Immunofluorescence Antibodies | St John's Laboratory

Immunofluorescence analysis of human-kidney tissue. 1, HSP70 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-HSP70 antibody (STJ96789)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Immunofluorescence analysis of Hela cell. 1, AIM2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HSP70 monoclonal antibody (3G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HSP70 antibody [3G10] (STJ96944)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Anti-Hsp70 antibody [PT0218R] (STJA0039689)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

Choose Options

Western blot analysis of HSP70 at dilution of 1:1000

Anti-HSP70 antibody [SM0470] (STJA0010470)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

Choose Options

Western blot analysis of various cells using HSP70 Polyclonal Antibody diluted at 1：1000

Anti-HSP70L antibody (221-270 aa) (STJ93607)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Anti-Hsp90 antibody [PT0053R] (STJA0039749)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

Choose Options

Western blot analysis of HSP90. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-Hsp90 antibody [SM0475] (STJA0010475)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

Choose Options

Anti-Hsp90 Beta antibody [SRM14646] (STJ11109376)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Rice

£0.00

Add to Cart The item has been added

Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g Hsp90 Alpha antibody (STJ11102219). Western blot was performed from the immunoprecipitate using Hsp90 Alpha antibody (STJ11102219) at a dilution of 1:1000.

Anti-HSP90AA1 antibody (1-100) [S9MR] (STJ11102219)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

Choose Options

Western blot analysis of Jurkat cells using HSP 90 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-HSP90AA1 antibody (251-300 aa) (STJ96692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunofluorescence analysis of U2OS cells using Hsp90 Alpha Rabbit polyclonal antibody (STJ24087). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.

Anti-HSP90AA1 antibody (433-732) (STJ24087)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of various lysates using Hsp90 Alpha Rabbit pAb (STJ114322) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-HSP90AA1 antibody (629-732) (STJ114322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue with HSP90 a Mouse mAb diluted at 1:2, 000.

Anti-HSP90AA1 antibody (660-740 aa) [1F6] (STJ97496)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Immunofluorescence analysis of Hela cell. 1, HSP90A Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HSP90AA1 antibody (683-732 aa) (STJ93622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunofluorescence analysis of U2OS cells using Hsp90 Beta Rabbit polyclonal antibody (STJ11104548). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSP90AB1 antibody (1-300) (STJ11104548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of lysates from wild type (WT) and HSP90AB1 knockout (KO) HeLa cells, using [KO Validated] Hsp90 Beta Rabbit polyclonal antibody (STJ119988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HSP90AB1 antibody (1-300) (STJ119988)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£126.50 - £618.50

Choose Options

Western blot analysis of various cells using HSP90 Beta Polyclonal Antibody diluted at 1：2000

Anti-HSP90AB1 antibody (226-275 aa) (STJ93609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

Choose Options

Western blot analysis using HSP90 Beta monoclonal antibody against Jurkat (1) , A431 (2) , HeLa (3) , A549 (4) , HEK293 (5) , K562 (6) , NIH/3T3 (7) , PC-12 (8) and Cos7 (9) cell lysate.

Anti-HSP90AB1 antibody [5G4] (STJ98144)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£149.00 - £360.00

Choose Options

Immunofluorescence analysis of paraffin-embedded Mouse colon cancer tissue using Grp94 Rabbit polyclonal antibody (STJ24090) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-HSP90B1 antibody (40-310) (STJ24090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Immunofluorescence analysis of NIH-3T3 cells using Grp94 Polyclonal Antibody (STJ28194) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HSP90B1 antibody (501-750) (STJ28194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of various cells using Endoplasmin Polyclonal Antibody diluted at 1：1000

Anti-HSP90B1 antibody (754-803 aa) (STJ92925)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

Choose Options

Anti-HSPA12A PT® antibody [PT1108R] (STJA0039887)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

Choose Options

Western blot analysis of lysates from U-87MG cells, using HSPA13 Rabbit pAb (STJ116234) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-HSPA13 antibody (165-471) (STJ116234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Confocal imaging of NIH/3T3 cells using HSPA14 Rabbit monoclonal antibody (STJ11101806, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-HSPA14 antibody (1-100) [S6MR] (STJ11101806)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

Choose Options

Immunocytochemistry analysis of HspA14 (green) in Jurkat using HspA14 antibody, and DAPI (blue).

Anti-HSPA14 antibody (75-89 aa) [R02-1I2] (STJA0012419)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

£264.50

Choose Options

Anti-HspA14 PT® antibody [PT1115R] (STJA0039894)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

Choose Options

Immunofluorescence analysis of U2OS cells using HSP70 Rabbit polyclonal antibody (STJ114814) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSPA1A antibody (500-600) (STJ114814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of various lysates using HSPA2 Rabbit pAb (STJ110211) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-HSPA2 antibody (400-639) (STJ110211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Confocal imaging of paraffin-embedded Rat testis tissue using HSPA2 Rabbit monoclonal antibody (STJ11102321, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-HSPA2 antibody (488-637) [S1MR] (STJ11102321)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

Choose Options

Immunofluorescence analysis of A431 cells using HSPA4L Rabbit polyclonal antibody (STJ119695) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSPA4L antibody (500-600) (STJ119695)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of 3T3 Cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-HSPA5 antibody (505-570 aa) (STJ98969)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Fish

£49.00 - £261.00

Choose Options

Immunofluorescence analysis of U2OS cells using BiP/GRP78 Rabbit polyclonal antibody (STJ113776) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HSPA5 antibody (550-654) (STJ113776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of 1) Hela, 2) Rat LiverTissue with GRP78/Bip Mouse mAb diluted at 1:2, 000.

Anti-HSPA5 antibody [10C9] (STJ97523)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of extracts from 3T3, A549, C6 and Hela cell lysates using BiP/GRP78 mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:72KDa.

Anti-HSPA5 antibody [4F11-D5-C10] (STJ99068)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse/Rat/Hamster

£149.00 - £360.00

Choose Options

Anti-HSPA5 antibody [8G3] (STJ97526)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat

£49.00 - £261.00

Choose Options

STJ73249 (0. 1µg/ml) staining of HepG2 (A) , HeLa (B) and MCF7 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-HSPA5/GRP78 antibody (118-131) (STJ73249)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/WB

Reactivity :

Human

£312.50

Choose Options

STJ73250 (1µg/ml) staining of HepG2 (A) and MCF7 (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-HSPA5/GRP78 antibody (Internal) (STJ73250)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human

£312.50

Choose Options

Immunofluorescence analysis of L929 cells using Hsc70/HSPA8 Rabbit polyclonal antibody (STJ24099) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HSPA8 antibody (510-615) (STJ24099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£110.50 - £505.50

Choose Options

Western blot analysis of 1) Hela, 2) HepG2, 3) Raw, 4) Mouse Brain, 5) Rat Brain, 6) Rat Liver using HSC 70 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-HSPA8 antibody (588-638) (STJA0006703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

STJ70949 (0. 03µg/ml) staining of HepG2 (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-HSPA8-Isoform 1 antibody (Internal) (STJ70949)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/IHC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

Choose Options

Anti-HSPA9/Mortalin/GRP75 antibody [SAA0812] (STJA0019624)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

Choose Options

Immunofluorescence analysis of HeLa cells using HSP27/HSP27/HSPB1 antibody (STJ118760) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HSPB1 antibody (1-120) (STJ118760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

Confocal imaging of NIH/3T3 cells using HSP27/HSPB1 Rabbit monoclonal antibody (STJ11101283, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-HSPB1 antibody (100-205) [S3MR] (STJ11101283)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

Choose Options

Immunofluorescence analysis of mouse-liver tissue. 1, HSP27 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-HSPB1 antibody (48-97 aa) (STJ93617)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis using HSP27 monoclonal antibody against HeLa (1) , A549 (2) , Jurkat (3) , A431 (4) , HEK293 (5) , HepG2 (6) and PC-12 (7) cell lysate.

Anti-HSPB1 antibody [5D7] (STJ98141)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Rat

£149.00 - £360.00

Choose Options

Western blot analysis of Hela with HSP27 Mouse mAb diluted at 1:2, 000

Anti-HSPB1 antibody [Mix-mA] (STJ97485)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£49.00 - £261.00

Choose Options

Immunofluorescence analysis of L929 cells using HSPB11 Rabbit polyclonal antibody (STJ11100181) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSPB11 antibody (1-144) (STJ11100181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Immunofluorescence analysis of U2OS cells using HSPB2 antibody (STJ26207). Blue: DAPI for nuclear staining.

Anti-HSPB2 antibody (1-182) (STJ26207)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

Choose Options