Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of various lysates, using AR-V7 Rabbit polyclonal antibody (STJ11105166) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AR-V7 antibody (544-644) (STJ11105166)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$152.49 - $697.59

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Anti-ARA70 antibody [PT1066R] (STJA0040346)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Western blot analysis of various cells using A-Raf Polyclonal Antibody

Anti-ARAF antibody (276-325 aa) (STJ91668)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of HeLa cells using ARAF antibody (STJ118774) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ARAF antibody (371-606) (STJ118774)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using ARAP1 antibody (STJ112495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-ARAP1 antibody (834-1133) (STJ112495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

$152.49 - $697.59

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Anti-ARC antibody [PT1315R] (STJA0040251)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Immunofluorescence analysis of C6 cells using ARCN1 Rabbit polyclonal antibody (STJ117191) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARCN1 antibody (212-511) (STJ117191)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-AREB6 antibody [PT0216R] (STJA0039659)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

$207.00 - $745.20

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STJ71831 (0. 05µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AREB6/ZEB1 antibody (Internal) (STJ71831)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Mouse/Rat

$431.25

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Anti-AREB6/ZEB1 antibody [8B12D7] (STJ16103610)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC/ICC/WB

Reactivity :

Human/Mouse/Rat

$1,030.17

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Immunofluorescence analysis of NIH-3T3 cells using AREG Rabbit polyclonal antibody (STJ114553) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AREG antibody (20-252) (STJ114553)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse

$152.49 - $697.59

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Anti-ARF1 antibody (1-181aa) [AT1B3] (STJA0041609)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

$302.91 - $458.85

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Western blot analysis of various lysates using ARF1 Rabbit mAb (STJ11102451) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-ARF1 antibody (70-150) [S1MR] (STJ11102451)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-ARF1 antibody (STJ500153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IP/WB

Reactivity :

Human/Mouse/Rat

$605.13

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Anti-ARF1 antibody {Biotin} (STJ500154)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-ARF1 antibody {FITC} (STJ500155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Immunofluorescence analysis of NIH/3T3 cells using ARF4 Rabbit polyclonal antibody (STJ117836) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARF4 antibody (1-180) (STJ117836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARF4 antibody (71-120 aa) (STJ91673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Confocal imaging of NIH/3T3 cells using ARF5 Rabbit monoclonal antibody (STJ11101858, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-ARF5 antibody (81-180) [S8MR] (STJ11101858)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunofluorescence analysis of HeLa cells using ARF6 antibody (STJ22670) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ARF6 antibody (1-175) (STJ22670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of C6 cells using ARF6 Rabbit monoclonal antibody (STJ11101343) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ARF6 antibody (76-175) [S3MR] (STJ11101343)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-ARF6 antibody [R02-5C5] (STJA0036289)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARFGAP1 antibody (171-220 aa) (STJ91671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Cow

$67.62 - $360.18

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Immunofluorescence analysis of U-2 OS cells using ARFGAP2 Rabbit polyclonal antibody (STJ119821) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARFGAP2 antibody (242-521) (STJ119821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various cells using BIG2 Polyclonal Antibody

Anti-ARFGEF2 antibody (1491-1540 aa) (STJ91854)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded Human thymus gland. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARFIP1 antibody (271-320 aa) (STJ91675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-ARFRP1/ARP antibody [SRM13096] (STJ11107826)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of paraffin-embedded human liver cancer using Arginase 1 (ARG1) Rabbit polyclonal antibody (STJ26182) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ARG1 antibody (1-322) (STJ26182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of 293 cells using Arginase I Polyclonal Antibody

Anti-ARG1 antibody (61-110 aa) (STJ91676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-ARG2/Arginase II antibody [C0020187] (STJA0019245)

Clonality :

Monoclonal

Host :

Mouse

Applications :

BIF/IF

Reactivity :

Human

$289.11 - $1,840.23

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Anti-Arginase 1 (ARG1) antibody [SRM15100] (STJ11109830)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Anti-Arginase 2 antibody [R06-7F9] (STJA0037396)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human

$184.23 - $388.47

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Anti-Arginase 2/ARG2 antibody (23-354aa) [AT7F8] (STJA0041764)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

$302.91 - $458.85

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STJ70719 (0. 01µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Arginase I antibody (C-Term) (STJ70719)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

$431.25

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STJ71134 (0. 05µg/ml) staining of Mouse Liver (A) and Rat Liver (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Arginase, type 1/arg1 (rat)-Rat antibody (C-Term) (STJ71134)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Mouse/Rat

$431.25

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STJ71133 (0. 01µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Arginase, type 1/ARG1 antibody (C-Term) (STJ71133)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Dog

$431.25

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Anti-Arginase-1 antibody (200-322) [ABT-Arg1] (STJA0039040)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF/ELISA

Reactivity :

Human

$67.62 - $360.18

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Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of paraffin-embedded mouse liver using Arginase-1 Rabbit polyclonal antibody (STJ11105172) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Arginase-1 antibody (STJ11105172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

$152.49 - $697.59

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Anti-Arginase-1 antibody [PT0364R] (STJA0039493)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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STJ71128 (0. 3µg/ml) staining of A431 (A) and (1ug/ml) NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Argininosuccinate synthetase 1 antibody (Internal) (STJ71128)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC/IHC

Reactivity :

Human/Mouse/Rat/Dog/Cow

$431.25

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Anti-Argonaute 2 antibody [R09-8P8] (STJA0036275)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-Argonaute-2 antibody [SRM14835] (STJ11109565)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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STJ70389 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Anti-ARH/LDL receptor adaptor antibody (N-Term) (STJ70389)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

$431.25

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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using RHG1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ARHGAP1 antibody (189-238 aa) (STJ91678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of various cells using ARHGAP18 Polyclonal Antibody

Anti-ARHGAP18 antibody (517-566 aa) (STJ91682)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-ARHGAP22 antibody (565-614 aa) (STJ91683)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-ARHGAP23 antibody (470-550 aa) (STJ91684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of L929 cells using ARHGAP25 Polyclonal Antibody (STJ22673) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ARHGAP25 antibody (1-370) (STJ22673)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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