ELISA Kits

Matched Antibody Pairs

A matched antibody pair is a capture and detection antibody combination selected to bind distinct, non-overlapping epitopes on the same target antigen, so both antibodies can bind the antigen at the same time without interference.1 Our range covers over 600 matched pairs across cytokines, chemokines, tumour biomarkers and extracellular matrix proteins, each supplied as a validated capture and detection antibody set for sandwich ELISA.

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How They Work Featured Pairs Research Targets Species Availability Building a Sandwich ELISA Support
How It Works

Capture and Detection Antibody Function

Sandwich ELISA requires two antibodies raised against the same antigen but specific for different regions of it.1 One antibody captures the antigen from the sample, and the second detects it once bound, producing a measurable signal only when both antibodies find their target.1 Antibody pairs supplied as a set have already been tested together to confirm that binding of one antibody does not block the other, which removes a major source of assay development delay for a researcher pairing individual antibodies from scratch.

1

Capture Antibody

Immobilised on the surface of the assay plate. It binds and concentrates the target antigen out of a complex sample such as serum, plasma or cell lysate, holding it in place for the steps that follow.

2

Detection Antibody

Added after the capture step. It binds a second, non-overlapping epitope on the same antigen, so it can still reach its target even while the capture antibody remains bound, and it carries or recruits the label that generates the final signal.

✓ Epitope compatibility already testedCapture and detection antibodies in a pair have been checked for simultaneous binding, removing the epitope-mapping step a researcher would otherwise run against a panel of candidate antibodies.
✓ Faster assay set-upCoating concentration and detection antibody titre still need optimising for a given sample type, but the underlying antibody combination does not need re-screening.
✓ Consistent lot-to-lot performanceCapture and detection components are manufactured and supplied together, so the pairing used for validation is the same pairing shipped with each order.
Browse By Research Area

Matched Antibody Pairs by Research Area

Our range covers targets across inflammation and immunology, oncology, neuroscience and extracellular matrix biology. Each table below lists a target together with its reactivity, typical application and research relevance, expand a target to see the specific antibody pairs available for it.

Inflammation & Immunity

Cytokines, chemokines and acute-phase proteins used to profile immune activation and inflammatory signalling by sandwich ELISA.

TargetReactivityApplicationNotesPairs
IFN-γHuman/MouseELISATh1 cytokine central to macrophage activation and antiviral and antitumour immune responses
IL-6HumanELISAPleiotropic cytokine driving acute-phase responses, haematopoiesis and immune regulation2
IL-6RHuman/RatELISAIL-6 receptor alpha subunit, required for classic (membrane-bound) IL-6 signalling
TNF-αHuman/MouseELISAPro-inflammatory cytokine central to immune activation and apoptosis signalling
CRPHuman/RatELISAAcute-phase protein produced by the liver, used as a marker of inflammation and infection
IFN-βHumanELISAType I interferon involved in antiviral immunity and studied in autoimmune disease research
CXCL2HumanELISANeutrophil-recruiting chemokine (Gro-Beta) studied in acute inflammation
GzmBHumanELISASerine protease released by cytotoxic T cells and NK cells during target cell killing

Oncology

Established tumour biomarkers used in cancer research and diagnostics development.

TargetReactivityApplicationNotesPairs
CA125HumanELISAMucin-16, an established biomarker in ovarian cancer research and diagnostics
CEAHumanELISACarcinoembryonic antigen, a marker used mainly in colorectal cancer research and monitoring
AFPHumanELISAAlpha-fetoprotein, a marker used in liver cancer and germ cell tumour research

Neuroscience

Targets tied to amyloid processing and amyloid precursor protein biology in neurodegeneration research.

TargetReactivityApplicationNotesPairs
Aβ 1-40HumanELISAAmyloid beta fragment central to Alzheimer's disease pathology research
ADAM10HumanELISAMetalloproteinase involved in amyloid precursor protein processing and neurodegeneration research

Extracellular Matrix & Tissue Remodelling

Proteases involved in cartilage and connective tissue turnover, relevant to arthritis and fibrosis research.

TargetReactivityApplicationNotesPairs
ADAMTS4HumanELISAAggrecanase implicated in cartilage degradation and arthritis research
ADAMTS5HumanELISARelated aggrecanase studied in connective tissue disorders
HTRA1HumanELISASerine protease involved in extracellular matrix degradation and fibrosis research
Reactivity

Antibody Pairs by Species

Reactivity, the species the antigen itself belongs to, is what determines whether a pair suits your model organism. Each tab below highlights key pairs available for that reactivity.

Assay Development

Building a Sandwich ELISA

A matched pair supplies the capture and detection antibodies, but coating concentration, blocking conditions and detection reagents still benefit from optimisation for your specific sample type.1 The five steps below outline the standard sandwich ELISA workflow.

Five steps to building a sandwich ELISA: capture antibody, blocking buffer, antigen, detection antibody with enzyme, signal
1
Coat & Capture
The capture antibody is coated onto the plate surface at the recommended concentration and left to bind overnight.
2
Block
A protein blocker such as BSA or casein is applied to reduce non-specific binding across the rest of the plate surface.
3
Sample & Bind
Sample or standard is added. Target antigen present in the sample binds the immobilised capture antibody.
4
Detect
The detection antibody is added and binds a second, non-overlapping epitope on the captured antigen.1
5
Read
An enzyme-substrate reaction develops a colour or chemiluminescent signal, read against a standard curve to quantify the analyte.
Learning & Support

Learning and Support

Optimising a sandwich ELISA?

Read our step-by-step guide: Direct ELISA Protocol, or see how to build and read a standard curve from serial dilutions.

Not sure which reactivity or target fits your assay? Talk to us and our team can help you select the right pair.

Can't Find Your Target?

Browse the full range of over 600 matched antibody pairs, or contact us if your target isn't listed and we'll help source or develop the right pair.

Browse Antibody Pairs

1. Lequin RM. Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA). Clin Chem. 2005;51(12):2415-2418.

2. Tanaka T, Narazaki M, Kishimoto T. IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol. 2014;6(10):a016295.

For Research Use Only. All products are intended for research purposes and are not for use in diagnostic or other medical procedures.

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