Immunofluorescence Antibodies | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200

Anti-IL17C antibody (81-130 aa) (STJ98799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-tonsil, antibody was diluted at 1:100

Anti-IL17D antibody (141-190 aa) (STJ99011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-colon-cancer, antibody was diluted at 1:100

Anti-IL17F antibody (81-130 aa) (STJ99010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot with Anti-IL17RA Antibody (STJ501422) with peptide at 1:750 dilution in buffer. 12% gel.

Anti-IL17RA antibody (110-150) (STJ501422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-IL17RA antibody (110-150) {Biotin} (STJ501423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-IL17RA antibody (110-150) {FITC} (STJ501424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-IL18 antibody (1-193 aa) [SRM5148] (STJ11106987)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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STJ71925 (0. 1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-IL18 antibody (Internal) (STJ71925)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human

£312.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-IL18 antibody (STJ192030)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-IL18 antibody [PT0148R] (STJA0039712)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£0.00

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Anti-IL18 antibody [SRM13359] (STJ11108089)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£0.00

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Immunofluorescence analysis of A549 cells using IL18BP antibody (STJ28528). Blue: DAPI for nuclear staining.

Anti-IL18BP antibody (31-194) (STJ28528)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of NCI-H460 cells, using IL18R1/IL-18 R alpha antibody (STJ24172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL18R1 antibody (19-320) (STJ24172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200

Anti-IL18RAP antibody (291-340 aa) (STJ98755)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 293T Hela VEC KB mouse-kidney lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000

Anti-IL19 antibody (51-100 aa) (STJ98773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-IL1A antibody (8-57 aa) (STJ93686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of THP-1 cells using IL1 Beta Rabbit polyclonal antibody (STJ113778) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-IL1B antibody (1-269) (STJ113778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various lysates using IL1 Beta Rabbit pAb (STJ24174) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-IL1B antibody (1-269) (STJ24174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of THP-1 cells, using IL1 Beta antibody (STJ11101739) at 1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/mL) at 37 °C for 8 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-IL1B antibody (117-269) [S9MR] (STJ11101739)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, IL-1 Beta Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IL1B antibody (181-230 aa) (STJ96566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using IL-1 Beta monoclonal antibody against HEK293 (1) and IL-1 Beta-hIgGFc transfected HEK293 (2) cell lysate.

Anti-IL1B antibody [3A6] (STJ98171)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of recombinant mouse IL-1 beta protein, using IL1 Beta Rabbit monoclonal antibody (STJ11104780) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10ng/5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-IL1Beta antibody (118-269) [S4780RM] (STJ11104780)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse

£146.50 - £642.50

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Western blot analysis of mouse kidney, mouse heart, mouse brain, mouse spleen and rat spleen using IL1F10 antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-IL1F10 antibody (101-150 aa) (STJ97678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of MCF7 cells using IL-1RI Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL1R1 antibody (31-80 aa) (STJ96628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of PC12 cells using IL-1RII Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL1R2 antibody (141-190 aa) (STJ96835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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STJ72269 (0. 5µg/ml) staining of LNCaP (A) and (1ug/ml) MOLT-4 (B) cell-lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-IL1R2 antibody (Internal) (STJ72269)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human

£312.50

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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200

Anti-IL1RL1 antibody (251-300 aa) (STJ98813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using IL36R Rabbit polyclonal antibody (STJ112130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-IL1RL2 antibody (1-116) (STJ112130)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Confocal imaging of mouse tail ear using IL1RA Rabbit monoclonal antibody (STJ11101277, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-IL1RN antibody (100-177) [S7MR] (STJ11101277)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of A-431 cells, using IL1RA antibody (STJ24179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL1RN antibody (24-177) (STJ24179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using IL1RN Rabbit polyclonal antibody (STJ11104175) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IL1RN antibody (27-178) (STJ11104175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using IL-2 Polyclonal Antibody

Anti-IL2 antibody (16-65 aa) (STJ93688)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-IL2 antibody [SRM13481] (STJ11108211)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Mouse/Rat

£0.00

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Anti-IL2 antibody [SRM15021] (STJ11109751)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£0.00

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Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:200

Anti-IL20 antibody (81-130 aa) (STJ98782)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using IL-20R Alpha Polyclonal Antibody

Anti-IL20RA antibody (173-222 aa) (STJ93690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using IL-20R Beta Polyclonal Antibody

Anti-IL20RB antibody (141-190 aa) (STJ93691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using (STJ29315) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-IL21 antibody (25-162) (STJ29315)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-IL21 antibody [SRM13293] (STJ11108023)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human/Rat

£0.00

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Western blot analysis of extracts of normal 293T cells and 293T transfected with IL22 using IL22 Rabbit mAb (STJ11107003) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 45s.

Anti-IL22 antibody (34-179 aa) [SRM5159] (STJ11107003)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of HL-60, using IL22 antibody (STJ27972) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IL22 antibody (34-179) (STJ27972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of L929 cells using IL23R Rabbit polyclonal antibody (STJ24181) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-IL23R antibody (510-629) (STJ24181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293 cells using MDA-7 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IL24 antibody (131-180 aa) (STJ96776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with 17E/IL25 using IL25 Rabbit pAb (STJ110551) at 1:2000 dilution. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/> Lysates/ proteins: 25 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 90s.

Anti-IL25 antibody (33-177) (STJ110551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:200

Anti-IL27 antibody (1-50 aa) (STJ98795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using CD25 antibody (STJ24183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-IL2RA antibody (153-167) (STJ24183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells, using IL-2R alpha/CD25 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-IL2RA antibody (223-272 aa) (STJ93693)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, CD25 monoclonal antibody (Q22) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IL2RA antibody [Q22] (STJ97009)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£48.00 - £252.00

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