![Anti-CD4 nanobody [SAA1296] (STJN000083)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/200696/412878/STJN000083-SDS-PAGE-1__21574.1749232421.jpg?c=1 "Anti-CD4 nanobody [SAA1296] (STJN000083)")
![Anti-CD4 nanobody [SAA1296] (STJN000083)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/200696/412878/STJN000083-SDS-PAGE-1__21574.1749232421.jpg?c=1 "Anti-CD4 nanobody [SAA1296] (STJN000083)")
Anti-CD4 nanobody [SAA1296] (STJN000083)
SPECIFICATIONS
ClonalityMonoclonal
HostAlpaca
ConjugationUnconjugated
IsotypeVHH-8His-Cys-tag
General Information
| Short Description | Alpaca monoclonal nanobody anti-T-Cell Surface Glycoprotein Cd4 is suitable for use in ELISA, Flow Cytometry and SPR research applications. |
| Applications | ELISA/FC/SPR |
| Host | Alpaca |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SAA1296 |
| Isotype | VHH-8His-Cys-tag |
| Conjugation | Unconjugated |
| Concentration | 1.07 mg/mL |
| Purification | Purified by Nickel column. |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at-20°C 12 months. Store at-80°C long term. |
Target Information
| Gene Symbol | CD4 |
| Gene ID | 920 |
| Uniprot ID | CD4_HUMAN |
Additional Info
| Post Translational Modifications | Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Phosphorylated by PKC.phosphorylation at Ser-433 plays an important role for CD4 internalization. |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1). Down-regulated by HIV-1 Vpu. Acts as a receptor for Human Herpes virus 7/HHV-7. |
| Protein Name | T-Cell Surface Glycoprotein Cd4T-Cell Surface Antigen T4/Leu-3Cd Antigen Cd4 |
| Database Links | Reactome: R-HSA-1462054Reactome: R-HSA-167590Reactome: R-HSA-173107Reactome: R-HSA-180534Reactome: R-HSA-202424Reactome: R-HSA-202427Reactome: R-HSA-202430Reactome: R-HSA-202433Reactome: R-HSA-389948Reactome: R-HSA-449836Reactome: R-HSA-8856825Reactome: R-HSA-8856828 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinLocalizes To Lipid RaftsRemoved From Plasma Membrane By Hiv-1 Nef Protein That Increases Clathrin-Dependent Endocytosis Of This Antigen To Target It To Lysosomal DegradationCell Surface Expression Is Also Down-Modulated By Hiv-1 Envelope Polyprotein Gp160 That Interacts WithAnd Sequesters Cd4 In The Endoplasmic Reticulum |
| Alternative Nanobody Names | Anti-T-Cell Surface Glycoprotein Cd4 nanobodyAnti-T-Cell Surface Antigen T4/Leu-3 nanobodyAnti-Cd Antigen Cd4 nanobodyAnti-CD4 nanobody |
Information sourced from Uniprot.org