Anti-Phospho-MAP2K4-S257 antibody [SRM13701] (STJ11108431)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal Phospho-Dual Specificity Mitogen-Activated Protein Kinase Kinase 4-S257 antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM13701 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB, 1:1000-1:5000IHC-P, 1:50-1:200IF/ICC, 1:50-1:200IP, 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your |
| Formulation | PBS containing 50% Glycerol and 0.05% BSA, preserved with proclin300 or Sodium Azide (as specified on the Certificate of Analysis) , pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAP2K4 |
| Gene ID | 6416 |
| Uniprot ID | MP2K4_HUMAN |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Immunogen Sequence | Human |
Additional Info
| Post Translational Modifications | Activated by phosphorylation on Ser-257 and Thr-261 by MAP kinase kinase kinases (MAP3Ks). |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to pro-inflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. |
| Protein Name | Dual Specificity Mitogen-Activated Protein Kinase Kinase 4Map Kinase Kinase 4Mapkk 4Jnk-Activating Kinase 1Mapk/Erk Kinase 4Mek 4Sapk/Erk Kinase 1Sek1Stress-Activated Protein Kinase Kinase 1Sapk Kinase 1Sapkk-1Sapkk1C-Jun N-Terminal Kinase Kinase 1Jnkk |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-2871796Reactome: R-HSA-450321Reactome: R-HSA-5210891Reactome: R-HSA-5684264 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 4 antibodyAnti-Map Kinase Kinase 4 antibodyAnti-Mapkk 4 antibodyAnti-Jnk-Activating Kinase 1 antibodyAnti-Mapk/Erk Kinase 4 antibodyAnti-Mek 4 antibodyAnti-Sapk/Erk Kinase 1 antibodyAnti-Sek1 antibodyAnti-Stress-Activated Protein Kinase Kinase 1 antibodyAnti-Sapk Kinase 1 antibodyAnti-Sapkk-1 antibodyAnti-Sapkk1 antibodyAnti-C-Jun N-Terminal Kinase Kinase 1 antibodyAnti-Jnkk antibodyAnti-MAP2K4 antibodyAnti-JNKK1 antibodyAnti-MEK4 antibodyAnti-MKK4 antibodyAnti-PRKMK4 antibodyAnti-SEK1 antibodyAnti-SERK1 antibodyAnti-SKK1 antibody |
Information sourced from Uniprot.org