Anti-GABRA4 antibody (149-199 aa) (STJ197387)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide derived from GABA A Receptor Alpha 4 at the amino acid range 149-199
General Information
| Short Description | Rabbit polyclonal anti-Gamma-aminobutyric acid receptor subunit alpha-4 receptor subunit alpha-4 (149-199 aa) for use in WB, IHC and IF in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000-2000IHC 1:100-200IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GABRA4 |
| Gene ID | 2557 |
| Uniprot ID | GBRA4_HUMAN |
| Immunogen | Synthetic peptide derived from GABA A Receptor Alpha 4 at the amino acid range 149-199 |
| Immunogen Region | 149-199 aa |
| Specificity | GABA A Receptor Alpha 4 protein (A226) detects endogenous levels of GABA A Receptor Alpha 4 |
Additional Info
| Function | Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. GABAARs containing alpha-4 are predominantly extrasynaptic, contributing to tonic inhibition in dentate granule cells and thalamic relay neurons. Extrasynaptic alpha-4-containing GABAARs control levels of excitability and network activity. GABAAR containing alpha-4-beta-3-delta subunits can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness. |
| Protein Name | Gamma-Aminobutyric Acid Receptor Subunit Alpha-4Gaba(A Receptor Subunit Alpha-4Gabaar Subunit Alpha-4 |
| Database Links | Reactome: R-HSA-977443 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinPostsynaptic Cell Membrane |
| Alternative Antibody Names | Anti-Gamma-Aminobutyric Acid Receptor Subunit Alpha-4 antibodyAnti-Gaba(A Receptor Subunit Alpha-4 antibodyAnti-Gabaar Subunit Alpha-4 antibodyAnti-GABRA4 antibody |
Information sourced from Uniprot.org