Primary Antibodies | St John's Laboratory

Immunohistochemistry analysis of WIPF1 in paraffin-embedded mouse spleen using WIPF1 Rabbit polyclonal antibody (STJ11102826) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-WIPF1 antibody (400-500) (STJ11102826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Western blot analysis of extracts of various cell lines, using CD73/NT5E rabbit polyclonal antibody (STJ11102804) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NT5E antibody (32-264) (STJ11102804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Immunohistochemistry analysis of MAP3K4 in paraffin-embedded human placenta using MAP3K4 Rabbit polyclonal antibody (STJ11102801) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAP3K4 antibody (1-100) (STJ11102801)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

$151.88 - $708.08

Choose Options

Western blot analysis of NIH/3T3, using ARRB2 antibody (STJ11102772) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ARRB2 antibody (300-400) (STJ11102772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Immunoprecipitation analysis of 300 Mu g extracts from HepG2 cells using 3 Mu g ARPC2 antibody (STJ11102755). Western blot was performed from the immunoprecipitate using ARPC2 antibody (STJ11102755) at a dilution of 1:1000.

Anti-ARPC2 antibody (200-300) [S5MR] (STJ11102755)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

Choose Options

Immunohistochemistry analysis of paraffin-embedded human liver using CD90/Thy1 Rabbit monoclonal antibody (STJ11102747) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD90/Thy1 antibody (1-100) [S7MR] (STJ11102747)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

$174.83 - $775.58

Choose Options

Immunohistochemistry analysis of S100A8 in paraffin-embedded rat spleen tissue using S100A8 Rabbit monoclonal antibody (STJ11102740) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-S100A8 antibody (1-93) [S0MR] (STJ11102740)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

Choose Options

Immunohistochemistry analysis of paraffin-embedded mouse spleen using JAK1 Rabbit monoclonal antibody (STJ11102738) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-JAK1 antibody (600-700) [S8MR] (STJ11102738)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

Choose Options

Immunofluorescence analysis of U-937 cells using TNFRSF17 Rabbit polyclonal antibody (STJ11102730) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-TNFRSF17 antibody (1-100) (STJ11102730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Immunoprecipitation analysis of 300 Mu g extracts of THP-1 cells using 3 Mu g NCF4/p40-phox antibody (STJ11102723). Western blot was performed from the immunoprecipitate using NCF4/p40-phox antibody (STJ11102723) at a dilution of 1:1000.

Anti-NCF4/p40-phox antibody (240-339) [S3MR] (STJ11102723)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

Choose Options

Immunoprecipitation analysis of 300 Mu g extracts from HepG2 cells using 3 Mu g Collagen III alpha 1/COL3A1 Rabbit monoclonal antibody (STJ11102715). Western blot was performed from the immunoprecipitate using Collagen III alpha 1/COL3A1 Rabbit monoclonal antibody (STJ11102715) at a dilution of 1:1000.

Anti-Collagen III alpha 1/COL3A1 antibody (150-400) [S5MR] (STJ11102715)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

Choose Options

Western blot analysis of A549, using BRMS1 rabbit monoclonal antibody (STJ11102709) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BRMS1 antibody (1-100) [S9MR] (STJ11102709)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

Choose Options

Immunofluorescence analysis of U2OS cells using ACAA2 Rabbit monoclonal antibody (STJ11102704) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ACAA2 antibody (298-397) [S4MR] (STJ11102704)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

Choose Options

Western blot analysis of various lysates, using HDGF antibody (STJ11102694) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HDGF antibody (100-200) [S4MR] (STJ11102694)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

Choose Options

Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g PGD antibody (STJ11102692). Western blot was performed from the immunoprecipitate using PGD antibody (STJ11102692) at a dilution of 1:1000.

Anti-PGD antibody (384-483) [S2MR] (STJ11102692)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

Choose Options

Immunohistochemistry analysis of paraffin-embedded mouse brain using TSC2 Rabbit polyclonal antibody (STJ11102686) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-TSC2 antibody (1160-1255) (STJ11102686)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Immunofluorescence analysis of C6 cells using DEK Rabbit monoclonal antibody (STJ11102678) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DEK antibody (51-200) [S8MR] (STJ11102678)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Rat

$174.83 - $775.58

Choose Options

Immunofluorescence analysis of U-2 OS cells using KIFC1 Rabbit monoclonal antibody (STJ11102673) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-KIFC1 antibody (1-100) [S3MR] (STJ11102673)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

Choose Options

STJ73762 (1. 5µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ACE2 antibody (N-Term) (STJ73762)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/FC

Reactivity :

Human

$421.88

Choose Options

STJ73756 (0. 1µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CTNNB1/catenin beta-1 antibody (Internal) (STJ73756)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

$421.88

Choose Options

STJ73754 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Golgi/ER and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-TLR4/CD284 antibody (Internal) (STJ73754)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/IHC/FC

Reactivity :

Human/Mouse/Rat

$421.88

Choose Options

STJ73734 (0. 1µg/ml) staining of A549 (A) and Daudi (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-COX2/PTGS2 antibody (C-Term) (STJ73734)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Dog/Pig/Cow

$421.88

Choose Options

Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0. 01M containing Triton X 0. 005% for 15 minutes. Cells were washed and incubated with 100 µl of Rabbit antibody to TGN (STJ13100506) diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 µl of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.

Anti-TGN38 antibody (STJ13100506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse/Human/Marmoset

$447.53

Choose Options

Confocal microscopy on isolated monocytes. MPO detected with Rabbit antibody to MPO: red; Hoechst: blue; MHC class II: green. Magnification: 200 X

Anti-MPO antibody (STJ13100487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

$447.53

Choose Options

Anti-mScarlet antibody at 1/2, 500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution

Anti-mScarlet antibody (STJ140286)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

RFP

$182.93 - $532.58

Choose Options

Immunofluorescence in Drosophila larvae NMJ syt1-GFP (syt1 is a synaptic protein) muscle 6/7 using anti-GFP conjugated to DyLight®550 at 1/500

Anti-GFP antibody {DyLight®550} (STJ140276)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IF

Reactivity :

GFP

$191.03 - $575.78

Choose Options

Anti-mEmerald antibody at 1/2, 500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-mEmerald antibody (STJ140226)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

$182.93 - $532.58

Choose Options

Anti-Asense antibody at 1:1,000 dilution; protein at 40 ng per lane; chicken polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-Asense antibody (254 aa C-Term) (STJ140181)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

D.melanogaster

$182.93 - $532.58

Choose Options

Anti-BDNF antibody [3B2] (STJA0002906)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF

Reactivity :

Human/Mouse/Rat/Guinea Pig

$591.98 - $3,378.38

Choose Options

Anti-Papillomavirus type 1 E2-protein antibody (199-208 aa) [5E11] (STJA0002816)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF/IP

Reactivity :

PV

$591.98 - $3,378.38

Choose Options

Anti-CDNF antibody (126-187 aa) [6G5] (STJA0002808)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF/IHC

Reactivity :

Human

$591.98 - $3,378.38

Choose Options

Anti-CD15/FUT4/LeX antibody [FR4A5] (STJ16100998)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human

$415.13 - $1,956.83

Choose Options

Anti-NCAM antibody [123C3] (STJ16100875)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/IHC-F/IF

Reactivity :

Human

$617.63

Choose Options

Anti-CD63 antibody [MEM-259] (STJ16100870)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/IHC-P/ICC

Reactivity :

Human

$582.53

Choose Options

Anti-Alpha-Tubulin antibody [TU-01] (STJ16100473)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ICC/IP/FC

Reactivity :

Mouse/Pig/Human/Turkey/Eisenia/Paramecium/Nicotiana/Yeast/Arabidopsis

$655.43

Choose Options

Anti-CD1a antibody [EP3622] (STJ16100442)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$1,110.38 - $2,160.68

Choose Options

Anti-Melanoma-Associated Antigen antibody [KBA.62] (STJ16100389)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$1,172.48 - $1,603.13

Choose Options

Anti-Keratin 18 antibody [DA-7] (STJ16100260)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IP/WB/IHC-P/ICC/ELISA

Reactivity :

Human

$547.43

Choose Options

Anti-Desmin antibody [D33] (STJ16100230)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/IHC-F

Reactivity :

Human

$369.23

Choose Options

STJ73733 (1µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Cathepsin B antibody (Internal) (STJ73733)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/IHC/FC

Reactivity :

Human

$421.88

Choose Options

STJ73669 (8µg/ml) staining of paraffin embedded Human Prostate. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-Plasminogen Activator/PLAU antibody (C-Term) (STJ73669)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human

$421.88

Choose Options

Chen, Kuikui, et al. "Investigation of the lipid-lowering mechanisms and active ingredients of Danhe granule on hyperlipidemia based on systems pharmacology. " Frontiers in pharmacology 11 (2020) : 528.

Anti-ABCA1 antibody (1112-1180 aa) (STJ192951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Hamster

$64.80 - $340.20

Choose Options

Human placental mesenchymal stromal cells modulate IFN-γ and IL-10 secretion by CD4+T cells via CD73, and alleviate intestinal damage in mice with graft-versus-host disease. INTERNATIONAL IMMUNOPHARMACOLOGY Xiying Luan WB Human, mouse intestine, colon NCM460 cell, Caco-2 cell

Anti-TJP1 antibody (1600-1700 aa) (STJ191514)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Pig

$64.80 - $340.20

Choose Options

Immunofluorescence analysis of U2OS cells using GOLPH2 Rabbit polyclonal antibody (STJ29422) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GOLM1 antibody (112-401) (STJ29422)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Immunofluorescence analysis of paraffin-embedded mouse liver using Complement C3 Rabbit polyclonal antibody (STJ115248) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-C3a antibody (1364-1663) (STJ115248)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

Choose Options

Western blot analysis of various lysates using Beta-Actin Rabbit mAb (STJ11100960) at 1:50000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-ACTB antibody [S0MR] (STJ11100960)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Chicken/Zebrafish/Pig

$182.93 - $336.83

Choose Options

STJ73639 (0. 5µg/ml) staining of HEK293 (A) , HeLa (B) and (1ug/ml) NIH3T3 (C) Nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-EDF1/MBF1 antibody (N-Term) (STJ73639)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC/IHC

Reactivity :

Human/Mouse/Rat

$421.88

Choose Options

STJ73509 (1µg/ml) staining of HEK293 nuclear cell lysate. (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Hdac2-Mouse antibody (Internal) (STJ73509)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

$421.88

Choose Options