT116, COS7, K562, A549 and Hela cell lysates using PKM2 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.

Anti-PKM antibody [1F2-F4-H11] (STJ99144)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse/Monkey

£149.00 - £360.00

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Anti-PKM antibody [PT0080R] (STJA0039734)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of extracts of various cell lines, using PKM1-specific antibody (STJ11101494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PKM1-specific antibody (350-450) (STJ11101494)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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PKM2 antibody western blot COS7 C6 3T3 HeLa STJA0035704

Anti-PKM2 antibody (STJA0035704)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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PKM2 antibody western blot COS7 PC-12 C6 3T3 HeLa STJA0033205

Anti-PKM2 antibody [1A7-G6-H6] (STJA0033205)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat/Monkey

£133.50 - £281.50

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PKM2 antibody western blot HCT116 COS7 K562 A549 HeLa STJA0032405

Anti-PKM2 antibody [1F2-F4-H11] (STJA0032405)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Monkey

£133.50 - £281.50

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PKM2 antibody western blot HeLa rat mouse skeletal muscle STJA0036088

Anti-PKM2 antibody [R09-7E7] (STJA0036088)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunofluorescence analysis of NIH-3T3 cells using PKM2-specific Rabbit polyclonal antibody (STJ11101493) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PKM2-specific antibody (380-480) (STJ11101493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using Myt 1 Polyclonal Antibody diluted at 1：2000

Anti-PKMYT1 antibody (49-98) (STJ94323)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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PKR antibody immunocytochemistry 3T3 cells STJA0033868

Anti-PKR antibody [R04-3F2] (STJA0033868)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Mouse/Rat

£133.50 - £281.50

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