• Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-T6 antibody (STJ11101035) at 1:1000 dilution. Hela cells and NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. HeLa cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-T6 Rabbit polyclonal antibody (STJ11101035) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human colon using Phospho-Histone H3-T6 antibody (STJ11101035) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-T6 Rabbit polyclonal antibody (STJ11101035) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat brain using Phospho-Histone H3-T6 antibody (STJ11101035) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of C6 cells using Phospho-Histone H3-T6 Rabbit polyclonal antibody (STJ11101035) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone H3-T6 antibody (STJ11101035) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-Histone H3-T6 in paraffin-embedded mouse pancreas using Phospho-Histone H3-T6 Rabbit polyclonal antibody (STJ11101035) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse pancreas using Phospho-Histone H3-T6 antibody (STJ11101035) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-Histone H3-T6 in paraffin-embedded mouse testis using Phospho-Histone H3-T6 Rabbit polyclonal antibody (STJ11101035) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using Phospho-Histone H3-T6 rabbit polyclonal antibody (STJ11101035) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Phospho-Histone H3-T6 in paraffin-embedded rat brain using Phospho-Histone H3-T6 Rabbit polyclonal antibody (STJ11101035) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-T6 rabbit polyclonal antibody (STJ11101035) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Phospho-Histone H3-T6 in paraffin-embedded human colon using Phospho-Histone H3-T6 Rabbit polyclonal antibody (STJ11101035) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-T6 rabbit polyclonal antibody (STJ11101035) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from C2C12 cells, using Phospho-Histone H3-T6 Rabbit polyclonal antibody (STJ11101035) at 1:400 dilution. C2C12 cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-Histone H3-T6 antibody (STJ11101035)

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STJ11101035

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Histone H3-T6 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around T6 of human (NP_003520.1).
Immunogen Sequence: KQTAR
Background Predicted to enable metal ion binding activity. Acts upstream of or within intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator and tumor necrosis factor-mediated signaling pathway. Located in cytoplasmic side of late endosome membrane; cytoplasmic side of lysosomal membrane; and nucleus.

Information sourced from Uniprot.org

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