Immunocytochemistry Antibodies | St John's Laboratory

Anti-Phospho-Histone H2A.X-Ser139 antibody [R03-2Z1] (STJA0036230)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various lysates using Phospho-Histone H3-S10 Rabbit pAb (STJ11100981) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H3-S10 antibody (STJ11100981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Anti-Phospho-Histone H3-S10 antibody [SRM13680] (STJ11108410)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£0.00

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Western blot analysis of various lysates using Phospho-Histone H3-S10/T11 Rabbit pAb (STJ11101033) at 1:1000 dilution. Both NIH/3T3 cells and Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour and treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H3-S10/T11 antibody (STJ11101033)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-Histone H3-S28 Rabbit pAb (STJ11100980) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-Histone H3-S28 antibody (STJ11100980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, Histone H3 (phospho Ser10) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-Histone H3-Ser10 antibody (1-50 aa) (STJ90289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IHC-F/FC/ICC/IF/ELISA

Reactivity :

Human/Mouse/Rat/D.melanogaster

£49.00 - £261.00

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Anti-Phospho-Histone H3-Ser10 antibody [R06-6O9] (STJA0035817)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-Histone H3-Ser10/Thr11 antibody [R08-6C4] (STJA0035070)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-Histone H3-Ser28 antibody [R03-7K5] (STJA0034415)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Western blot analysis of lysates from C6 cells using Phospho-Histone H3-T11 Rabbit pAb (STJ22169) at 1:2000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-Histone H3-T11 antibody (STJ22169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of various lysates using Phospho-Histone H3-T45 Rabbit pAb (STJ11101034) at 1:1000 dilution. Hela cells, NIH/3T3 cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-Histone H3-T45 antibody (STJ11101034)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of lysates from C2C12 cells, using Phospho-Histone H3-T6 Rabbit pAb (STJ11101035) at 1:400 dilution. C2C12 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-Histone H3-T6 antibody (STJ11101035)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Anti-Phospho-Histone H3-Thr3 antibody [R08-9D6] (STJA0033904)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-Histone H3-Thr3 antibody [R21-3K-2] (STJA0035656)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-HSF1-Ser326 antibody [R08-4I1] (STJA0034439)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

£140.50 - £308.50

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Anti-Phospho-HSF1-Ser326 antibody [R77-7H-8] (STJA0035659)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-Hsp27-Ser78 antibody [R01-8F1] (STJA0036207)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-HSP27/HSPB1-S15 antibody [SRM13690] (STJ11108420)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£0.00

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Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S78 Rabbit pAb (STJ22181) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes or treated by serum-starvation overnight or treated by Anisomycin (25 Mu g/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-HSPB1-S78 antibody (STJ22181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Anti-Phospho-iNOS-Tyr-1055 antibody (STJA0041300)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Phospho-Integrin Beta 4-Tyr-1494 antibody (STJA0041255)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Phospho-Integrin Beta 4-Tyr-1526 antibody (STJA0041256)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Phospho-IRF3-S396 antibody [SRM13691] (STJ11108421)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£0.00

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Anti-Phospho-IRF3-Ser386 antibody [R05-2Q2] (STJA0036233)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-ITGB4-Tyr-1494 antibody (STJA0005452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Anti-Phospho-ITGB4-Tyr-1526 antibody (STJA0005453)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot analysis of lysates from RAW264. 7 cells, using Phospho-Jak1-Y1034/Y1035 Rabbit pAb (STJ110866) at 1:1000 dilution. RAW264. 7 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-JAK1-Y1022/1023 antibody (STJ110866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Anti-Phospho-JAK2-Tyr1007/1008 antibody [R02-1C5] (STJA0036229)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-JNK-Thr-183/Tyr-185 antibody [M268] (STJA0041259)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat

£542.50

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Anti-Phospho-JNK-Thr183 antibody [R04-9D2] (STJA0035957)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Confocal imaging of paraffin-embedded Rat brain tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit monoclonal antibody (STJ11105826, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-Phospho-JNK1 + JNK2 + JNK3-T183/Y185-T183/Y185-T221/Y223 antibody [S5826RM] (STJ11105826)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-c-Jun-S63 Rabbit mAb (STJ11102553) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-JUN-S63 antibody [S3MR] (STJ11102553)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-Phospho-JUP-Tyr-550 antibody (STJA0005309)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Immunostaining of Chinese Hamster Overy (CHO) cells stably transfected with KV3. 1b gene with the phospho-Ser503 Kv3. 1 subunit antibody (STJA0003752, green, 1:400). The image on the right shows cells that have been treated with the protein kinase C activator PMA (500nM) while the control cells are on the left.

Anti-Phospho-KCNC1-Ser503 antibody (STJA0003752)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC

Reactivity :

Hamster/Mouse/Rat

£488.50

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Anti-Phospho-KDR-Tyr-801 antibody (STJA0005641)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot with Anti-Phospho-KIRREL Antibody (STJ502567) with 1) NEPH1 peptide. Lane 2) STJ502567 with NEPH1 peptide. Lane 3) STJ502567 with PNEPH1 peptide. Antibody at 1:500 dilution in buffer.

Anti-Phospho-KIRREL-p737/738 antibody (STJ502567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Chicken/Human/Mouse/Rat/Zebra Fish

£497.50

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Anti-Phospho-KIRREL-p737/738 antibody {Biotin} (STJ502568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Chicken/Human/Mouse/Rat/Zebra Fish

£551.50

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Anti-Phospho-KIRREL-p737/738 antibody {FITC} (STJ502569)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Chicken/Human/Mouse/Rat/Zebra Fish

£551.50

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Anti-Phospho-LAT-Tyr191 antibody [R06-3H7] (STJA0035752)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-LATS1-T1079+LATS2-T1041-T1079+T1041 antibody [SRM15143] (STJ11109873)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-LCK-Y394 Rabbit pAb (STJ22226) at 1:1000 dilution. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-LCK-Y394 antibody (STJ22226)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-LIMK1-T508 Rabbit pAb (STJ110833).

Anti-Phospho-LIMK1-T508 antibody (STJ110833)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-LIMK2-T505 Rabbit pAb (STJ22229). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-LIMK2-T505 antibody (STJ22229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-LRRK2-Ser935 antibody [R94-3M-6] (STJA0035658)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Anti-Phospho-Lyn-Tyr396 antibody [R07-3H2] (STJA0036232)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human/Mouse

£133.50 - £281.50

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Western blot analysis of lysates from HepG2 cells using Phospho-MEK2-T394 Rabbit pAb (STJ22237). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MAP2K2-T394 antibody (STJ22237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human

£126.50 - £618.50

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Anti-Phospho-MAP2K4-S257 antibody [SRM13701] (STJ11108431)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from HL60 cells, using Phospho-MEK6-S207 Rabbit pAb (STJ22247). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MAP2K6-S207 antibody (STJ22247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Rat

£126.50 - £618.50

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