Anti-CD55 antibody (Extracellular region) [M002] (STJA0005323)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenClone M002 was generated from proprietary antigen related to the extracellular region of human CD55 from the MDA-MB-231 breast cancer cell line.
General Information
| Short Description | Mouse monoclonal antibody anti-CD55 (Extracellular region) is suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation research applications. |
| Applications | WB/ICC/IP |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | M002 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Purification | Protein G Purified |
| Dilution Range | WB 1:500ICC 1:100IP 1:50 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CD55 |
| Gene ID | 1604 |
| Uniprot ID | DAF_HUMAN |
| Immunogen | Clone M002 was generated from proprietary antigen related to the extracellular region of human CD55 from the MDA-MB-231 breast cancer cell line. |
| Immunogen Region | Extracellular region |
| Specificity | Clone M002 detects 60-80 kDa* bands corresponding to the molecular mass of CD55 on SDS-PAGE immunoblots of native" A431, A549, and MDA-MB-231 cell lysates. The antibody also detects a "native" recombinant human CD55 extracellular region, but does not |
Additional Info
| Tissue Specificity | Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix. |
| Post Translational Modifications | The Ser/Thr-rich domain is heavily O-glycosylated. |
| Function | This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage. (Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5. (Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable). (Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21. |
| Protein Name | Complement Decay-Accelerating FactorCd Antigen Cd55 |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-6798695Reactome: R-HSA-6807878Reactome: R-HSA-977606 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: Cell MembraneLipid-AnchorGpi-AnchorIsoform 3: SecretedIsoform 4: SecretedIsoform 5: SecretedIsoform 6: Cell MembraneIsoform 7: Cell Membrane |
| Alternative Antibody Names | Anti-Complement Decay-Accelerating Factor antibodyAnti-Cd Antigen Cd55 antibodyAnti-CD55 antibodyAnti-CR antibodyAnti-DAF antibody |
Information sourced from Uniprot.org