Vesicle-Mediated Transport | Research Area | St John's Laboratory

Western blot analysis of various lysates using CLTB Rabbit pAb (STJ110702) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-CLTB antibody (1-211) (STJ110702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CLTB antibody (168-218 aa) (STJ195831)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of HeLa cells using Clathrin heavy chain Rabbit polyclonal antibody (STJ114297) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CLTC antibody (1451-1675) (STJ114297)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various lysates using Clathrin heavy chain Rabbit pAb (STJ110196) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CLTC antibody (1550-1650) (STJ110196)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-CLTC antibody (482-532 aa) (STJ192903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Endogenous CLTC detected with at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-CLTC-H Chain antibody (50aa to N-Term) (STJ140022)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

€126.88 - €493.13

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CLTCL1 antibody (893-943 aa) (STJ195282)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CLVS1 antibody (16-66 aa) (STJ193509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CLVS2 antibody (26-76 aa) (STJ194403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various lysates using CLVS2 Rabbit polyclonal antibody (STJ119850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CLVS2 antibody (265-327) (STJ119850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€138.13 - €631.88

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Western blot with Anti-CNIH1 Antibody (STJ500603) and peptide on rat at 1:250 dilution in buffer for 1 hour at room temperature, from a 14% SDS gel.

Anti-CNIH1 antibody (90-120) (STJ500603)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-CNIH1 antibody (90-120) {Biotin} (STJ500604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-CNIH1 antibody (90-120) {FITC} (STJ500605)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CNIH2 antibody (10-60 aa) (STJ195561)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-CNIH3 antibody (44-94 aa) (STJ197989)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemistry analysis of COG1 in paraffin-embedded Human colon using COG1 Rabbit polyclonal antibody (STJ11102919) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-COG1 antibody (835-935) (STJ11102919)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-COG1 antibody (STJA0009446)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€61.25 - €326.25

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using COG2 antibody (STJ28007) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-COG2 antibody (1-280) (STJ28007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various lysates using COG3 Rabbit polyclonal antibody (STJ119817) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-COG3 antibody (1-300) (STJ119817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-COG3 antibody (STJA0007238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€61.25 - €326.25

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Western blot analysis of various lysates using COG4 Rabbit polyclonal antibody (STJ118551) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-COG4 antibody (310-410) (STJ118551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€138.13 - €631.88

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Western blot analysis of extracts of various cell lines, using COG6 antibody (STJ112357) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-COG6 antibody (508-657) (STJ112357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-COG6 antibody (STJA0009405)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-COG7 antibody (245-295 aa) (STJ193792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of U2OS cells using COG7 antibody (STJ11105702) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-COG7 antibody (511-770) (STJ11105702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

€138.13 - €631.88

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Anti-COG8 antibody (STJA0008221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€61.25 - €326.25

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Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A1 Rabbit polyclonal antibody (STJ119220) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COL1A1 antibody (1100-1200) (STJ119220)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various lysates using Collagen I/COL1A1 rabbit polyclonal antibody (STJ111079) at 1:4000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-COL1A1 antibody (1189-1205) (STJ111079)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Immunocytochemistry analysis of Collagen I alpha 1 (green) in Hela using Collagen I alpha 1 antibody, and DAPI (blue).

Anti-COL1A1 antibody (1208-1453 aa) [R02-5E5] (STJA0013087)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

€330.63

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Immunofluorescence analysis of NIH/3T3 cells using COL1A1 Rabbit polyclonal antibody (STJ11105651) at a dilution of 1:250 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COL1A1 antibody (1359-1464) (STJ11105651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Immunofluorescence analysis of paraffin-embedded human colon using Human Pro-Collagen I alpha Rabbit polyclonal antibody (STJ11105505) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-COL1A1 antibody (26-161) (STJ11105505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human

€138.13 - €631.88

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Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, Collagen I Mouse monoclonal antibody (4H10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-COL1A1 antibody (STJ98915)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-COL1A1 nanobody [SAA1295] (STJN000085)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

€473.13 - €2,040.63

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Immunofluorescence analysis of human-stomach tissue. 1, COL1A2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-COL1A2 antibody (1-50 aa) (STJ92383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various lysates using Collagen I/COL1A2 Rabbit polyclonal antibody (STJ119111) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-COL1A2 antibody (1161-1260) (STJ119111)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various cells using COL1A2 Polyclonal Antibody diluted at 1：1000

Anti-COL1A2 antibody (471-520 aa) (STJ92384)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A2 Rabbit polyclonal antibody (STJ28351) at dilution of 1:25. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COL1A2 antibody (501-600) (STJ28351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-COL1A2 antibody (STJ99633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Human colon tissue was stained with Anti-Collagen Type III (ABT-CIII) Antibody

Anti-COL3A1 antibody (100-200 aa) [ABT-CIII] (STJ196885)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

€0.00

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Immunohistochemical analysis of paraffin-embedded Skin. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-COL3A1 antibody (100-200 aa) [PT0118] (STJ196955)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

€0.00

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Immunofluorescence analysis of NIH/3T3 cells using Collagen III alpha 1/COL3A1 Rabbit polyclonal antibody (STJ23192) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COL3A1 antibody (1201-1301) (STJ23192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Immunocytochemistry analysis of Collagen III (green) in Hela using Collagen III antibody, and DAPI (blue)

Anti-COL3A1 antibody (201-400 aa) [R06-4E1] (STJA0012676)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

€330.63

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Immunofluorescence analysis of HeLa cells, using Collagen III Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL3A1 antibody (81-130 aa) (STJ92388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from 1) MCF7, 2) Hela, 3) 293T cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (cat: (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-COL3A1 antibody [Q76] (STJ97025)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of mouse-musle cells using COL4A1 Polyclonal Antibody diluted at 1：500

Anti-COL4A1 antibody (11-60 aa) (STJ92389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of Hela MCF-7 U2OS 293T KB 293T VEC cells using Collagen IV Polyclonal Antibody diluted at 1:800. Secondary antibody was diluted at 1:20000

Anti-COL4A1 antibody (1428-1443 aa) (STJ98599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of extracts of U-87 MG cells, using COL4A1 Antibody (STJ112627) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-COL4A1 antibody (1445-1669) (STJ112627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€138.13 - €631.88

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Western blot analysis of HeLa, using COL4A2 Rabbit polyclonal antibody (STJ29971) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-COL4A2 antibody (1486-1712) (STJ29971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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